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Genetics. 1998 Jul; 149(3): 1465–1475.
PMCID: PMC1460226

Drosophila hormone receptor 38 functions in metamorphosis: a role in adult cuticle formation.


DHR38 is a member of the steroid receptor superfamily in Drosophila homologous to the vertebrate NGFI-B-type orphan receptors. In addition to binding to specific response elements as a monomer, DHR38 interacts with the USP component of the ecdysone receptor complex in vitro, in yeast and in a cell line, suggesting that DHR38 might modulate ecdysone-triggered signals in the fly. We characterized the molecular structure and expression of the Dhr38 gene and initiated an in vivo analysis of its function(s) in development. The Dhr38 transcription unit spans more than 40 kb in length, includes four introns, and produces at least four mRNA isoforms differentially expressed in development; two of these are greatly enriched in the pupal stage and encode nested polypeptides. We characterized four alleles of Dhr38: a P-element enchancer trap line, l(2)02306, which shows exclusively epidermal staining in the late larval, pre-pupal and pupal stages, and three EMS-induced alleles. Dhr38 alleles cause localized fragility and rupturing of the adult cuticle, demonstrating that Dhr38 plays an important role in late stages of epidermal metamorphosis.

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Selected References

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  • Apple RT, Fristrom JW. 20-Hydroxyecdysone is required for, and negatively regulates, transcription of Drosophila pupal cuticle protein genes. Dev Biol. 1991 Aug;146(2):569–582. [PubMed]
  • Bainbridge SP, Bownes M. Staging the metamorphosis of Drosophila melanogaster. J Embryol Exp Morphol. 1981 Dec;66:57–80. [PubMed]
  • Bayer CA, von Kalm L, Fristrom JW. Relationships between protein isoforms and genetic functions demonstrate functional redundancy at the Broad-Complex during Drosophila metamorphosis. Dev Biol. 1997 Jul 15;187(2):267–282. [PubMed]
  • Castrillon DH, Wasserman SA. Diaphanous is required for cytokinesis in Drosophila and shares domains of similarity with the products of the limb deformity gene. Development. 1994 Dec;120(12):3367–3377. [PubMed]
  • Cherbas L, Cherbas P. The arthropod initiator: the capsite consensus plays an important role in transcription. Insect Biochem Mol Biol. 1993 Jan;23(1):81–90. [PubMed]
  • Roter AH, Spofford JB, Swift H. Synthesis of the major adult cuticle proteins of Drosophila melanogaster during hypoderm differentiation. Dev Biol. 1985 Feb;107(2):420–431. [PubMed]
  • Chihara CJ, Silvert DJ, Fristrom JW. The cuticle proteins of Drosophila melanogaster: stage specificity. Dev Biol. 1982 Feb;89(2):379–388. [PubMed]
  • Russell SR, Heimbeck G, Goddard CM, Carpenter AT, Ashburner M. The Drosophila Eip78C gene is not vital but has a role in regulating chromosome puffs. Genetics. 1996 Sep;144(1):159–170. [PMC free article] [PubMed]
  • Clark WC, Doctor J, Fristrom JW, Hodgetts RB. Differential responses of the dopa decarboxylase gene to 20-OH-ecdysone in Drosophila melanogaster. Dev Biol. 1986 Mar;114(1):141–150. [PubMed]
  • Ryseck RP, Macdonald-Bravo H, Mattéi MG, Ruppert S, Bravo R. Structure, mapping and expression of a growth factor inducible gene encoding a putative nuclear hormonal binding receptor. EMBO J. 1989 Nov;8(11):3327–3335. [PMC free article] [PubMed]
  • Decker CJ, Parker R. Mechanisms of mRNA degradation in eukaryotes. Trends Biochem Sci. 1994 Aug;19(8):336–340. [PubMed]
  • Erdélyi M, Máthé E, Szabad J. Genetic and developmental analysis of mutant Ketel alleles that identify the Drosophila importin-beta homologue. Acta Biol Hung. 1997;48(3):323–338. [PubMed]
  • Fechtel K, Natzle JE, Brown EE, Fristrom JW. Prepupal differentiation of Drosophila imaginal discs: identification of four genes whose transcripts accumulate in response to a pulse of 20-hydroxyecdysone. Genetics. 1988 Oct;120(2):465–474. [PMC free article] [PubMed]
  • Segraves WA, Hogness DS. The E75 ecdysone-inducible gene responsible for the 75B early puff in Drosophila encodes two new members of the steroid receptor superfamily. Genes Dev. 1990 Feb;4(2):204–219. [PubMed]
  • Fisk GJ, Thummel CS. Isolation, regulation, and DNA-binding properties of three Drosophila nuclear hormone receptor superfamily members. Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10604–10608. [PMC free article] [PubMed]
  • Shea MJ, King DL, Conboy MJ, Mariani BD, Kafatos FC. Proteins that bind to Drosophila chorion cis-regulatory elements: a new C2H2 zinc finger protein and a C2C2 steroid receptor-like component. Genes Dev. 1990 Jul;4(7):1128–1140. [PubMed]
