• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Feb 1998; 148(2): 581–598.
PMCID: PMC1459833

Yeast meiotic mutants proficient for the induction of ectopic recombination.

Abstract

A screen was designed to identify Saccharomyces cerevisiae mutants that were defective in meiosis yet proficient for meiotic ectopic recombination in the return-to-growth protocol. Seven mutants alleles were isolated; two are important for chromosome synapsis (RED1, MEK1) and five function independently of recombination (SPO14, GSG1, SPOT8/MUM2, 3, 4). Similar to the spoT8-1 mutant, mum2 deletion strains do not undergo premeiotic DNA synthesis, arrest prior to the first meiotic division and fail to sporulate. Surprisingly, although DNA replication does not occur, mum2 mutants are induced for high levels of ectopic recombination. gsg1 diploids are reduced in their ability to complete premeiotic DNA synthesis and the meiotic divisions, and a small percentage of cells produce spores. mum3 mutants sporulate poorly and the spores produced are inviable. Finally, mum4-1 mutants produce inviable spores. The meiotic/sporulation defects of gsg1, mum2, and mum3 are not relieved by spo11 or spo13 mutations, indicating that the mutant defects are not dependent on the initiation of recombination or completion of both meiotic divisions. In contrast, the spore inviability of the mum4-1 mutant is rescued by the spo13 mutation. The mum4-1 spo13 mutant undergoes a single, predominantly equational division, suggesting that MUM4 functions at or prior to the first meiotic division. Although recombination is variably affected in the gsg1 and mum mutants, we hypothesize that these mutants define genes important for aspects of meiosis not directly related to recombination.

