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Published online Jan 24, 2006. doi: 10.1186/1472-6750-6-7

Table 3

Comparison of knockdown efficacy and siRNA design algorithm. Average knockdown was measured by real-time PCR of triplicate samples. All averages are accurate within 10% SEM. Asterisks indicate high Takasaki et al. algorithm scores that have poor corresponding knockdown efficacy.

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nomenclaturecell tested% knockdown5' ΔΔGReynoldsHsiehAmarzguiouiUi-TeiTakasaki

shASC-721THP1910.6560228.07
shASC-743THP174-2.8652427.33
shCLR16.2-482jurkat37-1.42534310.1 *
shCLR16.2-716jurkat64-1.6860435.05
shCLR16.2-3394jurkat741.0692340
shCLR16.2-1630jurkat59-8.181544.81
shCLR19.3-667THP171-1.59311110.1
shCLR19.3-991THP113-4.62523310.1 *
shCLR19.3-1504THP1701.0640008.42
shCLR19.6-888jurkat<10%-0.3352223.78
shCLR19.6-1549jurkat<10%-2.135121-2.6
shCLR19.6-2249jurkat<10%-2.2243439.8 *
shMAL-1374THP169-1.868022-11
shMAL-1504THP165-1.548001-3.8
shMYD88-1830THP1733.0653132.7
shMYD88-2207THP153-1.315212-4
shTLR2-1625THP159-3.8590343.13
shTLR2-2271THP1571.84411014.7
shTLR4-2377THP1790.61401-2-6.6
shTLR4-1923THP1<10%-2.586143-0.1
shTLR4-806THP1<10%1.5242-2-2-0.7
shTRAF6-936THP151-0.1892340
shTRAF6-1326THP162-2.83523313.1
shTRAF6-1563THP1530.2630307.38
shTRAM-290THP1<10%1.188012-8.9
shTRAM-482THP1<10%-1.929212-1.4
shTRIF-1786THP1<10%-0.9981001.03