Logo of immunologyLink to Publisher's site
Immunology. Sep 1994; 83(1): 140–147.
PMCID: PMC1415006

Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1.


The monoclonal antibody (mAb) ED1 is being used widely as a marker for rat macrophages. The distribution of the recognized antigen in tissues and isolated cells strongly supports this use as a macrophage marker, since the majority of macrophages are recognized and only seldomly are other cell types stained by mAb ED1. In the present study we further characterized the recognized antigen by a detailed description of the localization of the antigen and by determining biochemical and functional properties. We show that the antigen is expressed on the membranes of cytoplasmic granules, like phagolysosomes, as well as on the cell surface. The amount of ED1 expression in a single cell can be correlated to phagocytic activity of the respective cell type, but the mAb ED1 is not able to block latex phagocytosis or bacterial killing. The mAb ED1 appears to recognize a heavily glycosylated protein of 90,000-110,000 MW, depending on the cell type used as antigen source. A possible relation with other known lysosomal glycoproteins with a similar molecular weight is discussed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Dijkstra CD, Döpp EA, Joling P, Kraal G. The heterogeneity of mononuclear phagocytes in lymphoid organs: distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1, ED2 and ED3. Immunology. 1985 Mar;54(3):589–599. [PMC free article] [PubMed]
  • Damoiseaux JG, Döpp EA, Neefjes JJ, Beelen RH, Dijkstra CD. Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18). J Leukoc Biol. 1989 Dec;46(6):556–564. [PubMed]
  • Damoiseaux JG, Döpp EA, Beelen RH, Dijkstra CD. Rat bone marrow and monocyte cultures: influence of culture time and lymphokines on the expression of macrophage differentiation antigens. J Leukoc Biol. 1989 Sep;46(3):246–253. [PubMed]
  • Honda H, Kimura H, Rostami A. Demonstration and phenotypic characterization of resident macrophages in rat skeletal muscle. Immunology. 1990 Jun;70(2):272–277. [PMC free article] [PubMed]
  • Steinman RM, Mellman IS, Muller WA, Cohn ZA. Endocytosis and the recycling of plasma membrane. J Cell Biol. 1983 Jan;96(1):1–27. [PMC free article] [PubMed]
  • Peters JH, Ruhl S, Friedrichs D. Veiled accessory cells deduced from monocytes. Immunobiology. 1987 Dec;176(1-2):154–166. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Beschorner WE, Armas OA. Loss of medullary dendritic cells in the thymus after cyclosporine and irradiation. Cell Immunol. 1991 Feb;132(2):505–514. [PubMed]
  • van Oostveen DC, van den Berg TK, Damoiseaux JG, van Rees EP. Macrophage subpopulations and reticulum cells in rat placenta. An immunohistochemical study. Cell Tissue Res. 1992 Jun;268(3):513–519. [PubMed]
  • Yamamoto K, Ikehara Y, Kawamoto S, Kato K. Characterization of enzymes and glycoproteins in rat liver lysosomal membranes. J Biochem. 1980 Jan;87(1):237–248. [PubMed]
  • Burnside J, Schneider DL. Characterization of the membrane proteins of rat liver lysosomes. Composition, enzyme activities and turnover. Biochem J. 1982 May 15;204(2):525–534. [PMC free article] [PubMed]
  • Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985 Jul;101(1):85–95. [PMC free article] [PubMed]
  • Chen JW, Cha Y, Yuksel KU, Gracy RW, August JT. Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. Sequence similarity to proteins bearing onco-differentiation antigens. J Biol Chem. 1988 Jun 25;263(18):8754–8758. [PubMed]
  • Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT. Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360–378. [PubMed]
  • Rabinowitz SS, Gordon S. Macrosialin, a macrophage-restricted membrane sialoprotein differentially glycosylated in response to inflammatory stimuli. J Exp Med. 1991 Oct 1;174(4):827–836. [PMC free article] [PubMed]
  • Matsuno K, Fujii H, Kotani M. Splenic marginal-zone macrophages and marginal metallophils in rats and mice. Cell Tissue Res. 1986;246(2):263–269. [PubMed]
  • Duijvestijn AM, Köhler YG, Hoefsmit EC. Interdigitating cells and macrophages in the acute involuting rat thymus. An electron-microscopic study on phagocytic activity and population development. Cell Tissue Res. 1982;224(2):291–301. [PubMed]
  • Fossum S, Rolstad B, Ford WL. Thymus independence, kinetics and phagocytic ability of interdigitating cells. Immunobiology. 1984 Dec;168(3-5):403–413. [PubMed]
  • Leijh PC, van den Barselaar MT, van Furth R. Kinetics of phagocytosis and intracellular killing of Staphylococcus aureus and Escherichia coli by human monocytes. Scand J Immunol. 1981;13(2):159–174. [PubMed]

Articles from Immunology are provided here courtesy of British Society for Immunology


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...