Full Text

The Full Text of this article is available as a PDF (268K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ajimura M, Leem SH, Ogawa H. Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae. Genetics. 1993 Jan;133(1):51–66. [PMC free article] [PubMed]
  • Alani E, Cao L, Kleckner N. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics. 1987 Aug;116(4):541–545. [PMC free article] [PubMed]
  • Bhargava J, Engebrecht J, Roeder GS. The rec102 mutant of yeast is defective in meiotic recombination and chromosome synapsis. Genetics. 1992 Jan;130(1):59–69. [PMC free article] [PubMed]
  • Bishop DK, Park D, Xu L, Kleckner N. DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell. 1992 May 1;69(3):439–456. [PubMed]
  • Borts RH, Lichten M, Haber JE. Analysis of meiosis-defective mutations in yeast by physical monitoring of recombination. Genetics. 1986 Jul;113(3):551–567. [PMC free article] [PubMed]
  • Callan HG. Replication of DNA in the chromosomes of eukaryotes. Proc R Soc Lond B Biol Sci. 1972 Apr 18;181(1062):19–41. [PubMed]
  • Chua PR, Roeder GS. Tam1, a telomere-associated meiotic protein, functions in chromosome synapsis and crossover interference. Genes Dev. 1997 Jul 15;11(14):1786–1800. [PubMed]
  • Collins I, Newlon CS. Chromosomal DNA replication initiates at the same origins in meiosis and mitosis. Mol Cell Biol. 1994 May;14(5):3524–3534. [PMC free article] [PubMed]
  • Conrad MN, Dominguez AM, Dresser ME. Ndj1p, a meiotic telomere protein required for normal chromosome synapsis and segregation in yeast. Science. 1997 May 23;276(5316):1252–1255. [PubMed]
  • Dawes IW, Hardie ID. Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Mol Gen Genet. 1974;131(4):281–289. [PubMed]
  • Elledge SJ, Davis RW. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae. Gene. 1988 Oct 30;70(2):303–312. [PubMed]
  • Engebrecht J, Hirsch J, Roeder GS. Meiotic gene conversion and crossing over: their relationship to each other and to chromosome synapsis and segregation. Cell. 1990 Sep 7;62(5):927–937. [PubMed]
  • Engebrecht J, Roeder GS. Yeast mer1 mutants display reduced levels of meiotic recombination. Genetics. 1989 Feb;121(2):237–247. [PMC free article] [PubMed]
  • Esposito MS, Esposito RE. Genes controlling meiosis and spore formation in yeast. Genetics. 1974 Sep;78(1):215–225. [PMC free article] [PubMed]
  • Esposito RE, Esposito MS. Genetic recombination and commitment to meiosis in Saccharomyces. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3172–3176. [PMC free article] [PubMed]
  • Feldmann H, Aigle M, Aljinovic G, André B, Baclet MC, Barthe C, Baur A, Bécam AM, Biteau N, Boles E, et al. Complete DNA sequence of yeast chromosome II. EMBO J. 1994 Dec 15;13(24):5795–5809. [PMC free article] [PubMed]
  • Fleig UN, Pridmore RD, Philippsen P. Construction of LYS2 cartridges for use in genetic manipulations of Saccharomyces cerevisiae. Gene. 1986;46(2-3):237–245. [PubMed]
  • Fogel S, Hurst DD. Meiotic gene conversion in yeast tetrads and the theory of recombination. Genetics. 1967 Oct;57(2):455–481. [PMC free article] [PubMed]
  • Goldman AS, Lichten M. The efficiency of meiotic recombination between dispersed sequences in Saccharomyces cerevisiae depends upon their chromosomal location. Genetics. 1996 Sep;144(1):43–55. [PMC free article] [PubMed]
  • Goyon C, Lichten M. Timing of molecular events in meiosis in Saccharomyces cerevisiae: stable heteroduplex DNA is formed late in meiotic prophase. Mol Cell Biol. 1993 Jan;13(1):373–382. [PMC free article] [PubMed]
  • Haber JE, Leung WY, Borts RH, Lichten M. The frequency of meiotic recombination in yeast is independent of the number and position of homologous donor sequences: implications for chromosome pairing. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1120–1124. [PMC free article] [PubMed]
  • Hill JE, Myers AM, Koerner TJ, Tzagoloff A. Yeast/E. coli shuttle vectors with multiple unique restriction sites. Yeast. 1986 Sep;2(3):163–167. [PubMed]
  • Hollingsworth NM, Byers B. HOP1: a yeast meiotic pairing gene. Genetics. 1989 Mar;121(3):445–462. [PMC free article] [PubMed]
  • Hollingsworth NM, Goetsch L, Byers B. The HOP1 gene encodes a meiosis-specific component of yeast chromosomes. Cell. 1990 Apr 6;61(1):73–84. [PubMed]
  • Hollingsworth NM, Ponte L. Genetic interactions between HOP1, RED1 and MEK1 suggest that MEK1 regulates assembly of axial element components during meiosis in the yeast Saccharomyces cerevisiae. Genetics. 1997 Sep;147(1):33–42. [PMC free article] [PubMed]
  • Hollingsworth NM, Ponte L, Halsey C. MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination between homologs in Saccharomyces cerevisiae but not mismatch repair. Genes Dev. 1995 Jul 15;9(14):1728–1739. [PubMed]
  • Honigberg SM, Conicella C, Espositio RE. Commitment to meiosis in Saccharomyces cerevisiae: involvement of the SPO14 gene. Genetics. 1992 Apr;130(4):703–716. [PMC free article] [PubMed]
  • Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. [PMC free article] [PubMed]
  • Jinks-Robertson S, Petes TD. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes. Genetics. 1986 Nov;114(3):731–752. [PMC free article] [PubMed]
  • Johnston LH, Williamson DH, Johnson AL, Fennell DJ. On the mechanism of premeiotic DNA synthesis in the yeast Saccharomyces cerevisiae. Exp Cell Res. 1982 Sep;141(1):53–62. [PubMed]
  • Kaytor MD, Livingston DM. GSG1, a yeast gene required for sporulation. Yeast. 1995 Sep 30;11(12):1147–1155. [PubMed]
  • Keeney S, Giroux CN, Kleckner N. Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell. 1997 Feb 7;88(3):375–384. [PubMed]
  • Klapholz S, Esposito RE. Recombination and chromosome segregation during the single division meiosis in SPO12-1 and SPO13-1 diploids. Genetics. 1980 Nov;96(3):589–611. [PMC free article] [PubMed]
  • Klapholz S, Waddell CS, Esposito RE. The role of the SPO11 gene in meiotic recombination in yeast. Genetics. 1985 Jun;110(2):187–216. [PMC free article] [PubMed]
