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Plant Cell. May 1998; 10(5): 765–779.
PMCID: PMC144027

Xa21D encodes a receptor-like molecule with a leucine-rich repeat domain that determines race-specific recognition and is subject to adaptive evolution.

Abstract

The rice Xa21 gene confers resistance to Xanthomonas oryzae pv oryzae in a race-specific manner. Analysis of the inheritance patterns and resistance spectra of transgenic plants carrying six Xa21 gene family members indicated that one member, designated Xa21D, displayed a resistance spectrum identical to that observed for Xa21 but conferred only partial resistance. Xa21D encodes a receptor-like protein carrying leucine-rich repeat (LRR) motifs in the presumed extracellular domain. The Xa21D transcript terminates shortly after the stop codon introduced by the retrotransposon Retrofit. Comparison of nucleotide substitutions in the LRR coding regions of Xa21 and Xa21D provided evidence of adaptive selection. Both functional and evolutionary evidence indicates that the Xa21D LRR domain controls race-specific pathogen recognition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Alleman M, Kermicle JL. Somatic variegation and germinal mutability reflect the position of transposable element Dissociation within the maize R gene. Genetics. 1993 Sep;135(1):189–203. [PMC free article] [PubMed]
  • Anderson PA, Lawrence GJ, Morrish BC, Ayliffe MA, Finnegan EJ, Ellis JG. Inactivation of the flax rust resistance gene M associated with loss of a repeated unit within the leucine-rich repeat coding region. Plant Cell. 1997 Apr;9(4):641–651. [PMC free article] [PubMed]
  • Becraft PW, Stinard PS, McCarty DR. CRINKLY4: A TNFR-like receptor kinase involved in maize epidermal differentiation. Science. 1996 Sep 6;273(5280):1406–1409. [PubMed]
  • Braun T, Schofield PR, Sprengel R. Amino-terminal leucine-rich repeats in gonadotropin receptors determine hormone selectivity. EMBO J. 1991 Jul;10(7):1885–1890. [PMC free article] [PubMed]
  • Bunkers G, Nelson OE, Jr, Raboy V. Maize bronze 1:dSpm insertion mutations that are not fully suppressed by an active Spm. Genetics. 1993 Aug;134(4):1211–1220. [PMC free article] [PubMed]
  • Chang C, Schaller GE, Patterson SE, Kwok SF, Meyerowitz EM, Bleecker AB. The TMK1 gene from Arabidopsis codes for a protein with structural and biochemical characteristics of a receptor protein kinase. Plant Cell. 1992 Oct;4(10):1263–1271. [PMC free article] [PubMed]
  • Clark SE, Williams RW, Meyerowitz EM. The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis. Cell. 1997 May 16;89(4):575–585. [PubMed]
  • Dixon MS, Jones DA, Keddie JS, Thomas CM, Harrison K, Jones JD. The tomato Cf-2 disease resistance locus comprises two functional genes encoding leucine-rich repeat proteins. Cell. 1996 Feb 9;84(3):451–459. [PubMed]
  • Endo T, Ikeo K, Gojobori T. Large-scale search for genes on which positive selection may operate. Mol Biol Evol. 1996 May;13(5):685–690. [PubMed]
  • Fantl WJ, Johnson DE, Williams LT. Signalling by receptor tyrosine kinases. Annu Rev Biochem. 1993;62:453–481. [PubMed]
  • Fridell RA, Pret AM, Searles LL. A retrotransposon 412 insertion within an exon of the Drosophila melanogaster vermilion gene is spliced from the precursor RNA. Genes Dev. 1990 Apr;4(4):559–566. [PubMed]
  • Gardina PJ, Manson MD. Attractant signaling by an aspartate chemoreceptor dimer with a single cytoplasmic domain. Science. 1996 Oct 18;274(5286):425–426. [PubMed]
