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Plant Cell. Dec 1998; 10(12): 2047–2062.
PMCID: PMC143977

GLABROUS1 overexpression and TRIPTYCHON alter the cell cycle and trichome cell fate in Arabidopsis.


Cellular competence, initiation cues, and inhibition signals control the distribution of trichomes on the Arabidopsis leaf. The GLABROUS1 (GL1) gene has a dual role in that it is required for trichome initiation, but GL1 overexpression reduces trichome number. We have found that a mutation in the TRIPTYCHON (TRY) gene partially suppresses the GL1 overexpression phenotype but not in a way that indicates that TRY directly controls an epidermal inhibition pathway. Surprisingly, cauliflower mosaic virus 35S::GL1 try plants contain a subclass of trichomes derived from the subepidermal layer. Altered cell cycle control was also detected in 35S::GL1 and try plants. A mutation in TRY led to increased epidermal and mesophyll cell number, a reduction in endoreduplication in the epidermis, and an increase in endoreduplication in trichomes. GL1 overexpression also reduced endoreduplication levels in both the epidermis and trichomes; however, in the presence of try, it synergistically enhanced trichome endoreduplication. Interactions with the COTYLEDON TRICHOME1 (COT1) gene indicate that GL1 and TRY control trichome development and may be involved in cell cycle control during leaf development.

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Selected References

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  • Andrews B, Measday V. The cyclin family of budding yeast: abundant use of a good idea. Trends Genet. 1998 Feb;14(2):66–72. [PubMed]
  • Brugarolas J, Bronson RT, Jacks T. p21 is a critical CDK2 regulator essential for proliferation control in Rb-deficient cells. J Cell Biol. 1998 Apr 20;141(2):503–514. [PMC free article] [PubMed]
  • Folkers U, Berger J, Hülskamp M. Cell morphogenesis of trichomes in Arabidopsis: differential control of primary and secondary branching by branch initiation regulators and cell growth. Development. 1997 Oct;124(19):3779–3786. [PubMed]
  • Galbraith DW, Harkins KR, Knapp S. Systemic Endopolyploidy in Arabidopsis thaliana. Plant Physiol. 1991 Jul;96(3):985–989. [PMC free article] [PubMed]
  • Hemerly A, Engler J de A, Bergounioux C, Van Montagu M, Engler G, Inzé D, Ferreira P. Dominant negative mutants of the Cdc2 kinase uncouple cell division from iterative plant development. EMBO J. 1995 Aug 15;14(16):3925–3936. [PMC free article] [PubMed]
  • Hülskamp M, Misŕa S, Jürgens G. Genetic dissection of trichome cell development in Arabidopsis. Cell. 1994 Feb 11;76(3):555–566. [PubMed]
  • Koornneef M, Dellaert LW, van der Veen JH. EMS- and radiation-induced mutation frequencies at individual loci in Arabidopsis thaliana (L.) Heynh. Mutat Res. 1982 Mar;93(1):109–123. [PubMed]
  • Larkin JC, Oppenheimer DG, Pollock S, Marks MD. Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for Function. Plant Cell. 1993 Dec;5(12):1739–1748. [PMC free article] [PubMed]
  • Larkin JC, Oppenheimer DG, Lloyd AM, Paparozzi ET, Marks MD. Roles of the GLABROUS1 and TRANSPARENT TESTA GLABRA Genes in Arabidopsis Trichome Development. Plant Cell. 1994 Aug;6(8):1065–1076. [PMC free article] [PubMed]
  • Larkin JC, Young N, Prigge M, Marks MD. The control of trichome spacing and number in Arabidopsis. Development. 1996 Mar;122(3):997–1005. [PubMed]
  • Larkin JC, Marks MD, Nadeau J, Sack F. Epidermal cell fate and patterning in leaves. Plant Cell. 1997 Jul;9(7):1109–1120. [PMC free article] [PubMed]
  • Lloyd AM, Schena M, Walbot V, Davis RW. Epidermal cell fate determination in Arabidopsis: patterns defined by a steroid-inducible regulator. Science. 1994 Oct 21;266(5184):436–439. [PubMed]
  • Marks MD. Plant development. The making of a plant hair. Curr Biol. 1994 Jul 1;4(7):621–623. [PubMed]
  • Marks M David. MOLECULAR GENETIC ANALYSIS OF TRICHOME DEVELOPMENT IN ARABIDOPSIS. Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48(NaN):137–163. [PubMed]
  • Melaragno JE, Mehrotra B, Coleman AW. Relationship between Endopolyploidy and Cell Size in Epidermal Tissue of Arabidopsis. Plant Cell. 1993 Nov;5(11):1661–1668. [PMC free article] [PubMed]
  • Nasmyth K. Control of the yeast cell cycle by the Cdc28 protein kinase. Curr Opin Cell Biol. 1993 Apr;5(2):166–179. [PubMed]
  • Oppenheimer DG, Herman PL, Sivakumaran S, Esch J, Marks MD. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell. 1991 Nov 1;67(3):483–493. [PubMed]
  • Schnittger A, Jürgens G, Hülskamp M. Tissue layer and organ specificity of trichome formation are regulated by GLABRA1 and TRIPTYCHON in Arabidopsis. Development. 1998 Jun;125(12):2283–2289. [PubMed]
  • Szymanski DB, Jilk RA, Pollock SM, Marks MD. Control of GL2 expression in Arabidopsis leaves and trichomes. Development. 1998 Apr;125(7):1161–1171. [PubMed]
  • Szymanski DB, Klis DA, Larkin JC, Marks MD. cot1: a regulator of Arabidopsis trichome initiation. Genetics. 1998 Jun;149(2):565–577. [PMC free article] [PubMed]

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