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Proc Natl Acad Sci U S A. Mar 28, 2006; 103(13): 5137–5142.
Published online Mar 21, 2006. doi:  10.1073/pnas.0601082103
PMCID: PMC1405910

Disruption of axo–glial junctions causes cytoskeletal disorganization and degeneration of Purkinje neuron axons


Axo–glial junctions (AGJs) play a critical role in the organization and maintenance of molecular domains in myelinated axons. Neurexin IV/Caspr1/paranodin (NCP1) is an important player in the formation of AGJs because it recruits a paranodal complex implicated in the tethering of glial proteins to the axonal membrane and cytoskeleton. Mice deficient in either the axonal protein NCP1 or the glial ceramide galactosyltransferase (CGT) display disruptions in AGJs and severe ataxia. In this article, we correlate these two phenotypes and show that both NCP1 and CGT mutants develop large swellings accompanied by cytoskeletal disorganization and degeneration in the axons of cerebellar Purkinje neurons. We also show that αII spectrin is part of the paranodal complex and that, although not properly targeted, this complex is still formed in CGT mutants. Together, these findings establish a physiologically relevant link between AGJs and axonal cytoskeleton and raise the possibility that some neurodegenerative disorders arise from disruption of the AGJs.

Keywords: myelin, paranodes, cerebellum, ataxia

The anatomical organization of myelinated fibers into distinct domains is the basis for the saltatory mode of action potential propagation. In the axons, these molecular domains (internode, juxtaparanode, paranode, and node of Ranvier) form as a result of specific polarization driven by signaling between the myelinating glial cells and neurons that has yet to be fully understood. In the paranodal region, closely apposed axon-glial membranes form specialized cell junctions, which resemble the ladder-like invertebrate septate junctions, and are referred to as paranodal septate junctions or paranodal axo–glial junctions (AGJs) (14).

Three major proteins have been shown to localize to the paranodal AGJs: NCP1 (also known as Caspr1 or paranodin) and contactin (CNTN) on the axonal side and neurofascin (NF155), the 155-kDa isoform on the glial side (59). Although NF155 is the only known glial protein at the paranodal membrane, a number of nonparanodal glial proteins are required for proper formation, maintenance, and distribution of AGJs, as in the case of ceramide galactosyltransferase (CGT), proteolipid protein, myelin-basic protein, myelin-associated glycoprotein, 2′,3′-cyclic nucleotide 3′-phosphodiesterase, and the transcription factor Nkx6–2 (1017).

Genetic ablation of NCP1 and CNTN in mice results in the loss of AGJs and a failure to segregate Na+ and K+ channels at the nodes and juxtaparanodes, respectively (5, 6). Similar phenotypes were observed at the paranodes in CGT mutants (18, 19). CGT encodes an enzyme that is needed for the biosynthesis of two important myelin lipids, galactocerebroside and sulfatide (10, 1821). Using subcellular fractionation of NF155 in detergents, Rasband and coworkers (22) proposed a model in which myelin lipids assemble in stable lipid rafts to stabilize the clustering of NF155 at the glial side of AGJs. This model is consistent with the phenotype of CGT mutants in which defective biosynthesis of components of the lipid rafts would lead to disruption of AGJs as raft microdomains may not be properly formed to allow clustering of NF155 at the paranodes. Recent studies have demonstrated that ablation of both glial (NF155) and neuronal (NF186) isoforms of neurofascin leads to disruption of both nodal and paranodal domains in myelinated fibers further strengthening the role of NF155 in AGJ formation (23).

Biochemical studies established that NCP1 associates with CNTN and that CNTN is essential for the delivery of NCP1 to paranodes in which both proteins form a high-molecular-weight complex (2426). NCP1 is the only identified AGJ axonal protein that contains a cytoplasmic domain. Evidence gathered from biochemical and transgenic mice studies showed that the cytoplasmic domain of NCP1 binds to the actin-spectrin binding protein 4.1B (27, 28). There is growing realization that NCP1 mediates AGJ interactions with the axonal cytoskeleton and may participate in axon–glial signaling; however, physiological evidence to support these assumptions remains to be established.

