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Published online Jan 24, 2006. doi:  10.1186/1472-6750-6-7

Table 2

Evaluation of sequencing results of three DNA hairpin constructs. Average ratio of peak height after to before the hairpin region was determined as a measure of how well the sequence read through the hairpin structure. The greater the peak height ratio, the greater the ability to sequence through the hairpin. A value of 1 indicates no loss in peak height, and a value of zero indicates a complete stop in sequence after the hairpin region. All values are the averages of at least triplicate sequencing reactions.

DNA Plasmid
pHSPG-shTLR4pHSPG-shmutTLR4pHSPG-shmCNN3

ChemistryDNA relaxing agentPeak Height RatioPeak Height RatioPeak Height Ratio

BDv1.1None0.00.40.0
BDv1.15% DMSO0.00.60.2
BDv1.10.83 M Betaine0.30.90.7
BDv1.11 × PCRx Enhancer0.10.70.3
BDv1.10.83 M Betaine & 1 × PCRx Enhancer0.60.90.5
20:1 BD:dGTPNone0.40.60.5
20:1 BD:dGTP5% DMSO0.60.70.6
20:1 BD:dGTP0.83 M Betaine0.71.00.8
20:1 BD:dGTP1 × PCRx Enhancer0.60.80.7
20:1 BD:dGTP0.83 M Betaine & 1 × PCRx Enhancer0.80.90.8
10:1 BD:dGTPNone0.50.60.6
10:1 BD:dGTP5% DMSO0.70.80.7
10:1 BD:dGTP0.83 M Betaine0.80.90.9
10:1 BD:dGTP1 × PCRx Enhancer0.70.80.8
10:1 BD:dGTP0.83 M Betaine & 1 × PCRx Enhancer0.91.00.9
10:1 BD:dGTP1 × ThermoFidelase I0.10.20.2
5:1 BD:dGTPNone0.60.70.7
3:1 BD:dGTPNone0.60.60.6
dGTP onlyNone0.70.70.8