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Gut. 1986 Jun; 27(6): 698–704.
PMCID: PMC1433329

Incidence of methanogenic bacteria in a sigmoidoscopy population: an association of methanogenic bacteria and diverticulosis.


This study determined the incidence and concentration of methane-producing bacteria in tap water enema samples of 130 individuals taken before sigmoidoscopy. The number of subjects classified in five major colonic groups were as follows: normal colon 36, diverticulosis 57, inflammatory bowel disease 11, colon polyps 34, and colon cancer 11. Some patients were placed in more than one category. Ninety four of the subjects or 72% had methanogenic bacteria ranging in concentration from 6 to about 3 X 10(10)/g dry weight of faeces. The predominant methanogen in all groups was Methanobrevibacter smithii. Chi-square analysis showed that the incidence of methanogens in concentrations of 10(7)/g dry weight of faeces or greater in patients with diverticulosis (58%) was significantly greater than in normal patients (25%). High methanogen concentrations are associated with excretion of methane in the breath.

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Selected References

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  • Bond JH, Jr, Engel RR, Levitt MD. Factors influencing pulmonary methane excretion in man. An indirect method of studying the in situ metabolism of the methane-producing colonic bacteria. J Exp Med. 1971 Mar 1;133(3):572–588. [PMC free article] [PubMed]
  • Miller TL, Wolin MJ, Conway de Macario E, Macario AJ. Isolation of Methanobrevibacter smithii from human feces. Appl Environ Microbiol. 1982 Jan;43(1):227–232. [PMC free article] [PubMed]
  • Miller TL, Wolin MJ. Enumeration of Methanobrevibacter smithii in human feces. Arch Microbiol. 1982 Feb;131(1):14–18. [PubMed]
  • Miller TL, Wolin MJ. Stability of Methanobrevibacter smithii populations in the microbial flora excreted from the human large bowel. Appl Environ Microbiol. 1983 Jan;45(1):317–318. [PMC free article] [PubMed]
  • McKay LF, Brydon WG, Eastwood MA, Smith JH. The influence of pentose on breath methane. Am J Clin Nutr. 1981 Dec;34(12):2728–2733. [PubMed]
  • Bjørneklett A, Jenssen E. Relationships between hydrogen (H2) and methane (CH4) production in man. Scand J Gastroenterol. 1982 Nov;17(8):985–992. [PubMed]
  • Pitt P, de Bruijn KM, Beeching MF, Goldberg E, Blendis LM. Studies on breath methane: the effect of ethnic origins and lactulose. Gut. 1980 Nov;21(11):951–954. [PMC free article] [PubMed]
  • Piqué JM, Pallarés M, Cusó E, Vilar-Bonet J, Gassull MA. Methane production and colon cancer. Gastroenterology. 1984 Sep;87(3):601–605. [PubMed]
  • McKay LF, Eastwood MA, Brydon WG. Methane excretion in man--a study of breath, flatus, and faeces. Gut. 1985 Jan;26(1):69–74. [PMC free article] [PubMed]
  • Tadesse K, Eastwood MA. Metabolism of dietary fibre components in man assessed by breath hydrogen and methane. Br J Nutr. 1978 Sep;40(2):393–396. [PubMed]
  • Beaven J, Bjørneklett A, Jenssen E, Blomhoff JP, Skrede S. Pulmonary hydrogen and methane and plasma ammonia after the administration of lactulose or sorbitol. Scand J Gastroenterol. 1983 May;18(3):343–347. [PubMed]
  • Marthinsen D, Fleming SE. Excretion of breath and flatus gases by humans consuming high-fiber diets. J Nutr. 1982 Jun;112(6):1133–1143. [PubMed]
  • Haines A, Metz G, Dilawari J, Blendis L, Wiggins H. Breath-methane in patients with cancer of the large bowel. Lancet. 1977 Sep 3;2(8036):481–483. [PubMed]
  • Karlin DA, Jones RD, Stroehlein JR, Mastromarino AJ, Potter GD. Breath methane excretion in patients with unresected colorectal cancer. J Natl Cancer Inst. 1982 Sep;69(3):573–576. [PubMed]
  • Metz G, Gassull MA, Leeds AR, Blendis LM, Jenkins DJ. A simple method of measuring breath hydrogen in carbohydrate malabsorption by end-expiratory sampling. Clin Sci Mol Med. 1976 Mar;50(3):237–240. [PubMed]
  • Niu HC, Schoeller DA, Klein PD. Improved gas chromatographic quantitation of breath hydrogen by normalization to respiratory carbon dioxide. J Lab Clin Med. 1979 Nov;94(5):755–763. [PubMed]
  • Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. [PMC free article] [PubMed]
  • Miller TL, Weaver GA, Wolin MJ. Methanogens and anaerobes in a colon segment isolated from the normal fecal stream. Appl Environ Microbiol. 1984 Aug;48(2):449–450. [PMC free article] [PubMed]
  • Wolin MJ. Fermentation in the rumen and human large intestine. Science. 1981 Sep 25;213(4515):1463–1468. [PubMed]
  • Daniels L, Sparling R, Sprott GD. The bioenergetics of methanogenesis. Biochim Biophys Acta. 1984 Sep 6;768(2):113–163. [PubMed]

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