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Immunology. Oct 1985; 56(2): 351–358.
PMCID: PMC1453689

Macrophage recognition of cells undergoing programmed cell death (apoptosis).

Abstract

As a model for the recognition of effete cells by their viable neighbours. BALB/c mouse thymocytes were coincubated with isologous peritoneal macrophages. The macrophages bound preferentially to thymocytes undergoing apoptosis, a mode of death induced in these cells by treatment with the glucocorticoid hormone methyl-prednisolone. Binding occurred in the absence of serum and was inhibited by N,N'-diacetyl chitobiose, N-acetyl glucosamine and, to a lesser extent, by N-acetyl galactosamine and D-galactose. L-fucose, D-mannose and N-acetyl neuraminic acid had no effect. The results suggest the presence of lectin-like molecules on the surface of the macrophage that recognize changes in the cell-surface carbohydrate of the apoptotic cell. The pattern of inhibition of binding by monosaccharides differs from that of previously described endogenous mammalian lectins.

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Selected References

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  • Brown WR, Barclay AN, Sunderland CA, Williams AF. Identification of a glycophorin-like molecule at the cell surface of rat thymocytes. Nature. 1981 Feb 5;289(5797):456–460. [PubMed]
  • Brown WR, Williams AF. Lymphocyte cell surface glycoproteins which bind to soybean and peanut lectins. Immunology. 1982 Aug;46(4):713–726. [PMC free article] [PubMed]
  • Colten HR, Borsos T, Rapp HJ. In vitro synthesis of the first component of complement by guinea pig small intestine. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1158–1163. [PMC free article] [PubMed]
  • Ferguson DJ, Anderson TJ. Morphological evaluation of cell turnover in relation to the menstrual cycle in the "resting" human breast. Br J Cancer. 1981 Aug;44(2):177–181. [PMC free article] [PubMed]
  • Gahmberg CG, Häyry P, Andersson LC. Characterization of surface glycoproteins of mouse lymphoid cells. J Cell Biol. 1976 Mar;68(3):642–653. [PMC free article] [PubMed]
  • Galili U, Leizerowitz R, Moreb J, Gamliel H, Gurfel D, Polliack A. Metabolic and ultrastructural aspects of the in vitro lysis of chronic lymphocytic leukemia cells by glucocorticoids. Cancer Res. 1982 Apr;42(4):1433–1440. [PubMed]
  • Geiger H, Day N, Good RA. Ontogenetic development and synthesis of hemolytic C8 by piglet tissues. J Immunol. 1972 Apr;108(4):1092–1097. [PubMed]
  • Hedgecock EM, Sulston JE, Thomson JN. Mutations affecting programmed cell deaths in the nematode Caenorhabditis elegans. Science. 1983 Jun 17;220(4603):1277–1279. [PubMed]
  • Hudgin RL, Pricer WE, Jr, Ashwell G, Stockert RJ, Morell AG. The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins. J Biol Chem. 1974 Sep 10;249(17):5536–5543. [PubMed]
  • Hurle JM, Lafarga M, Hinchliffe JR. The surface coat of embryonic limb mesenchymal cells during morphogenetic cell death. Exp Cell Res. 1981 Jun;133(2):465–470. [PubMed]
  • Joel DD, Chanana AD, Cottier H, Cronkite EP, Laissue JA. Fate of thymocytes: studies with 125I-iododeoxyuridine and 3H-thymidine in mice. Cell Tissue Kinet. 1977 Jan;10(1):57–69. [PubMed]
  • Kawasaki T, Etoh R, Yamashina I. Isolation and characterization of a mannan-binding protein from rabbit liver. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1018–1024. [PubMed]
  • Kay MM. Mechanism of removal of senescent cells by human macrophages in situ. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3521–3525. [PMC free article] [PubMed]
