• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntcellLink to Publisher's site
Plant Cell. May 1999; 11(5): 769–780.
PMCID: PMC144224

The irregular xylem3 locus of Arabidopsis encodes a cellulose synthase required for secondary cell wall synthesis.

Abstract

The irregular xylem3 (irx3) mutant of Arabidopsis has a severe deficiency in secondary cell wall cellulose deposition that leads to collapsed xylem cells. The irx3 mutation has been mapped to the top arm of chromosome V near the marker nga106. Expressed sequence tag clone 75G11, which exhibits sequence similarity to cellulose synthase, was found to be tightly linked to irx3, and genomic clones containing the gene corresponding to clone 75G11 complemented the irx3 mutation. Thus, the IRX3 gene encodes a cellulose synthase component that is specifically required for the synthesis of cellulose in the secondary cell wall. The irx3 mutant allele contains a stop codon that truncates the gene product by 168 amino acids, suggesting that this allele is null. Furthermore, in contrast to radial swelling1 (rsw1) plants, irx3 plants show no increase in the accumulation of beta-1,4-linked glucose in the noncrystalline cell wall fraction. IRX3 and RSW1 fall into a distinct subgroup (Csa) of Arabidopsis genes showing homology to bacterial cellulose synthases.

Full Text

The Full Text of this article is available as a PDF (383K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abler ML, Green PJ. Control of mRNA stability in higher plants. Plant Mol Biol. 1996 Oct;32(1-2):63–78. [PubMed]
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Arioli T, Peng L, Betzner AS, Burn J, Wittke W, Herth W, Camilleri C, Höfte H, Plazinski J, Birch R, et al. Molecular analysis of cellulose biosynthesis in Arabidopsis. Science. 1998 Jan 30;279(5351):717–720. [PubMed]
  • Brown MP, Aidoo KA, Vining LC. A role for pabAB, a p-aminobenzoate synthase gene of Streptomyces venezuelae ISP5230, in chloramphenicol biosynthesis. Microbiology. 1996 Jun;142(Pt 6):1345–1355. [PubMed]
  • Carpita N, Vergara C. A recipe for cellulose. Science. 1998 Jan 30;279(5351):672–673. [PubMed]
  • Creusot F, Fouilloux E, Dron M, Lafleuriel J, Picard G, Billault A, Le Paslier D, Cohen D, Chabouté ME, Durr A, et al. The CIC library: a large insert YAC library for genome mapping in Arabidopsis thaliana. Plant J. 1995 Nov;8(5):763–770. [PubMed]
  • Cutler S, Somerville C. Cloning in silico. Curr Biol. 1997 Feb 1;7(2):R108–R111. [PubMed]
  • Deckert G, Warren PV, Gaasterland T, Young WG, Lenox AL, Graham DE, Overbeek R, Snead MA, Keller M, Aujay M, et al. The complete genome of the hyperthermophilic bacterium Aquifex aeolicus. Nature. 1998 Mar 26;392(6674):353–358. [PubMed]
  • Delmer DP, Amor Y. Cellulose biosynthesis. Plant Cell. 1995 Jul;7(7):987–1000. [PMC free article] [PubMed]
  • Emons AM, Mulder BM. The making of the architecture of the plant cell wall: how cells exploit geometry. Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7215–7219. [PMC free article] [PubMed]
  • Giddings TH, Jr, Brower DL, Staehelin LA. Visualization of particle complexes in the plasma membrane of Micrasterias denticulata associated with the formation of cellulose fibrils in primary and secondary cell walls. J Cell Biol. 1980 Feb;84(2):327–339. [PMC free article] [PubMed]
  • Matthysse AG, White S, Lightfoot R. Genes required for cellulose synthesis in Agrobacterium tumefaciens. J Bacteriol. 1995 Feb;177(4):1069–1075. [PMC free article] [PubMed]
  • Meyer K, Leube MP, Grill E. A protein phosphatase 2C involved in ABA signal transduction in Arabidopsis thaliana. Science. 1994 Jun 3;264(5164):1452–1455. [PubMed]
  • O'Donnell PJ, Truesdale MR, Calvert CM, Dorans A, Roberts MR, Bowles DJ. A novel tomato gene that rapidly responds to wound- and pathogen-related signals. Plant J. 1998 Apr;14(1):137–142. [PubMed]
  • Pear JR, Kawagoe Y, Schreckengost WE, Delmer DP, Stalker DM. Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12637–12642. [PMC free article] [PubMed]
  • Saxena IM, Lin FC, Brown RM., Jr Cloning and sequencing of the cellulose synthase catalytic subunit gene of Acetobacter xylinum. Plant Mol Biol. 1990 Nov;15(5):673–683. [PubMed]
  • Saxena IM, Brown RM, Jr, Fevre M, Geremia RA, Henrissat B. Multidomain architecture of beta-glycosyl transferases: implications for mechanism of action. J Bacteriol. 1995 Mar;177(6):1419–1424. [PMC free article] [PubMed]
  • Schmidt R, Love K, West J, Lenehan Z, Dean C. Description of 31 YAC contigs spanning the majority of Arabidopsis thaliana chromosome 5. Plant J. 1997 Mar;11(3):563–572. [PubMed]
  • Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. [PMC free article] [PubMed]
  • Turner SR, Somerville CR. Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell. 1997 May;9(5):689–701. [PMC free article] [PubMed]
  • Voytas DF, Konieczny A, Cummings MP, Ausubel FM. The structure, distribution and evolution of the Ta1 retrotransposable element family of Arabidopsis thaliana. Genetics. 1990 Nov;126(3):713–721. [PMC free article] [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • Gene (nucleotide)
    Gene (nucleotide)
    Records in Gene identified from shared sequence links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Nucleotide
    Nucleotide
    Published Nucleotide sequences
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • Protein
    Protein
    Published protein sequences
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree