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Appl Environ Microbiol. Apr 1997; 63(4): 1357–1361.
PMCID: PMC1389549

Role of 2,4-Diacetylphloroglucinol in the Interactions of the Biocontrol Pseudomonad Strain F113 with the Potato Cyst Nematode Globodera rostochiensis


The potato cyst nematode Globodera rostochiensis is an important pest of potato (Solanum tuberosum). Pseudomonas fluorescens F113, which produces 2,4-diacetylphloroglucinol (DAPG), was investigated as a potential biocontrol agent against G. rostochiensis. Exposure of nematode cysts to the pseudomonad, under in vitro conditions or in soil microcosms, almost doubled the ability of the eggs to hatch. The percentage of mobile juveniles was reduced threefold following their incubation in the presence of the pseudomonad, both in vitro and in soil. Results obtained with a transposon-induced DAPG-negative biosynthetic mutant of F113 and its complemented derivative with restored DAPG synthesis showed that the ability of strain F113 to produce DAPG was responsible for the increase in hatch ability and the reduction in juvenile mobility. Similar effects on egg hatch ability and juvenile mobility of G. rostochiensis were obtained in vitro by incubating nematode cysts and juveniles, respectively, in the presence of synthetic DAPG. DAPG-producing P. fluorescens F113 is proposed as a potential biocontrol inoculant for the protection of potato crops against the potato cyst nematode.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Carroll H, Moenne-Loccoz Y, Dowling DN, O'gara F. Mutational Disruption of the Biosynthesis Genes Coding for the Antifungal Metabolite 2,4-Diacetylphloroglucinol Does Not Influence the Ecological Fitness of Pseudomonas fluorescens F113 in the Rhizosphere of Sugarbeets. Appl Environ Microbiol. 1995 Aug;61(8):3002–3007. [PMC free article] [PubMed]
  • Clarke AJ, Shepherd AM. Picrolonic acid as a hatching agent for the potato cyst nematode, Heterodera rostochiensis Woll. Nature. 1966 Jul 30;211(5048):546–546. [PubMed]
  • Fenton AM, Stephens PM, Crowley J, O'Callaghan M, O'Gara F. Exploitation of gene(s) involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain. Appl Environ Microbiol. 1992 Dec;58(12):3873–3878. [PMC free article] [PubMed]
  • Meadows J, Gill SS, Bone LW. Factors influencing lethality of Bacillus thuringiensis kurstaki toxin for eggs and larvae of Trichostrongylus colubriformis (Nematoda). J Parasitol. 1989 Apr;75(2):191–194. [PubMed]
  • Shanahan P, O'sullivan DJ, Simpson P, Glennon JD, O'gara F. Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production. Appl Environ Microbiol. 1992 Jan;58(1):353–358. [PMC free article] [PubMed]

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