NCP1 and CGT mutants display severe ataxia and motor coordination defects, features commonly associated with cerebellar dysfunction. In this article, we investigated the cerebellar structure of NCP1 and CGT mutants and establish a correlation between disruption of AGJs and axonal degeneration in the cerebellum. This article points to a physiological significance of AGJs by demonstrating that axonal swellings develop when these junctions are disrupted in the Purkinje neurons.


Behavioral Studies Suggest Cerebellar Deficits in NCP1 Mutants.

Our previous studies reported that NCP1 mutant mice display a severe neurological phenotype (5). Starting at postnatal day 11 (P11), these mice display hypomotility, movement-associated tremor, severely impaired control and coordination of movement, and a wide-based gait suggestive of cerebellar defects (29, 30). To better investigate these phenotypes, we performed behavioral studies with NCP1 mutant mice. NCP1 mutant mice have an ataxic gait with a shuffling footprint pattern, a widened stance, and a lack of fore–hind foot correspondence (see Fig. 6, which is published as supporting information on the PNAS web site). We used an accelerating rotarod to assess the ability of the surviving 3-month-old NCP1 mice to coordinate movement. WT littermate control mice demonstrated normal motor coordination and balance and exhibited significant improvement in performance over successive training trials. In contrast, the NCP1 mice were able to remain on the accelerating rod for only a few seconds, and their performance did not improve across successive training trials. These data suggest impairment in cerebellar function.

Generation and Analysis of NCP1-lacZ Knockin Mice.

To precisely determine which population of cells expresses NCP1, we generated NCP1-lacZ knockin mice in which transcription of lacZ mRNA occurs in a pattern similar to that of NCP1 (Fig. 1A). Generation of heterozygous and homozygous mice was confirmed by genomic PCR analysis (Fig. 1Ba). Mice homozygous for the NCP1-lacZ allele are born at the expected Mendelian frequency from heterozygous intercrosses. These mice display an identical phenotype as that of the NCP1 mutant mice generated previously (5). Northern blot analysis by using a cDNA probe that is downstream of the insertion site confirmed that the NCP1-lacZ mutation is a null mutation because no NCP1 transcript was detected in the homozygous NCP1-lacZ mice compared with the WT (Fig. 1Bb). Western blot analysis confirmed the absence of NCP1 protein in whole-brain lysates of homozygous mutants (Fig. 1Bc).

Fig. 1.
Generation and analysis of NCP1-lacZ knockin mutants. (A) Restriction map of NCP1 locus containing the entire gene and a lacZ-neo targeting vector. (Ba) Genotyping by PCR used primers (1 + 2) amplifying a 467-bp fragment for the WT and primers (3 + 4) ...

To assess the expression pattern of NCP1-lacZ, we detected in situ activity of β-galactosidase (β-gal) in NCP1-lacZ mice at P3, P10, and P20 (Fig. 1C). β-gal activity was present in the Purkinje neurons and in the granular layer at all stages examined (Fig. 1 C and D). The schematic in Fig. 1D represents the time points analyzed according to the stage of Purkinje dendrite arborization in the molecular layer, migration of granule neurons from the external germinal layer to the granular layer, and extension of parallel fibers in the molecular layer. Note that we did not detect high β-gal activity in the molecular layer of P20 (Fig. 1D), which supports the assertion that the immunolocalization of NCP1 in the molecular layer at around P10 reflects the axonal compartmentalization of NCP1 in the parallel fibers. Although NCP1 localization in parallel fibers might be the major contributor for NCP1 staining in the molecular layer, we cannot rule out the possibility that NCP1 localizes to dendrites of Purkinje cells or that NCP1 is expressed at lower levels in interneurons in the molecular layer. Additional details about the developmental expression of NCP1 are presented in Fig. 7, which is published as supporting information on the PNAS web site. The gross morphology of the cerebellum was not affected by NCP1 ablation, and neither was the timeline for landmarks in cerebellar development (see Fig. 8, which is published as supporting information on the PNAS web site).

Mutants with Disrupted AGJs Develop Axonal Swellings in Purkinje Neurons.

NCP1 was localized at the paranodes in the WT cerebellar white matter (Fig. 2A), was absent in NCP1 mice (Fig. 2B), and showed a diffuse distribution at the paranodal region in the CGT mice (Fig. 2C), consistent with refs. 5, 18, and 31. Interestingly, calbindin staining of the Purkinje neurons demonstrated large numbers of focal axonal swellings in both NCP1 and CGT mutants from P10 onwards (Fig. 2 DI). These swellings were observed in the myelinated regions distal to the axon initial segment (see the arrowheads in Fig. 2E and F), and they were not observed in the Purkinje axons of WT littermates (Fig. 2D). At a higher resolution, Purkinje axons in NCP1 (Fig. 2H) and CGT (Fig. 2I) mutant mice frequently displayed several consecutive swellings on the axon resembling beads on a string (arrowheads; compare with WT in Fig. 2G), some of them reaching 10–15 times the thickness of a normal myelinated axon. These data suggest that the loss of AGJs in both NCP1 and CGT mutants results in the development of swellings in the Purkinje neuron axons.

Fig. 2.
Purkinje neuron axonal swellings in NCP1 and CGT mutants. WT (A), NCP1 (B), and CGT (C) mutant cerebellar white matter coimmunostained against NCP1 at paranodes (green; arrowheads) and calbindin (blue; axons). Red arrowheads point to paranodal regions ...

Axonal Swellings Display Organelle Accumulation and Cytoskeletal Disorganization and Were Associated with Purkinje Axon Degeneration.

Disruptions in axonal transport are known to cause swellings containing aggregates of cellular organelles and proteins, such as mitochondria and neurofilaments that are normally transported along the axons. To determine whether mitochondria were accumulating in these swellings, we immunostained cerebella from WT (Fig. 2J), NCP1 (Fig. 2K), and CGT (Fig. 2L) mutants with anti-cytochrome c antibody, a marker for mitochondria. We found that cytochrome c immunoreactivity was enhanced in the swellings in NCP1 and CGT axons when compared with WT axons (Fig. 2 JL; arrowheads). Similar axonal swellings have also been reported in PLP mutants in which the accumulation of mitochondria was attributed to impairment in fast axonal transport and associated with the accumulation of phosphorylated neurofilaments (32). We therefore determined the distribution of phosphorylated neurofilaments in NCP1 and CGT mutant Purkinje axons. As shown in Fig. 2 MO (arrowheads), the axonal swellings in NCP1 and CGT mutants also accumulated phosphorylated neurofilaments, suggesting that axonal transport was impaired in NCP1 and CGT mutant axons. Interestingly, costaining by using markers for Purkinje axonal swellings (anti-calbindin) and juxtaparanodes (anti-Kv1.1) indicated that the juxtaparanodes in NCP1 mutants flank small, and probably nascent, axonal swellings close to the white matter (Fig. 2 QR), which present a much more irregular diameter when compared with the WT (Fig. 2P). We did not detect clustering of Kv1.1 flanking large swellings, an indication that large swelling sizes were accompanied by diffusion of the juxtaparanodal K+ channels.

