• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Dec 2001; 159(4): 1491–1499.
PMCID: PMC1450841

Phosphate transport and sensing in Saccharomyces cerevisiae.

Abstract

Cellular metabolism depends on the appropriate concentration of intracellular inorganic phosphate; however, little is known about how phosphate concentrations are sensed. The similarity of Pho84p, a high-affinity phosphate transporter in Saccharomyces cerevisiae, to the glucose sensors Snf3p and Rgt2p has led to the hypothesis that Pho84p is an inorganic phosphate sensor. Furthermore, pho84Delta strains have defects in phosphate signaling; they constitutively express PHO5, a phosphate starvation-inducible gene. We began these studies to determine the role of phosphate transporters in signaling phosphate starvation. Previous experiments demonstrated a defect in phosphate uptake in phosphate-starved pho84Delta cells; however, the pho84Delta strain expresses PHO5 constitutively when grown in phosphate-replete media. We determined that pho84Delta cells have a significant defect in phosphate uptake even when grown in high phosphate media. Overexpression of unrelated phosphate transporters or a glycerophosphoinositol transporter in the pho84Delta strain suppresses the PHO5 constitutive phenotype. These data suggest that PHO84 is not required for sensing phosphate. We further characterized putative phosphate transporters, identifying two new phosphate transporters, PHO90 and PHO91. A synthetic lethal phenotype was observed when five phosphate transporters were inactivated, and the contribution of each transporter to uptake in high phosphate conditions was determined. Finally, a PHO84-dependent compensation response was identified; the abundance of Pho84p at the plasma membrane increases in cells that are defective in other phosphate transporters.

