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Biophys J. 1984 Dec; 46(6): 821–825.
PMCID: PMC1435102

Calcium- and voltage-activated potassium channels in human macrophages.


Single calcium-activated potassium channel currents were recorded in intact and excised membrane patches from cultured human macrophages. Channel conductance was 240 pS in symmetrical 145 mM K+ and 130 pS in 5 mM external K+. Lower conductance current fluctuations (40% of the larger channels) with the same reversal potential as the higher conductance channels were noted in some patches. Ion substitution experiments indicated that the channel is permeable to potassium and relatively impermeable to sodium. The frequency of channel opening increased with depolarization and intracellular calcium concentration. At 10(-7) M (Ca++)i, channel activity was evident only at potentials of +40 mV or more depolarized, while at 10(-5) M, channels were open at all voltages tested (-40 to +60 mV). In intact patches, channels were seen at depolarized patch potentials of +50 mV or greater, indicating that the ionized calcium concentration in the macrophage is probably less than 10(-7) M.

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Selected References

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  • Barrett JN, Magleby KL, Pallotta BS. Properties of single calcium-activated potassium channels in cultured rat muscle. J Physiol. 1982 Oct;331:211–230. [PMC free article] [PubMed]
  • Gallin EK. Voltage clamp studies in macrophages from mouse spleen cultures. Science. 1981 Oct 23;214(4519):458–460. [PubMed]
  • Gallin EK. Electrophysiological properties of macrophages. Fed Proc. 1984 Jun;43(9):2385–2389. [PubMed]
  • Gallin EK, Wiederhold ML, Lipsky PE, Rosenthal AS. Spontaneous and induced membrane hyperpolarizations in macrophages. J Cell Physiol. 1975 Dec;86 (Suppl 2)(3 Pt 2):653–661. [PubMed]
  • Kolb HA, Schwarze W. Properties of a cation channel of large unit conductance in lymphocytes, macrophages and cultured muscle cells. Biophys J. 1984 Jan;45(1):136–138. [PMC free article] [PubMed]
  • Marty A. Ca-dependent K channels with large unitary conductance in chromaffin cell membranes. Nature. 1981 Jun 11;291(5815):497–500. [PubMed]
  • McCann FV, Cole JJ, Guyre PM, Russell JA. Action potentials in macrophages derived from human monocytes. Science. 1983 Feb 25;219(4587):991–993. [PubMed]
  • Persechini PM, Araujo EG, Oliveira-Castro GM. Electrophysiology of phagocytic membranes: induction of slow membrane hyperpolarizations in macrophages and macrophage polykaryons by intracellular calcium injection. J Membr Biol. 1981;61(2):81–90. [PubMed]
  • Petersen OH, Maruyama Y. Calcium-activated potassium channels and their role in secretion. Nature. 1984 Feb 23;307(5953):693–696. [PubMed]
  • Rosenstreich DL, Farrar JJ, Dougherty S. Absolute macrophage dependency of T lymphocyte activation by mitogens. J Immunol. 1976 Jan;116(1):131–139. [PubMed]
  • Singer JJ, Walsh JV. Large conductance ca-activated k channels in smooth muscle cell membrane: reduction in unitary currents due to internal na ions. Biophys J. 1984 Jan;45(1):68–70. [PMC free article] [PubMed]

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