To investigate the ultrastructure of these swellings, we performed transmission electron microscopy (TEM) of longitudinal sections of the Purkinje axons. Consistent with previous work, our TEM data show that axons from WT mice displayed transverse septa, which link the paranodal myelin loops with axolemma (Fig. 3A; arrowheads). In both the NCP1 and CGT mutant mice, paranodal transverse septa were absent (Fig. 3 B and C; arrowheads) (5, 31). At the swellings, the NCP1 and CGT mutant axons displayed severely disorganized and misoriented microtubules (arrows) and neurofilaments (arrowheads) (Fig. 3 E and G) in contrast to those of the WT axons in which these cytoskeletal elements were arranged in parallel arrays (Fig. 3D). This disorganization was particularly obvious at higher magnifications (Fig. 3 I and J) when compared with the WT (Fig. 3H). Consistent with immunofluorescence (Fig. 2), we observed that mitochondria accumulated proximal to the paranodal region (Fig. 3F). Taken together, the ultrastructural analyses indicate that AGJ disruption in NCP1 and CGT mutants resulted in disorganized cytoskeleton and abnormal organelle accumulation specifically in the proximity of the paranodal regions.

Fig. 3.
Cytoskeletal abnormalities in Purkinje neuron axons in NCP1 and CGT mutants. (A) WT myelinated axons show paranodal AGJs with clearly visible transverse septa (arrowheads). (B) In NCP1 mutants, paranodal AGJ formation is abnormal and transverse septa ...

To better visualize the ultrastructure of the axonal swellings in an effort to determine how they affect axonal diameter, we performed TEM analysis of the Purkinje axon swellings (Fig. 4). In NCP1 (Fig. 4B) and CGT (Fig. 4C) mutants, we detected large axonal accumulations of organelles resulting in dramatically enlarged axon caliber compared with WT (Fig. 4A). Consistent with our immunofluorescence data (Fig. 2 P and Q), we found that the axonal swellings frequently form within or close to the paranodal region (see arrows in Fig. 4 AC). In addition, the most frequent components of the swellings were mitochondria (arrowheads in Fig. 4 B and C) and smooth endoplasmic reticulum (SER). The accumulation of SER was so extreme that it forms a lattice filling most of the mutant axon (Fig. 4 DF; arrowheads). As postnatal development continued, axonal degeneration became more prominent, as evidenced by vacuolation and fragmentation of the axonal cytoskeleton in both NCP1 (Fig. 4 G and H; arrowheads) and CGT (Fig. 4I; arrowheads) mutants. Ultrastructural analysis of the molecular layer in NCP1 and CGT mutants did not reveal any significant differences in synaptic density compared with WT (see Fig. 9, which is published as supporting information on the PNAS web site). Taken together, the immunofluorescence and TEM analyses show that disruption of AGJs lead to paranodal cytoskeletal disorganization and eventual degeneration of the Purkinje axons.

Fig. 4.
Organelle accumulation and axonal degeneration in NCP1 and CGT mutants. (A) Section of a WT axon through the paranodal region (arrows) showing normal axonal diameter and cytoskeletal organization. (B) An NCP1 mutant axon shows a large swelling at the ...

αII Spectrin Is Part of the Paranodal Complex Recruited by NCP1.

In the paranodal region, the axolemma and the lateral loops of the myelin sheath maintain their closest apposition to establish the paranodal AGJs (14). Previous studies revealed that NCP1 recruits a complex containing axonal proteins resident at both the AGJs and cytoskeleton (2, 33, 34). The cytoskeletal disorganization observed in the NCP1 and CGT mutants suggest that the molecular link between AGJs and the axonal cytoskeleton is important for local arrangement of the cytoskeletal network in Purkinje axons. Other proteins identified as part of the paranodal complex include CNTN and a brain-specific isoform of actin/spectrin-binding protein, band 4.1B (27). We therefore carried out immunoprecipitation experiments using anti-NCP1 antibodies on membrane-enriched cerebellar lysates from WT and NCP1 and CGT mutants. Together with proteins reported to be part of the paranodal complex, we identified two additional cytoskeletal proteins in our immunoprecipitates: actin and the axon-specific neuronal spectrin αII spectrin (240 kDa) (Fig. 5A; see the +/+ column) (35, 36). Although actin is a logical candidate to be part of the complex, our results provide biochemical evidence for this assumption and strongly support the idea that the AGJs are linked to the axonal cytoskeleton. The presence of αII spectrin in NCP1 immunoprecipitates was detected by a fodrin antibody and further confirmed by αII spectrin-specific antibodies (data not shown). None of these proteins were immunoprecipitated from NCP1 mutant lysates (Fig. 5A; NCP1−/−) but were present in the CGT mutant immunoprecipitates (Fig. 5A; CGT−/−). Taken together, these data suggest that in the absence of AGJs in CGT mutants NCP1 is still able to biochemically recruit the identified components of the paranodal complex.

