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Appl Environ Microbiol. Mar 1996; 62(3): 822–824.
PMCID: PMC1388797

DNA Extraction and PCR Methods for the Detection of Listeria monocytogenes in Cold-Smoked Salmon

Abstract

Protocols for the specific detection of Listeria monocytogenes in cold-smoked salmon were developed. PCR was used as the method of detection. Inhibitors of PCR present in the food samples were removed by ether extraction or column purification, or their effect was overcome by the use of Tween 20 as an enhancer. These protocols are many times more rapid than conventional detection methodologies and also have the potential for automation.

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Selected References

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  • Ben Embarek PK. Presence, detection and growth of Listeria monocytogenes in seafoods: a review. Int J Food Microbiol. 1994 Sep;23(1):17–34. [PubMed]
  • Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. [PMC free article] [PubMed]
  • Buchanan RL, Stahl HG, Bencivengo MM, Del Corral F. Comparison of lithium chloride-phenylethanol-moxalactam and modified Vogel Johnson agars for detection of Listeria spp. in retail-level meats, poultry, and seafood. Appl Environ Microbiol. 1989 Mar;55(3):599–603. [PMC free article] [PubMed]
  • Dillon R, Patel T, Ratnam S. Occurrence of Listeria in hot and cold smoked seafood products. Int J Food Microbiol. 1994 Apr;22(1):73–77. [PubMed]
  • Kerr KG, Lacey RW. ACP Broadsheet 129: August 1991. Isolation and identification of Listeria monocytogenes. J Clin Pathol. 1991 Aug;44(8):624–627. [PMC free article] [PubMed]
  • Leimeister-Wächter M, Haffner C, Domann E, Goebel W, Chakraborty T. Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of listeria monocytogenes. Proc Natl Acad Sci U S A. 1990 Nov;87(21):8336–8340. [PMC free article] [PubMed]
  • Murray MG, Thompson WF. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980 Oct 10;8(19):4321–4325. [PMC free article] [PubMed]
  • Rossen L, Nørskov P, Holmstrøm K, Rasmussen OF. Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Microbiol. 1992 Sep;17(1):37–45. [PubMed]
  • Starbuck MA, Hill PJ, Stewart GS. Ultra sensitive detection of Listeria monocytogenes in milk by the polymerase chain reaction (PCR). Lett Appl Microbiol. 1992 Dec;15(6):248–252. [PubMed]
  • Wernars K, Heuvelman CJ, Chakraborty T, Notermans SH. Use of the polymerase chain reaction for direct detection of Listeria monocytogenes in soft cheese. J Appl Bacteriol. 1991 Feb;70(2):121–126. [PubMed]
  • Wernars K, Heuvelman K, Notermans S, Domann E, Leimeister-Wächter M, Chakraborty T. Suitability of the prfA gene, which encodes a regulator of virulence genes in Listeria monocytogenes, in the identification of pathogenic Listeria spp. Appl Environ Microbiol. 1992 Feb;58(2):765–768. [PMC free article] [PubMed]

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