  • Forman BM, Umesono K, Chen J, Evans RM. Unique response pathways are established by allosteric interactions among nuclear hormone receptors. Cell. 1995 May 19;81(4):541–550. [PubMed]
  • Stone BL, Thummel CS. The Drosophila 78C early late puff contains E78, an ecdysone-inducible gene that encodes a novel member of the nuclear hormone receptor superfamily. Cell. 1993 Oct 22;75(2):307–320. [PubMed]
  • Sutherland JD, Kozlova T, Tzertzinis G, Kafatos FC. Drosophila hormone receptor 38: a second partner for Drosophila USP suggests an unexpected role for nuclear receptors of the nerve growth factor-induced protein B type. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7966–7970. [PMC free article] [PubMed]
  • Hiruma K, Hardie J, Riddiford LM. Hormonal regulation of epidermal metamorphosis in vitro: control of expression of a larval-specific cuticle gene. Dev Biol. 1991 Apr;144(2):369–378. [PubMed]
  • Talbot WS, Swyryd EA, Hogness DS. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms. Cell. 1993 Jul 2;73(7):1323–1337. [PubMed]
  • Thomas HE, Stunnenberg HG, Stewart AF. Heterodimerization of the Drosophila ecdysone receptor with retinoid X receptor and ultraspiracle. Nature. 1993 Apr 1;362(6419):471–475. [PubMed]
  • Koelle MR, Segraves WA, Hogness DS. DHR3: a Drosophila steroid receptor homolog. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6167–6171. [PMC free article] [PubMed]
  • Thummel CS. From embryogenesis to metamorphosis: the regulation and function of Drosophila nuclear receptor superfamily members. Cell. 1995 Dec 15;83(6):871–877. [PubMed]
  • Kraminsky GP, Clark WC, Estelle MA, Gietz RD, Sage BA, O'Connor JD, Hodgetts RB. Induction of translatable mRNA for dopa decarboxylase in Drosophila: an early response to ecdysterone. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4175–4179. [PMC free article] [PubMed]
  • Thummel CS. Dueling orphans--interacting nuclear receptors coordinate Drosophila metamorphosis. Bioessays. 1997 Aug;19(8):669–672. [PubMed]
  • van de Wetering M, Cavallo R, Dooijes D, van Beest M, van Es J, Loureiro J, Ypma A, Hursh D, Jones T, Bejsovec A, et al. Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF. Cell. 1997 Mar 21;88(6):789–799. [PubMed]
  • Moore GD, Sinclair DA, Grigliatti TA. Histone Gene Multiplicity and Position Effect Variegation in DROSOPHILA MELANOGASTER. Genetics. 1983 Oct;105(2):327–344. [PMC free article] [PubMed]
  • Watson MA, Milbrandt J. The NGFI-B gene, a transcriptionally inducible member of the steroid receptor gene superfamily: genomic structure and expression in rat brain after seizure induction. Mol Cell Biol. 1989 Oct;9(10):4213–4219. [PMC free article] [PubMed]
  • O'Connell PO, Rosbash M. Sequence, structure, and codon preference of the Drosophila ribosomal protein 49 gene. Nucleic Acids Res. 1984 Jul 11;12(13):5495–5513. [PMC free article] [PubMed]
  • Wright TR. The Wilhelmine E. Key 1992 Invitational lecture. Phenotypic analysis of the Dopa decarboxylase gene cluster mutants in Drosophila melanogaster. J Hered. 1996 May-Jun;87(3):175–190. [PubMed]
  • Ohkura N, Ito M, Tsukada T, Sasaki K, Yamaguchi K, Miki K. Structure, mapping and expression of a human NOR-1 gene, the third member of the Nur77/NGFI-B family. Biochim Biophys Acta. 1996 Sep 11;1308(3):205–214. [PubMed]
  • Yao TP, Forman BM, Jiang Z, Cherbas L, Chen JD, McKeown M, Cherbas P, Evans RM. Functional ecdysone receptor is the product of EcR and Ultraspiracle genes. Nature. 1993 Dec 2;366(6454):476–479. [PubMed]
  • Perlmann T, Jansson L. A novel pathway for vitamin A signaling mediated by RXR heterodimerization with NGFI-B and NURR1. Genes Dev. 1995 Apr 1;9(7):769–782. [PubMed]
  • Zetterström RH, Solomin L, Mitsiadis T, Olson L, Perlmann T. Retinoid X receptor heterodimerization and developmental expression distinguish the orphan nuclear receptors NGFI-B, Nurr1, and Nor1. Mol Endocrinol. 1996 Dec;10(12):1656–1666. [PubMed]
  • Perlmann T, Jansson L. A novel pathway for vitamin A signaling mediated by RXR heterodimerization with NGFI-B and NURR1. Genes Dev. 1995 Apr 1;9(7):769–782. [PubMed]
  • Qiu J, Hardin PE. Temporal and spatial expression of an adult cuticle protein gene from Drosophila suggests that its protein product may impart some specialized cuticle function. Dev Biol. 1995 Feb;167(2):416–425. [PubMed]
  • Zetterström RH, Solomin L, Jansson L, Hoffer BJ, Olson L, Perlmann T. Dopamine neuron agenesis in Nurr1-deficient mice. Science. 1997 Apr 11;276(5310):248–250. [PubMed]

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