Fig. 5.
AGJs at the paranodes are linked to axonal cytoskeleton. (A) NCP1-associated protein complex immunoprecipitated with anti-NCP1 antibodies from WT (+/+), NCP1, and CGT mutant cerebellar lysates (Lys). (B) Immunostaining of WT Purkinje axons by using NCP1, ...

Recruitment of a Biochemical Paranodal Complex Is Not Sufficient for AGJ Formation.

To establish whether these proteins are present or enriched at the paranodes, we examined the subcellular localization of these proteins by immunofluorescence. As shown in Fig. 5B, NCP1 (arrowheads) and CNTN localized to the paranodes, whereas 4.1B localized to both paranodes and juxtaparanodes (Fig. 5B left column). The axonal αII spectrin was enriched at the paranodes in agreement with our biochemical observations. Immunostaining by using anti-actin antibodies, which is expressed both by glia and axons, showed a modest enrichment at the paranodes in the WT axons (Fig. 5B left column). The paranodal localization of these proteins showed diffuse distribution in NCP1 and CGT mutant axons and were not enriched at the paranodes except for NCP1, which is absent in NCP1 mutants (Fig. 5B middle and right columns, respectively). Taken together, the biochemical experiments indicated that NCP1 links paranodal AGJs to axonal cytoskeleton through 4.1B and αII spectrin and that loss (NCP1−/−) or mislocalization (CGT−/−) of NCP1 resulted in the disruption of AGJs and destabilization of the axonal cytoskeleton around the paranodal region. To demonstrate that NCP1 colocalizes with fodrin/αII spectrin and actin at the paranodes, we performed double immunostaining. As shown in Fig. 5 C and E (arrowheads), NCP1 and fodrin/αII spectrin colocalized at the paranodes, indicating that fodrin/αII spectrin is enriched at the paranodal junctions. Similarly, immunostaining against NCP1 and actin showed some regions of colocalization in the paranodes (Fig. 5D; arrowheads), with expression of actin in the axons and the glial cells. Taken together, the biochemical and immunofluorescence data indicated that NCP1/4.1B/actin/αII spectrin form a biochemical complex, which localized to the paranodes, and that loss of NCP1 or mistargeting of the NCP1/4.1B/actin/αII spectrin complex (CGT mutants) resulted in a similar phenotype. We propose that disruption of the AGJs causes disorganization of the paranodal axonal cytoskeleton, which ultimately leads to development of axonal swellings and eventual degeneration of Purkinje neuron axons.


In the present study, we analyzed two different mouse mutants, NCP1 and CGT, which both display severe ataxia and motor coordination defects. We show that disruption of paranodal AGJs leads to cytoskeletal disorganization and degeneration of Purkinje neuron axons in the cerebellum. We also provide additional molecular evidence that AGJs are linked to the axonal cytoskeleton. Our data show that AGJs are essential not only for the organization of molecular domains in the myelinated axons (5) but also for the organization of the nodal/paranodal axonal cytoskeleton. Our studies thus establish an unappreciated role of AGJs in the maintenance of the cytoskeletal organization in myelinated axons.

Two Faces of AGJs: Glial and Neuronal.

