• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Jan 1997; 63(1): 246–253.
PMCID: PMC1389104

Genomic Variability of the Xanthomonas Pathovar mangiferaeindicae, Agent of Mango Bacterial Black Spot


The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an hrp cluster probe from Xanthomonas oryzae pv. oryzae separated 11 of the strains from all of the other strains, which suggested that these 11 strains may not be Xanthomonas pv. mangiferaeindicae strains. Hybridization with an avirulence gene from X. oryzae pv. oryzae and a repetitive DNA fragment from Xanthomonas pv. mangiferaeindicae separated the remaining 127 strains into four groups that were consistent with both geographic and host origins. The group with the greatest diversity consisted of strains from Southeast Asia, where mango originated. Other groups and subgroups contained strains that were either from widely separated countries, which suggested that wide dissemination from a single site occurred, or from localized areas, which suggested that evolution of separate lineages of strains occurred. One group of strains contained only strains isolated from pepper trees in Reunion, indicating that pepper tree may not be an alternate host for Xanthomonas pv. mangiferaeindicae strains.

Full Text

The Full Text of this article is available as a PDF (387K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Adhikari TB, Cruz C, Zhang Q, Nelson RJ, Skinner DZ, Mew TW, Leach JE. Genetic Diversity of Xanthomonas oryzae pv. oryzae in Asia. Appl Environ Microbiol. 1995 Mar;61(3):966–971. [PMC free article] [PubMed]
  • Berthier Y, Thierry D, Lemattre M, Guesdon JL. Isolation of an insertion sequence (IS1051) from Xanthomonas campestris pv. dieffenbachiae with potential use for strain identification and characterization. Appl Environ Microbiol. 1994 Jan;60(1):377–384. [PMC free article] [PubMed]
  • Hopkins CM, White FF, Choi SH, Guo A, Leach JE. Identification of a family of avirulence genes from Xanthomonas oryzae pv. oryzae. Mol Plant Microbe Interact. 1992 Nov-Dec;5(6):451–459. [PubMed]
  • Leach JE, Rhoads ML, Vera Cruz CM, White FF, Mew TW, Leung H. Assessment of genetic diversity and population structure of Xanthomonas oryzae pv. oryzae with a repetitive DNA element. Appl Environ Microbiol. 1992 Jul;58(7):2188–2195. [PMC free article] [PubMed]
  • Legard DE, Aquadro CF, Hunter JE. DNA sequence variation and phylogenetic relationships among strains of Pseudomonas syringae pv. syringae inferred from restriction site maps and restriction fragment length polymorphism. Appl Environ Microbiol. 1993 Dec;59(12):4180–4188. [PMC free article] [PubMed]
  • Maas R. An improved colony hybridization method with significantly increased sensitivity for detection of single genes. Plasmid. 1983 Nov;10(3):296–298. [PubMed]
  • Nelson RJ, Baraoidan MR, Cruz CM, Yap IV, Leach JE, Mew TW, Leung H. Relationship between Phylogeny and Pathotype for the Bacterial Blight Pathogen of Rice. Appl Environ Microbiol. 1994 Sep;60(9):3275–3283. [PMC free article] [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. [PubMed]
  • Scholz BK, Jakobek JL, Lindgren PB. Restriction fragment length polymorphism evidence for genetic homology within a pathovar of Pseudomonas syringae. Appl Environ Microbiol. 1994 Apr;60(4):1093–1100. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...