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Nucleic Acids Res. Jun 1, 1996; 24(11): 2119–2124.
PMCID: PMC145910

A multi-well version of in situ hybridization on whole mount embryos of Caenorhabditis elegans.

Abstract

We report an efficient procedure for in situ hybridization with a multi-well format on Caenorhabditis elegans embryos for large scale screening of gene expression patterns in this organism. Each hybridization well contains embryos at various stages throughout embryogenesis. The validity of the method was confirmed through results with control genes whose expression patterns have been reported; glp-1 in very early embryos, myo-2 in pharyngeal muscle and unc-54 in body wall muscle. Several collagen genes and a pepsinogen gene were also examined to establish a set of lineage-specific markers. As a pilot project, we examined approximately 100 unique cDNA species classified by our cDNA project, finding that approximately 10% of the cDNA groups were expressed in specific cells and at specific stages.

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Selected References

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  • Sulston JE, Schierenberg E, White JG, Thomson JN. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev Biol. 1983 Nov;100(1):64–119. [PubMed]
  • Evans TC, Crittenden SL, Kodoyianni V, Kimble J. Translational control of maternal glp-1 mRNA establishes an asymmetry in the C. elegans embryo. Cell. 1994 Apr 22;77(2):183–194. [PubMed]
  • Seydoux G, Fire A. Soma-germline asymmetry in the distributions of embryonic RNAs in Caenorhabditis elegans. Development. 1994 Oct;120(10):2823–2834. [PubMed]
  • Birchall PS, Fishpool RM, Albertson DG. Expression patterns of predicted genes from the C. elegans genome sequence visualized by FISH in whole organisms. Nat Genet. 1995 Nov;11(3):314–320. [PubMed]
  • Hope IA. 'Promoter trapping' in Caenorhabditis elegans. Development. 1991 Oct;113(2):399–408. [PubMed]
  • Lynch AS, Briggs D, Hope IA. Developmental expression pattern screen for genes predicted in the C. elegans genome sequencing project. Nat Genet. 1995 Nov;11(3):309–313. [PubMed]
  • Sulston J, Du Z, Thomas K, Wilson R, Hillier L, Staden R, Halloran N, Green P, Thierry-Mieg J, Qiu L, et al. The C. elegans genome sequencing project: a beginning. Nature. 1992 Mar 5;356(6364):37–41. [PubMed]
  • Wilson R, Ainscough R, Anderson K, Baynes C, Berks M, Bonfield J, Burton J, Connell M, Copsey T, Cooper J, et al. 2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans. Nature. 1994 Mar 3;368(6466):32–38. [PubMed]
  • Cox GN, Kramer JM, Hirsh D. Number and organization of collagen genes in Caenorhabditis elegans. Mol Cell Biol. 1984 Nov;4(11):2389–2395. [PMC free article] [PubMed]
  • Waterston R, Martin C, Craxton M, Huynh C, Coulson A, Hillier L, Durbin R, Green P, Shownkeen R, Halloran N, et al. A survey of expressed genes in Caenorhabditis elegans. Nat Genet. 1992 May;1(2):114–123. [PubMed]
  • Larin Z, Fricker MD, Maher E, Ishikawa-Brush Y, Southern EM. Fluorescence in situ hybridisation of multiple probes on a single microscope slide. Nucleic Acids Res. 1994 Sep 11;22(18):3689–3692. [PMC free article] [PubMed]
  • Ardizzi JP, Epstein HF. Immunochemical localization of myosin heavy chain isoforms and paramyosin in developmentally and structurally diverse muscle cell types of the nematode Caenorhabditis elegans. J Cell Biol. 1987 Dec;105(6 Pt 1):2763–2770. [PMC free article] [PubMed]
  • Guo XD, Kramer JM. The two Caenorhabditis elegans basement membrane (type IV) collagen genes are located on separate chromosomes. J Biol Chem. 1989 Oct 15;264(29):17574–17582. [PubMed]
  • Cox GN. Molecular and biochemical aspects of nematode collagens. J Parasitol. 1992 Feb;78(1):1–15. [PubMed]
  • Zwaal RR, Broeks A, van Meurs J, Groenen JT, Plasterk RH. Target-selected gene inactivation in Caenorhabditis elegans by using a frozen transposon insertion mutant bank. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7431–7435. [PMC free article] [PubMed]

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