NCP1 and CGT mutants display defective AGJs caused by very different mutations. CGT mice present genetic defects in glial cells, whereas the genetic defect in the NCP1 mutants is in the neurons. NCP1 is a key component of AGJs on the axonal side, and the disruption of AGJs in NCP1 mutants is due to the absence of this protein in the axons. In contrast to NCP1−/−, the CGT mutants express normal levels of NCP1, but the localization at the AGJs is disrupted. CGT is required for the segregation of lipid microdomains for the clustering of NF155, the glial-binding partner of the axonal paranodal complex (8, 22). Most importantly, one would not expect CGT mutation to affect NCP1 function that is not related to AGJ formation because the absence of CGT is hypothesized to affect the clustering of the NF155-based glial complex, which is the binding partner for the NCP1/CNTN-based axonal complex at AGJs.

CNTN interacts in cis with NCP1 at the AGJs and is required for cell-surface expression of NCP1 (6, 25). CNTN mutants have striking defects in cerebellar development, including axonal swellings in Purkinje neurons. CNTN is expressed by several types of cerebellar neurons and has been proposed to play a role in mediating neurite outgrowth and neuronal interactions (37, 38). However, CNTN mutant mice exhibit more widespread defects that seem specific to CNTN mutants because we did not observe any parallel fiber orientation defects in NCP1 and CGT mutants (38 and data not shown), suggesting that CNTN has additional functions which are independent of NCP1 and paranodal AGJs.

Axonal Swellings, Cytoskeletal Disorganization, and Axonal Degeneration.

Axonal swellings are induced by neurotoxic chemicals (39), genetic ablation or expression of mutated genes in transgenic mice (32, 40), and neurodegenerative diseases such as amyotrophic lateral sclerosis, Charcot–Marie–Tooth disease, Wallerian degeneration, Alzheimer's disease and cerebrospinal ataxia (4144). In all these situations, the development of swellings has been proposed to be a late sign of impairments in axonal transport. In Purkinje cells, axonal accumulation of SER has been proposed to correspond to a terminal stage of axonal degeneration (30). We found that NCP1 and CGT mice develop nodal/paranodal swellings in Purkinje axons that contain aggregates of mitochondria, SER, and neurofilaments. We also detected a strong enrichment of phosphorylated neurofilaments in the axonal swellings in NCP1 and CGT mutants. Besides its significance as an indicator of disrupted axonal transport, the phosphorylation of neurofilaments also modulates the interfilament spacing, and it is thought to be part of the signaling for myelin-dependent expansion of axonal caliber in the internodes (45). Although interactions between axons and glia are likely to regulate the axonal caliber, the mechanisms responsible for this regulation are not well understood (4, 46). Our findings raise the interesting possibility that AGJs might participate in axon–glial signaling, at least in the Purkinje neurons, to trigger stabilization and/or reorganization of axonal cytoskeleton. This idea is strengthened by our TEM data showing that Purkinje axons in NCP1 and CGT mutants display disorganized arrays of microtubules and neurofilaments.

It has been shown that NCP1 and CGT mice also have disrupted AGJs in the peripheral nerves (5, 19). We have not observed axonal swellings in the sciatic nerves of NCP1 and CGT mutants at the age that we analyzed the animals (P25). Nevertheless, Purkinje neurons present incredibly high metabolic activity and may take shorter time to develop axonal swellings before mutants die than peripheral nerves. However, Purkinje somal loss was not evident at P25 (see Fig. 10, which is published as supporting information on the PNAS web site). Our data do not allow us to rule out the possibility of inherent differences in the cytoskeletal organization and mechanisms of axonal transport between the Purkinje and other neurons. It is also possible that peripheral nerves have an inbuilt compensatory mechanism to prevent the formation of the axonal swellings. A better understanding of the differences in the molecular architecture of the peripheral and central paranodal/nodal regions may be required to resolve some of these differences.

αII Spectrin: A Player in the Complex Recruited by NCP1 to Link AGJs to Axonal Cytoskeleton.