  • Heldin CH, Ostman A. Ligand-induced dimerization of growth factor receptors: variations on the theme. Cytokine Growth Factor Rev. 1996 Jun;7(1):3–10. [PubMed]
  • Hiscock SJ, Kües U, Dickinson HG. Molecular mechanisms of self-incompatibility in flowering plants and fungi - different means to the same end. Trends Cell Biol. 1996 Nov;6(11):421–428. [PubMed]
  • Hughes AL, Nei M. Pattern of nucleotide substitution at major histocompatibility complex class I loci reveals overdominant selection. Nature. 1988 Sep 8;335(6186):167–170. [PubMed]
  • Hunter T. Protein kinases and phosphatases: the yin and yang of protein phosphorylation and signaling. Cell. 1995 Jan 27;80(2):225–236. [PubMed]
  • Kim HY, Schiefelbein JW, Raboy V, Furtek DB, Nelson OE., Jr RNA splicing permits expression of a maize gene with a defective Suppressor-mutator transposable element insertion in an exon. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5863–5867. [PMC free article] [PubMed]
  • Kobe B, Deisenhofer J. The leucine-rich repeat: a versatile binding motif. Trends Biochem Sci. 1994 Oct;19(10):415–421. [PubMed]
  • Leicht BG, Muse SV, Hanczyc M, Clark AG. Constraints on intron evolution in the gene encoding the myosin alkali light chain in Drosophila. Genetics. 1995 Jan;139(1):299–308. [PMC free article] [PubMed]
  • Li J, Chory J. A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell. 1997 Sep 5;90(5):929–938. [PubMed]
  • Liu Y, Alleman M, Wessler SR. A Ds insertion alters the nuclear localization of the maize transcriptional activator R. Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7816–7820. [PMC free article] [PubMed]
  • Massagué J. TGFbeta signaling: receptors, transducers, and Mad proteins. Cell. 1996 Jun 28;85(7):947–950. [PubMed]
  • Menssen A, Höhmann S, Martin W, Schnable PS, Peterson PA, Saedler H, Gierl A. The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene. EMBO J. 1990 Oct;9(10):3051–3057. [PMC free article] [PubMed]
  • Messier W, Stewart CB. Episodic adaptive evolution of primate lysozymes. Nature. 1997 Jan 9;385(6612):151–154. [PubMed]
  • Nasrallah JB, Stein JC, Kandasamy MK, Nasrallah ME. Signaling the arrest of pollen tube development in self-incompatible plants. Science. 1994 Dec 2;266(5190):1505–1508. [PubMed]
  • Nei M, Gojobori T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986 Sep;3(5):418–426. [PubMed]
  • Nelson RJ, Baraoidan MR, Cruz CM, Yap IV, Leach JE, Mew TW, Leung H. Relationship between Phylogeny and Pathotype for the Bacterial Blight Pathogen of Rice. Appl Environ Microbiol. 1994 Sep;60(9):3275–3283. [PMC free article] [PubMed]
  • Parniske M, Hammond-Kosack KE, Golstein C, Thomas CM, Jones DA, Harrison K, Wulff BB, Jones JD. Novel disease resistance specificities result from sequence exchange between tandemly repeated genes at the Cf-4/9 locus of tomato. Cell. 1997 Dec 12;91(6):821–832. [PubMed]
  • Pawson T. Protein modules and signalling networks. Nature. 1995 Feb 16;373(6515):573–580. [PubMed]
  • Raboy V, Kim HY, Schiefelbein JW, Nelson-Jr OE. Deletions in a dspm insert in a maize bronze-1 allele alter RNA processing and gene expression. Genetics. 1989 Jul;122(3):695–703. [PMC free article] [PubMed]
  • Ronald PC. The molecular basis of disease resistance in rice. Plant Mol Biol. 1997 Sep;35(1-2):179–186. [PubMed]
  • Ronald PC, Albano B, Tabien R, Abenes L, Wu KS, McCouch S, Tanksley SD. Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21. Mol Gen Genet. 1992 Dec;236(1):113–120. [PubMed]
  • Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW, Staskawicz BJ. Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato. Science. 1996 Dec 20;274(5295):2063–2065. [PubMed]
  • Song WY, Wang GL, Chen LL, Kim HS, Pi LY, Holsten T, Gardner J, Wang B, Zhai WX, Zhu LH, et al. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science. 1995 Dec 15;270(5243):1804–1806. [PubMed]
  • Song WY, Pi LY, Wang GL, Gardner J, Holsten T, Ronald PC. Evolution of the rice Xa21 disease resistance gene family. Plant Cell. 1997 Aug;9(8):1279–1287. [PMC free article] [PubMed]
  • Staskawicz BJ, Ausubel FM, Baker BJ, Ellis JG, Jones JD. Molecular genetics of plant disease resistance. Science. 1995 May 5;268(5211):661–667. [PubMed]
  • Stein JC, Dixit R, Nasrallah ME, Nasrallah JB. SRK, the stigma-specific S locus receptor kinase of Brassica, is targeted to the plasma membrane in transgenic tobacco. Plant Cell. 1996 Mar;8(3):429–445. [PMC free article] [PubMed]
  • Tang X, Frederick RD, Zhou J, Halterman DA, Jia Y, Martin GB. Initiation of Plant Disease Resistance by Physical Interaction of AvrPto and Pto Kinase. Science. 1996 Dec 20;274(5295):2060–2063. [PubMed]
  • Tatsuno I, Homma M, Oosawa K, Kawagishi I. Signaling by the Escherichia coli aspartate chemoreceptor Tar with a single cytoplasmic domain per dimer. Science. 1996 Oct 18;274(5286):423–425. [PubMed]
  • ten Dijke P, Miyazono K, Heldin CH. Signaling via hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. Curr Opin Cell Biol. 1996 Apr;8(2):139–145. [PubMed]
  • Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. [PMC free article] [PubMed]
  • Torii KU, Mitsukawa N, Oosumi T, Matsuura Y, Yokoyama R, Whittier RF, Komeda Y. The Arabidopsis ERECTA gene encodes a putative receptor protein kinase with extracellular leucine-rich repeats. Plant Cell. 1996 Apr;8(4):735–746. [PMC free article] [PubMed]
  • Ullrich A, Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell. 1990 Apr 20;61(2):203–212. [PubMed]
  • Valon C, Smalle J, Goodman HM, Giraudat J. Characterization of an Arabidopsis thaliana gene (TMKL1) encoding a putative transmembrane protein with an unusual kinase-like domain. Plant Mol Biol. 1993 Oct;23(2):415–421. [PubMed]
  • Van den Ackerveken G, Marois E, Bonas U. Recognition of the bacterial avirulence protein AvrBs3 occurs inside the host plant cell. Cell. 1996 Dec 27;87(7):1307–1316. [PubMed]
  • Walker JC. Structure and function of the receptor-like protein kinases of higher plants. Plant Mol Biol. 1994 Dec;26(5):1599–1609. [PubMed]
  • Wang GL, Song WY, Ruan DL, Sideris S, Ronald PC. The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants. Mol Plant Microbe Interact. 1996 Dec;9(9):850–855. [PubMed]
  • Wessler SR. The splicing of maize transposable elements from pre-mRNA--a minireview. Gene. 1989 Oct 15;82(1):127–133. [PubMed]
  • Williams CE, Ronald PC. PCR template-DNA isolated quickly from monocot and dicot leaves without tissue homogenization. Nucleic Acids Res. 1994 May 25;22(10):1917–1918. [PMC free article] [PubMed]
  • Wolfe KH, Gouy M, Yang YW, Sharp PM, Li WH. Date of the monocot-dicot divergence estimated from chloroplast DNA sequence data. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6201–6205. [PMC free article] [PubMed]
  • Zhang J, Kumar S, Nei M. Small-sample tests of episodic adaptive evolution: a case study of primate lysozymes. Mol Biol Evol. 1997 Dec;14(12):1335–1338. [PubMed]

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