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Plant Cell. Feb 1998; 10(2): 171–182.
PMCID: PMC143982

Multiple AGAMOUS homologs from cucumber and petunia differ in their ability to induce reproductive organ fate.

Abstract

The C function in Arabidopsis, which specifies stamen and carpel identity, is represented by a single gene called AGAMOUS (AG). From both petunia and cucumber, two MADS box genes have been isolated. Both share a high degree of amino acid sequence identity with the Arabidopsis AG protein. Their roles in specifying stamen and carpel identity have been studied by ectopic expression in petunia, resulting in plants with different floral phenotypes. Cucumber MADS box gene 1 (CUM1) induced severe homeotic transformations of sepals into carpelloid structures and petals into stamens, which is similar to ectopic AG expression in Arabidopsis plants. Overexpression of the other cucumber AG homolog, CUM10, resulted in plants with partial transformations of the petals into antheroid structures, indicating that CUM10 is also able to promote floral organ identity. From the two petunia AG homologs pMADS3 and Floral Binding Protein gene 6 (FBP6), only pMADS3 was able to induce homeotic transformations of sepals and petals. Ectopic expression of both pMADS3 and FBP6, as occurrs in the petunia homeotic mutant blind, phenocopies the pMADS3 single overexpresser plants, indicating that there is no additive effect of concerted expression. This study demonstrates that in petunia and cucumber, multiple AG homologs exist, although they differ in their ability to induce reproductive organ fate.