Our biochemical studies showed that NCP1 forms a complex with actin and two actin-binding proteins: 4.1B and αII spectrin, which are members of protein families that associate with actin and transmembrane proteins to modulate the shape of cytoskeleton-linked membranes. Brain-specific αII spectrin has been reported to localize to myelinated axons in the cerebellum (36). Our data show that NCP1 recruited a protein complex on the axonal side of the paranodes, through which it links the AGJs to the axonal cytoskeleton. In CGT mutants, NCP1 retains the ability to recruit this complex despite its failure to organize the AGJs in these animals. The immunostaining data from CGT mutants raise an interesting possibility that the mere presence of NCP1 in the axons is not sufficient to establish or stabilize paranodal AGJs; rather, it is the proper localization of NCP1 at the paranodes that is critical for the normal organization of the axonal cytoskeleton at the paranodes.

AGJs and Relevance to Human Diseases.

Human diseases such as multiple sclerosis (MS) are known to cause demyelination and axonal degeneration, but the mechanisms that lead to axonal degeneration remain largely unclear (47). Recent studies show that NCP1 is diffused along the axons in MS lesions, suggesting that the aberrant location of NCP1 is an early sign of impending myelin loss in MS (48). In addition, NCP1-positive regions on some myelinated axons presented increased axonal caliber near the lesion edges (48). Although MS is a human disease, we show that two different mouse models of AGJ disruption presented axonal swellings close to the putative paranodal region and that the paranodal complex and/or its components were diffused in these axons. Our studies raise an interesting possibility that the pathogenesis resulting from various myelin disorders may actually be linked to compromised AGJs, leading to cytoskeletal disorganization, axonal neurodegeneration, and severe motor deficits.

Materials and Methods

Detailed experimental procedures are provided in Supporting Materials and Methods, which is published as supporting information on the PNAS web site.


The immunofluorescence methods have been described in ref. 5. The antibodies used were anti-NCP1 (5), anti-calbindin (Sigma), anti-GFAP (Chemicon), anti-cytochrome c (M. Deshmukh, University of North Carolina, Chapel Hill), anti-phospho-neurofilaments (Sternberger Monoclonals, Lutherville, MD), anti Kv1.1 channels (Sigma), anti-CNTN (J. Salzer, New York University, New York; and S. Harroch, the Pasteur Institute, Paris), anti-4.1B (P. Gascard, the Lawrence Berkeley National Laboratory, Berkeley, CA), anti-fodrin (R. Cheney, University of North Carolina), anti-αII spectrin (S. Goodman, University of Texas, Dallas), and anti-actin (Chemicon).

NCP1-lacZ Knockin Mice and β-Gal Activity.

129Sv/Ev mouse genomic phage clones were isolated to establish a contiguous map of the NCP1 locus. The construction of targeting vectors and analysis of the targeted embryonic stem cells to obtain NCP1-lacZ mice was carried out as described in ref. 5. For detection of β-gal, 30-μm sections were rinsed four to five times in PBS and stained at 37°C in dark for variable times with β-gal-staining solution [100 mM K3Fe(CN)6/5 mM K4Fe(CN)6/40 mg/ml 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside/2 mM MgCl2 in PBS]. The color development was terminated by a quick wash in PBS before mounting.


Ultrastructural analysis of WT, NCP1, and CGT mutants was carried out as described in ref. 31.

Supplementary Material

Supporting Information:


We thank R. Cheney, M. Deshmukh, P. Gascard, S. Goodman, S. Harroch, and J. Salzer for reagents; S. Honigbaum and K. McNaughton for microscopy assistance; J. Anderson, H. Bellen, R. Cheney, A. Fanning, A. Gow, B. Philpot, K. Suzuki, and the Bhat laboratory members for comments and discussion. This work was supported by National Institutes of Health Grants GM63074 and CA78437 (to M.A.B.) and funds from the State of North Carolina.


axo–glial junctions
Neurexin IV/Caspr1/paranodin
ceramide galactosyltransferase
postnatal day n
transmission electron microscopy
smooth endoplasmic reticulum
multiple sclerosis


Conflict of interest statement: No conflicts declared.


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