Full Text

The Full Text of this article is available as a PDF (188K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Haswell ES, O'Shea EK. An in vitro system recapitulates chromatin remodeling at the PHO5 promoter. Mol Cell Biol. 1999 Apr;19(4):2817–2827. [PMC free article] [PubMed]
  • Huang S, Jeffery DA, Anthony MD, O'Shea EK. Functional analysis of the cyclin-dependent kinase inhibitor Pho81 identifies a novel inhibitory domain. Mol Cell Biol. 2001 Oct;21(19):6695–6705. [PMC free article] [PubMed]
  • Kaffman A, Herskowitz I, Tjian R, O'Shea EK. Phosphorylation of the transcription factor PHO4 by a cyclin-CDK complex, PHO80-PHO85. Science. 1994 Feb 25;263(5150):1153–1156. [PubMed]
  • Kitada K, Yamaguchi E, Arisawa M. Cloning of the Candida glabrata TRP1 and HIS3 genes, and construction of their disruptant strains by sequential integrative transformation. Gene. 1995 Nov 20;165(2):203–206. [PubMed]
  • Kruckeberg AL, Walsh MC, Van Dam K. How do yeast cells sense glucose? Bioessays. 1998 Dec;20(12):972–976. [PubMed]
  • Lau WT, Howson RW, Malkus P, Schekman R, O'Shea EK. Pho86p, an endoplasmic reticulum (ER) resident protein in Saccharomyces cerevisiae, is required for ER exit of the high-affinity phosphate transporter Pho84p. Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1107–1112. [PMC free article] [PubMed]
  • Lau WW, Schneider KR, O'Shea EK. A genetic study of signaling processes for repression of PHO5 transcription in Saccharomyces cerevisiae. Genetics. 1998 Dec;150(4):1349–1359. [PMC free article] [PubMed]
  • Lemire JM, Willcocks T, Halvorson HO, Bostian KA. Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae. Mol Cell Biol. 1985 Aug;5(8):2131–2141. [PMC free article] [PubMed]
  • Lenburg ME, O'Shea EK. Signaling phosphate starvation. Trends Biochem Sci. 1996 Oct;21(10):383–387. [PubMed]
  • Liang H, Gaber RF. A novel signal transduction pathway in Saccharomyces cerevisiae defined by Snf3-regulated expression of HXT6. Mol Biol Cell. 1996 Dec;7(12):1953–1966. [PMC free article] [PubMed]
  • Liu H, Krizek J, Bretscher A. Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast. Genetics. 1992 Nov;132(3):665–673. [PMC free article] [PubMed]
  • Longtine MS, McKenzie A, 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast. 1998 Jul;14(10):953–961. [PubMed]
  • Berhe A, Fristedt U, Persson BL. Expression and purification of the high-affinity phosphate transporter of Saccharomyces cerevisiae. Eur J Biochem. 1995 Jan 15;227(1-2):566–572. [PubMed]
  • Martinez P, Persson BL. Identification, cloning and characterization of a derepressible Na+-coupled phosphate transporter in Saccharomyces cerevisiae. Mol Gen Genet. 1998 Jun;258(6):628–638. [PubMed]
  • Bun-Ya M, Nishimura M, Harashima S, Oshima Y. The PHO84 gene of Saccharomyces cerevisiae encodes an inorganic phosphate transporter. Mol Cell Biol. 1991 Jun;11(6):3229–3238. [PMC free article] [PubMed]
  • Muchhal US, Pardo JM, Raghothama KG. Phosphate transporters from the higher plant Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10519–10523. [PMC free article] [PubMed]
  • Bun-ya M, Shikata K, Nakade S, Yompakdee C, Harashima S, Oshima Y. Two new genes, PHO86 and PHO87, involved in inorganic phosphate uptake in Saccharomyces cerevisiae. Curr Genet. 1996 Mar;29(4):344–351. [PubMed]
  • Ogawa N, Noguchi K, Sawai H, Yamashita Y, Yompakdee C, Oshima Y. Functional domains of Pho81p, an inhibitor of Pho85p protein kinase, in the transduction pathway of Pi signals in Saccharomyces cerevisiae. Mol Cell Biol. 1995 Feb;15(2):997–1004. [PMC free article] [PubMed]
  • Carroll AS, Bishop AC, DeRisi JL, Shokat KM, O'Shea EK. Chemical inhibition of the Pho85 cyclin-dependent kinase reveals a role in the environmental stress response. Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12578–12583. [PMC free article] [PubMed]
  • Christianson TW, Sikorski RS, Dante M, Shero JH, Hieter P. Multifunctional yeast high-copy-number shuttle vectors. Gene. 1992 Jan 2;110(1):119–122. [PubMed]
  • Coons DM, Vagnoli P, Bisson LF. The C-terminal domain of Snf3p is sufficient to complement the growth defect of snf3 null mutations in Saccharomyces cerevisiae: SNF3 functions in glucose recognition. Yeast. 1997 Jan;13(1):9–20. [PubMed]
  • Ozcan S, Dover J, Rosenwald AG, Wölfl S, Johnston M. Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12428–12432. [PMC free article] [PubMed]
  • Costanzo MC, Crawford ME, Hirschman JE, Kranz JE, Olsen P, Robertson LS, Skrzypek MS, Braun BR, Hopkins KL, Kondu P, et al. YPD, PombePD and WormPD: model organism volumes of the BioKnowledge library, an integrated resource for protein information. Nucleic Acids Res. 2001 Jan 1;29(1):75–79. [PMC free article] [PubMed]
  • Ozcan S, Dover J, Johnston M. Glucose sensing and signaling by two glucose receptors in the yeast Saccharomyces cerevisiae. EMBO J. 1998 May 1;17(9):2566–2573. [PMC free article] [PubMed]
  • Cox GB, Webb D, Godovac-Zimmermann J, Rosenberg H. Arg-220 of the PstA protein is required for phosphate transport through the phosphate-specific transport system in Escherichia coli but not for alkaline phosphatase repression. J Bacteriol. 1988 May;170(5):2283–2286. [PMC free article] [PubMed]
  • Pattison-Granberg J, Persson BL. Regulation of cation-coupled high-affinity phosphate uptake in the yeast Saccharomyces cerevisiae. J Bacteriol. 2000 Sep;182(17):5017–5019. [PMC free article] [PubMed]
  • Patton-Vogt JL, Henry SA. GIT1, a gene encoding a novel transporter for glycerophosphoinositol in Saccharomyces cerevisiae. Genetics. 1998 Aug;149(4):1707–1715. [PMC free article] [PubMed]
  • Persson BL, Petersson J, Fristedt U, Weinander R, Berhe A, Pattison J. Phosphate permeases of Saccharomyces cerevisiae: structure, function and regulation. Biochim Biophys Acta. 1999 Nov 16;1422(3):255–272. [PubMed]
  • Theodoris G, Fong NM, Coons DM, Bisson LF. High-copy suppression of glucose transport defects by HXT4 and regulatory elements in the promoters of the HXT genes in Saccharomyces cerevisiae. Genetics. 1994 Aug;137(4):957–966. [PMC free article] [PubMed]
  • Schneider KR, Smith RL, O'Shea EK. Phosphate-regulated inactivation of the kinase PHO80-PHO85 by the CDK inhibitor PHO81. Science. 1994 Oct 7;266(5182):122–126. [PubMed]
  • Schwob E, Nasmyth K. CLB5 and CLB6, a new pair of B cyclins involved in DNA replication in Saccharomyces cerevisiae. Genes Dev. 1993 Jul;7(7A):1160–1175. [PubMed]
  • Wanner BL. Gene regulation by phosphate in enteric bacteria. J Cell Biochem. 1993 Jan;51(1):47–54. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...