  • Kay MM. Isolation of the phagocytosis-inducing IgG-binding antigen on senescent somatic cells. Nature. 1981 Feb 5;289(5797):491–494. [PubMed]
  • Morris RG, Hargreaves AD, Duvall E, Wyllie AH. Hormone-induced cell death. 2. Surface changes in thymocytes undergoing apoptosis. Am J Pathol. 1984 Jun;115(3):426–436. [PMC free article] [PubMed]
  • Nagamura Y, Kolb H. Presence of a lectin-like receptor for D-galactose on rat peritoneal macrophages. FEBS Lett. 1980 Jun 16;115(1):59–62. [PubMed]
  • Newman SL, Henson JE, Henson PM. Phagocytosis of senescent neutrophils by human monocyte-derived macrophages and rabbit inflammatory macrophages. J Exp Med. 1982 Aug 1;156(2):430–442. [PMC free article] [PubMed]
  • Page RC, Davies P, Allison AC. The macrophage as a secretory cell. Int Rev Cytol. 1978;52:119–157. [PubMed]
  • Prieels JP, Pizzo SV, Glasgow LR, Paulson JC, Hill RL. Hepatic receptor that specifically binds oligosaccharides containing fucosyl alpha1 leads to 3 N-acetylglucosamine linkages. Proc Natl Acad Sci U S A. 1978 May;75(5):2215–2219. [PMC free article] [PubMed]
  • Rommel FA, Goldlust MB, Bancroft FC, Mayer MM, Tashjian AH., Jr Synthesis of the ninth component of complement by a clonal strain of rat hepatoma cells. J Immunol. 1970 Aug;105(2):396–403. [PubMed]
  • Ruddy S, Colten HR. Rheumatoid arthritis. Biosynthesis of complement proteins by synovial tissues. N Engl J Med. 1974 Jun 6;290(23):1284–1288. [PubMed]
  • Sandow BA, West NB, Norman RL, Brenner RM. Hormonal control of apoptosis in hamster uterine luminal epithelium. Am J Anat. 1979 Sep;156(1):15–35. [PubMed]
  • Schlepper-Schäfer J, Kolb-Bachofen V, Kolb H. Analysis of lectin-dependent recognition of desialylated erythrocytes by Kupffer cells. Biochem J. 1980 Mar 15;186(3):827–831. [PMC free article] [PubMed]
  • Stockert RJ, Morell AG, Scheinberg IH. The existence of a second route for the transfer of certain glycoproteins from the circulation into the liver. Biochem Biophys Res Commun. 1976 Feb 9;68(3):988–993. [PubMed]
  • Weir DM, Ogmundsdóttir HM. Non-specific recognition mechanisms by mononuclear phagocytes. Clin Exp Immunol. 1977 Nov;30(2):323–329. [PMC free article] [PubMed]
  • Wright NA, Voncina D, Morley AR. An attempt to demonstrate cell migration from the zona glomerulosa in the prepubertal male rat adrenal cortex. J Endocrinol. 1973 Dec;59(3):451–459. [PubMed]
  • Wyatt HV, Colten HR, Borsos T. Production of the second (C2) and fourth (C4) components of guinea pig complement by single peritoneal cells: evidence that one cell may produce both components. J Immunol. 1972 Jun;108(6):1609–1614. [PubMed]
  • Wyllie AH. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 1980 Apr 10;284(5756):555–556. [PubMed]
  • Wyllie AH, Kerr JF, Currie AR. Cell death in the normal neonatal rat adrenal cortex. J Pathol. 1973 Dec;111(4):255–261. [PubMed]
  • Wyllie AH, Kerr JF, Currie AR. Cell death: the significance of apoptosis. Int Rev Cytol. 1980;68:251–306. [PubMed]
  • Wyllie AH, Kerr JF, Macaskill IA, Currie AR. Adrenocortical cell deletion: the role of ACTH. J Pathol. 1973 Oct;111(2):85–94. [PubMed]
  • Wyllie AH, Morris RG. Hormone-induced cell death. Purification ad properties of thymocytes undergoing apoptosis after glucocorticoid treatment. Am J Pathol. 1982 Oct;109(1):78–87. [PMC free article] [PubMed]
  • Wyllie AH, Morris RG, Smith AL, Dunlop D. Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol. 1984 Jan;142(1):67–77. [PubMed]
  • Yamada T, Ohyama H. Changes in surface morphology of rat thymocytes accompanying interphase death. J Radiat Res. 1980 Jun;21(2):190–196. [PubMed]

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