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Plant Cell. Dec 1998; 10(12): 2019–2032.
PMCID: PMC143976

Guidance in vitro of the pollen tube to the naked embryo sac of torenia fournieri

Abstract

The precise guidance of the pollen tube to the embryo sac is critical to the successful sexual reproduction of flowering plants. We demonstrate here the guidance of the pollen tube to the embryo sac in vitro by using the naked embryo sac of Torenia fournieri, which protrudes from the micropyle of the ovule. We developed a medium for culture of both the ovule and the pollen tube of T. fournieri and cocultivated them in a thin layer of solid medium. Although pollen tubes that had germinated in vitro passed naked embryo sacs, some pollen tubes that grew semi-in vitro through a cut style arrived precisely at the site of entry into the embryo sac, namely, the filiform apparatus of the synergids. When pollen tubes were unable to enter the embryo sac, they continuously grew toward the same filiform apparatus, forming narrow coils. Pollen tubes selectively arrived at complete, unfertilized embryo sacs but did not arrive at those of heat-treated ovules or those with disrupted synergids. These results convincingly demonstrate that pollen tubes are specifically attracted to the region of the filiform apparatus of living synergids in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cheung AY, Wang H, Wu HM. A floral transmitting tissue-specific glycoprotein attracts pollen tubes and stimulates their growth. Cell. 1995 Aug 11;82(3):383–393. [PubMed]
  • Hulskamp M, Schneitz K, Pruitt RE. Genetic Evidence for a Long-Range Activity That Directs Pollen Tube Guidance in Arabidopsis. Plant Cell. 1995 Jan;7(1):57–64. [PMC free article] [PubMed]
  • Kranz E, von Wiegen P, Quader H, Lorz H. Endosperm development after fusion of isolated, single maize sperm and central cells in vitro . Plant Cell. 1998 Apr;10(4):511–524. [PMC free article] [PubMed]
  • Lord EM, Sanders LC. Roles for the extracellular matrix in plant development and pollination: a special case of cell movement in plants. Dev Biol. 1992 Sep;153(1):16–28. [PubMed]
  • Malho R, Trewavas AJ. Localized Apical Increases of Cytosolic Free Calcium Control Pollen Tube Orientation. Plant Cell. 1996 Nov;8(11):1935–1949. [PMC free article] [PubMed]
  • Mascarenhas JP, Machlis L. Chemotropic Response of the Pollen of Antirrhinum majus to Calcium. Plant Physiol. 1964 Jan;39(1):70–77. [PMC free article] [PubMed]
  • Ray SM, Park SS, Ray A. Pollen tube guidance by the female gametophyte. Development. 1997 Jun;124(12):2489–2498. [PubMed]
  • Wilhelmi LK, Preuss D. Self-sterility in Arabidopsis due to defective pollen tube guidance. Science. 1996 Nov 29;274(5292):1535–1537. [PubMed]
  • Wilhelmi LK, Preuss D. Blazing New Trails (Pollen Tube Guidance in Flowering Plants). Plant Physiol. 1997 Feb;113(2):307–312. [PMC free article] [PubMed]
  • Wolters-Arts M, Lush WM, Mariani C. Lipids are required for directional pollen-tube growth. Nature. 1998 Apr 23;392(6678):818–821. [PubMed]
  • Wu HM, Wang H, Cheung AY. A pollen tube growth stimulatory glycoprotein is deglycosylated by pollen tubes and displays a glycosylation gradient in the flower. Cell. 1995 Aug 11;82(3):395–403. [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists

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