  • Kleckner N. Meiosis: how could it work? Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8167–8174. [PMC free article] [PubMed]
  • Kobayashi T, Hotta Y, Tabata S. Isolation and characterization of a yeast gene that is homologous with a meiosis-specific cDNA from a plant. Mol Gen Genet. 1993 Feb;237(1-2):225–232. [PubMed]
  • Kofman-Alfaro S, Chandley AC. Meiosis in the male mouse. An autoradiographic investigation. Chromosoma. 1970;31(4):404–420. [PubMed]
  • Leem SH, Ogawa H. The MRE4 gene encodes a novel protein kinase homologue required for meiotic recombination in Saccharomyces cerevisiae. Nucleic Acids Res. 1992 Feb 11;20(3):449–457. [PMC free article] [PubMed]
  • Lichten M, Borts RH, Haber JE. Meiotic gene conversion and crossing over between dispersed homologous sequences occurs frequently in Saccharomyces cerevisiae. Genetics. 1987 Feb;115(2):233–246. [PMC free article] [PubMed]
  • Loidl J, Klein F, Scherthan H. Homologous pairing is reduced but not abolished in asynaptic mutants of yeast. J Cell Biol. 1994 Jun;125(6):1191–1200. [PMC free article] [PubMed]
  • Lydall D, Nikolsky Y, Bishop DK, Weinert T. A meiotic recombination checkpoint controlled by mitotic checkpoint genes. Nature. 1996 Oct 31;383(6603):840–843. [PubMed]
  • Malone RE, Bullard S, Hermiston M, Rieger R, Cool M, Galbraith A. Isolation of mutants defective in early steps of meiotic recombination in the yeast Saccharomyces cerevisiae. Genetics. 1991 May;128(1):79–88. [PMC free article] [PubMed]
  • McCarroll RM, Esposito RE. SPO13 negatively regulates the progression of mitotic and meiotic nuclear division in Saccharomyces cerevisiae. Genetics. 1994 Sep;138(1):47–60. [PMC free article] [PubMed]
  • McKee AH, Kleckner N. A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics. 1997 Jul;146(3):797–816. [PMC free article] [PubMed]
  • McKee AH, Kleckner N. Mutations in Saccharomyces cerevisiae that block meiotic prophase chromosome metabolism and confer cell cycle arrest at pachytene identify two new meiosis-specific genes SAE1 and SAE3. Genetics. 1997 Jul;146(3):817–834. [PMC free article] [PubMed]
  • Padmore R, Cao L, Kleckner N. Temporal comparison of recombination and synaptonemal complex formation during meiosis in S. cerevisiae. Cell. 1991 Sep 20;66(6):1239–1256. [PubMed]
  • Prinz S, Amon A, Klein F. Isolation of COM1, a new gene required to complete meiotic double-strand break-induced recombination in Saccharomyces cerevisiae. Genetics. 1997 Jul;146(3):781–795. [PMC free article] [PubMed]
  • Rockmill B, Roeder GS. RED1: a yeast gene required for the segregation of chromosomes during the reductional division of meiosis. Proc Natl Acad Sci U S A. 1988 Aug;85(16):6057–6061. [PMC free article] [PubMed]
  • Rockmill B, Roeder GS. Meiosis in asynaptic yeast. Genetics. 1990 Nov;126(3):563–574. [PMC free article] [PubMed]
  • Rockmill B, Roeder GS. A meiosis-specific protein kinase homolog required for chromosome synapsis and recombination. Genes Dev. 1991 Dec;5(12B):2392–2404. [PubMed]
  • Roeder GS. Sex and the single cell: meiosis in yeast. Proc Natl Acad Sci U S A. 1995 Nov 7;92(23):10450–10456. [PMC free article] [PubMed]
  • Rose K, Rudge SA, Frohman MA, Morris AJ, Engebrecht J. Phospholipase D signaling is essential for meiosis. Proc Natl Acad Sci U S A. 1995 Dec 19;92(26):12151–12155. [PMC free article] [PubMed]
  • Rose MD, Novick P, Thomas JH, Botstein D, Fink GR. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene. 1987;60(2-3):237–243. [PubMed]
  • Ross-Macdonald P, Roeder GS. Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction. Cell. 1994 Dec 16;79(6):1069–1080. [PubMed]
  • Roth R. Chromosome replication during meiosis: identification of gene functions required for premeiotic DNA synthesis. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3087–3091. [PMC free article] [PubMed]
  • Rothstein R. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 1991;194:281–301. [PubMed]
  • Sazer S, Sherwood SW. Mitochondrial growth and DNA synthesis occur in the absence of nuclear DNA replication in fission yeast. J Cell Sci. 1990 Nov;97(Pt 3):509–516. [PubMed]
  • Seifert HS, Chen EY, So M, Heffron F. Shuttle mutagenesis: a method of transposon mutagenesis for Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1986 Feb;83(3):735–739. [PMC free article] [PubMed]
  • Smith AV, Roeder GS. The yeast Red1 protein localizes to the cores of meiotic chromosomes. J Cell Biol. 1997 Mar 10;136(5):957–967. [PMC free article] [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Steele DF, Morris ME, Jinks-Robertson S. Allelic and ectopic interactions in recombination-defective yeast strains. Genetics. 1991 Jan;127(1):53–60. [PMC free article] [PubMed]
  • Sym M, Engebrecht JA, Roeder GS. ZIP1 is a synaptonemal complex protein required for meiotic chromosome synapsis. Cell. 1993 Feb 12;72(3):365–378. [PubMed]
  • Tsuboi M. The isolation and genetic analysis of sporulation-deficient mutants in Saccharomyces cerevisiae. Mol Gen Genet. 1983;191(1):17–21. [PubMed]
  • Vershon AK, Hollingsworth NM, Johnson AD. Meiotic induction of the yeast HOP1 gene is controlled by positive and negative regulatory sites. Mol Cell Biol. 1992 Sep;12(9):3706–3714. [PMC free article] [PubMed]
  • von Wettstein D, Rasmussen SW, Holm PB. The synaptonemal complex in genetic segregation. Annu Rev Genet. 1984;18:331–413. [PubMed]
  • Weiner BM, Kleckner N. Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast. Cell. 1994 Jul 1;77(7):977–991. [PubMed]
  • Williamson DH, Johnston LH, Fennell DJ, Simchen G. The timing of the S phase and other nuclear events in yeast meiosis. Exp Cell Res. 1983 Apr 15;145(1):209–217. [PubMed]
  • Xu L, Ajimura M, Padmore R, Klein C, Kleckner N. NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae. Mol Cell Biol. 1995 Dec;15(12):6572–6581. [PMC free article] [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...