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Selected References

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  • Angenent GC, Busscher M, Franken J, Mol JN, van Tunen AJ. Differential expression of two MADS box genes in wild-type and mutant petunia flowers. Plant Cell. 1992 Aug;4(8):983–993. [PMC free article] [PubMed]
  • Angenent GC, Franken J, Busscher M, Colombo L, van Tunen AJ. Petal and stamen formation in petunia is regulated by the homeotic gene fbp1. Plant J. 1993 Jul;4(1):101–112. [PubMed]
  • Angenent GC, Franken J, Busscher M, van Dijken A, van Went JL, Dons HJ, van Tunen AJ. A novel class of MADS box genes is involved in ovule development in petunia. Plant Cell. 1995 Oct;7(10):1569–1582. [PMC free article] [PubMed]
  • Bowman JL, Smyth DR, Meyerowitz EM. Genetic interactions among floral homeotic genes of Arabidopsis. Development. 1991 May;112(1):1–20. [PubMed]
  • Bradley D, Carpenter R, Sommer H, Hartley N, Coen E. Complementary floral homeotic phenotypes result from opposite orientations of a transposon at the plena locus of Antirrhinum. Cell. 1993 Jan 15;72(1):85–95. [PubMed]
  • Carpenter R, Coen ES. Floral homeotic mutations produced by transposon-mutagenesis in Antirrhinum majus. Genes Dev. 1990 Sep;4(9):1483–1493. [PubMed]
  • Coen ES, Meyerowitz EM. The war of the whorls: genetic interactions controlling flower development. Nature. 1991 Sep 5;353(6339):31–37. [PubMed]
  • Colombo L, Franken J, Koetje E, van Went J, Dons HJ, Angenent GC, van Tunen AJ. The petunia MADS box gene FBP11 determines ovule identity. Plant Cell. 1995 Nov;7(11):1859–1868. [PMC free article] [PubMed]
  • Drews GN, Bowman JL, Meyerowitz EM. Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell. 1991 Jun 14;65(6):991–1002. [PubMed]
  • Feinberg AP, Vogelstein B. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984 Feb;137(1):266–267. [PubMed]
  • Florack DE, Dirkse WG, Visser B, Heidekamp F, Stiekema WJ. Expression of biologically active hordothionins in tobacco. Effects of pre- and pro-sequences at the amino and carboxyl termini of the hordothionin precursor on mature protein expression and sorting. Plant Mol Biol. 1994 Jan;24(1):83–96. [PubMed]
  • Huang H, Mizukami Y, Hu Y, Ma H. Isolation and characterization of the binding sequences for the product of the Arabidopsis floral homeotic gene AGAMOUS. Nucleic Acids Res. 1993 Oct 11;21(20):4769–4776. [PMC free article] [PubMed]
  • Kang HG, Noh YS, Chung YY, Costa MA, An K, An G. Phenotypic alterations of petal and sepal by ectopic expression of a rice MADS box gene in tobacco. Plant Mol Biol. 1995 Oct;29(1):1–10. [PubMed]
  • Kempin SA, Mandel MA, Yanofsky MF. Conversion of perianth into reproductive organs by ectopic expression of the tobacco floral homeotic gene NAG1. Plant Physiol. 1993 Dec;103(4):1041–1046. [PMC free article] [PubMed]
  • Koes R, Souer E, van Houwelingen A, Mur L, Spelt C, Quattrocchio F, Wing J, Oppedijk B, Ahmed S, Maes T, et al. Targeted gene inactivation in petunia by PCR-based selection of transposon insertion mutants. Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8149–8153. [PMC free article] [PubMed]
  • Ma H, Yanofsky MF, Meyerowitz EM. AGL1-AGL6, an Arabidopsis gene family with similarity to floral homeotic and transcription factor genes. Genes Dev. 1991 Mar;5(3):484–495. [PubMed]
  • Mandel MA, Bowman JL, Kempin SA, Ma H, Meyerowitz EM, Yanofsky MF. Manipulation of flower structure in transgenic tobacco. Cell. 1992 Oct 2;71(1):133–143. [PubMed]
  • Mena M, Ambrose BA, Meeley RB, Briggs SP, Yanofsky MF, Schmidt RJ. Diversification of C-function activity in maize flower development. Science. 1996 Nov 29;274(5292):1537–1540. [PubMed]
  • Mizukami Y, Ma H. Ectopic expression of the floral homeotic gene AGAMOUS in transgenic Arabidopsis plants alters floral organ identity. Cell. 1992 Oct 2;71(1):119–131. [PubMed]
  • Mizukami Y, Huang H, Tudor M, Hu Y, Ma H. Functional domains of the floral regulator AGAMOUS: characterization of the DNA binding domain and analysis of dominant negative mutations. Plant Cell. 1996 May;8(5):831–845. [PMC free article] [PubMed]
  • Pnueli L, Abu-Abeid M, Zamir D, Nacken W, Schwarz-Sommer Z, Lifschitz E. The MADS box gene family in tomato: temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis. Plant J. 1991 Sep;1(2):255–266. [PubMed]
  • Pnueli L, Hareven D, Rounsley SD, Yanofsky MF, Lifschitz E. Isolation of the tomato AGAMOUS gene TAG1 and analysis of its homeotic role in transgenic plants. Plant Cell. 1994 Feb;6(2):163–173. [PMC free article] [PubMed]
  • Pollock R, Treisman R. Human SRF-related proteins: DNA-binding properties and potential regulatory targets. Genes Dev. 1991 Dec;5(12A):2327–2341. [PubMed]
  • Purugganan MD, Rounsley SD, Schmidt RJ, Yanofsky MF. Molecular evolution of flower development: diversification of the plant MADS-box regulatory gene family. Genetics. 1995 May;140(1):345–356. [PMC free article] [PubMed]
  • Saedler H, Huijser P. Molecular biology of flower development in Antirrhinum majus (snapdragon). Gene. 1993 Dec 15;135(1-2):239–243. [PubMed]
  • Savidge B, Rounsley SD, Yanofsky MF. Temporal relationship between the transcription of two Arabidopsis MADS box genes and the floral organ identity genes. Plant Cell. 1995 Jun;7(6):721–733. [PMC free article] [PubMed]
  • Schwarz-Sommer Z, Huijser P, Nacken W, Saedler H, Sommer H. Genetic Control of Flower Development by Homeotic Genes in Antirrhinum majus. Science. 1990 Nov 16;250(4983):931–936. [PubMed]
  • Tsuchimoto S, van der Krol AR, Chua NH. Ectopic expression of pMADS3 in transgenic petunia phenocopies the petunia blind mutant. Plant Cell. 1993 Aug;5(8):843–853. [PMC free article] [PubMed]
  • Verwoerd TC, Dekker BM, Hoekema A. A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 1989 Mar 25;17(6):2362–2362. [PMC free article] [PubMed]
  • Weigel D, Meyerowitz EM. The ABCs of floral homeotic genes. Cell. 1994 Jul 29;78(2):203–209. [PubMed]
  • Yanofsky MF, Ma H, Bowman JL, Drews GN, Feldmann KA, Meyerowitz EM. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors. Nature. 1990 Jul 5;346(6279):35–39. [PubMed]

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