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Immunology. Nov 1995; 86(3): 343–350.
PMCID: PMC1383935

Regulation and targeting of T-cell immune responses by IgE and IgG antibodies.

Abstract

A set of chimeric antibodies with identical F(ab')2 fragments specific for the hapten 5-iodo-4-hydroxyl-3-nitrophenacetyl (NIP), but with different human Fc parts (gamma 1, gamma 2, gamma 3, gamma 4, epsilon), was used to compare the role of IgG and IgE antibodies in antigen presentation by human Epstein-Barr virus (EBV) B cells. Two or three molecules of NIP were coupled to one molecule of Der pI (Der pI-(3)NIP), a major allergen of Dermatophagoides pteronyssinus. Both monomeric IgG and performed complexes of various Der pI/IgG ratios failed to bind significantly to the Fc receptor for IgG on B cells (Fc gamma RII; CD32). Binding of IgG3 (> IgG1)-containing complexes (optimal ratio of antigen to antibody = 1:1) could be enhanced by increasing the number of haptens per Der pI molecule to nine or more. However, antigen presentation mediated by IgG and CD32 was not seen with either pulsed B cells or B cells that were allowed to capture the IgG complexes during the whole stimulation period. IgE binding to CD23 and subsequent IgE-mediated antigen presentation was seen under all conditions tested. Even monomeric immune complexes (IC) (Der pI-(3)NIP/IgE), in the absence of CD23 cross-linking, induced an immune response. As the number of natural epitopes for human antibodies on Der pI was less than five, we conclude that, in vivo, complexes consisting of Der pI/IgG will be directed to antigen-presenting cells expressing the high-affinity receptor for IgG (CD64), whereas IgE will allow antigen presentation by CD23-expressing cells, including B cells.

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Selected References

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  • Gordon J, Flores-Romo L, Cairns JA, Millsum MJ, Lane PJ, Johnson GD, MacLennan IC. CD23: a multi-functional receptor/lymphokine? Immunol Today. 1989 May;10(5):153–157. [PubMed]
  • Rousselet G, Billaud M, Busson P, Lenoir G, Tursz T. CD23 and epithelial cells. Monogr Allergy. 1991;29:186–195. [PubMed]
  • Fujiwara H, Kikutani H, Suematsu S, Naka T, Yoshida K, Yoshida K, Tanaka T, Suemura M, Matsumoto N, Kojima S, et al. The absence of IgE antibody-mediated augmentation of immune responses in CD23-deficient mice. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):6835–6839. [PMC free article] [PubMed]
  • Mudde GC, Van Reijsen FC, Boland GJ, de Gast GC, Bruijnzeel PL, Bruijnzeel-Koomen CA. Allergen presentation by epidermal Langerhans' cells from patients with atopic dermatitis is mediated by IgE. Immunology. 1990 Mar;69(3):335–341. [PMC free article] [PubMed]
  • Maurer D, Ebner C, Reininger B, Fiebiger E, Kraft D, Kinet JP, Stingl G. The high affinity IgE receptor (Fc epsilon RI) mediates IgE-dependent allergen presentation. J Immunol. 1995 Jun 15;154(12):6285–6290. [PubMed]
  • Pirron U, Schlunck T, Prinz JC, Rieber EP. IgE-dependent antigen focusing by human B lymphocytes is mediated by the low-affinity receptor for IgE. Eur J Immunol. 1990 Jul;20(7):1547–1551. [PubMed]
  • Santamaria LF, Bheekha R, van Reijsen FC, Perez Soler MT, Suter M, Bruijnzeel-Koomen CA, Mudde GC. Antigen focusing by specific monomeric immunoglobulin E bound to CD23 on Epstein-Barr virus-transformed B cells. Hum Immunol. 1993 May;37(1):23–30. [PubMed]
  • Mudde GC, Bheekha R, Bruijnzeel-Koomen CA. Consequences of IgE/CD23-mediated antigen presentation in allergy. Immunol Today. 1995 Aug;16(8):380–383. [PubMed]
  • Sallusto F, Lanzavecchia A. Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha. J Exp Med. 1994 Apr 1;179(4):1109–1118. [PMC free article] [PubMed]
  • Gosselin EJ, Wardwell K, Gosselin DR, Alter N, Fisher JL, Guyre PM. Enhanced antigen presentation using human Fc gamma receptor (monocyte/macrophage)-specific immunogens. J Immunol. 1992 Dec 1;149(11):3477–3481. [PubMed]
  • Chapman MD, Platts-Mills TA. Measurement of IgG, IgA and IgE antibodies to Dermatophagoides pteronyssinus by antigen-binding assay, using a partially purified fraction of mite extract (F4P1). Clin Exp Immunol. 1978 Oct;34(1):126–136. [PMC free article] [PubMed]
  • van Reijsen FC, Bruijnzeel-Koomen CA, Kalthoff FS, Maggi E, Romagnani S, Westland JK, Mudde GC. Skin-derived aeroallergen-specific T-cell clones of Th2 phenotype in patients with atopic dermatitis. J Allergy Clin Immunol. 1992 Aug;90(2):184–193. [PubMed]
  • Brüggemann M, Williams GT, Bindon CI, Clark MR, Walker MR, Jefferis R, Waldmann H, Neuberger MS. Comparison of the effector functions of human immunoglobulins using a matched set of chimeric antibodies. J Exp Med. 1987 Nov 1;166(5):1351–1361. [PMC free article] [PubMed]
  • Corvaïa N, Reischl IG, Kroemer E, Mudde GC. Modulation of Fc gamma receptor-mediated early events by the tyrosine phosphatase CD45 in primary human monocytes. Consequences for interleukin-6 production. Eur J Immunol. 1995 Mar;25(3):738–744. [PubMed]
  • Saha K, Case R, Wong PK. A simple method of concentrating monoclonal antibodies from culture supernatant by ultrafiltration. J Immunol Methods. 1992 Jul 6;151(1-2):307–308. [PubMed]
  • Sarmay G, Rozsnyay Z, Koncz G, Gergely J. Interaction of signaling molecules with human Fc gamma RIIb1 and the role of various Fc gamma RIIb isoforms in B-cell regulation. Immunol Lett. 1995 Jan;44(2-3):125–131. [PubMed]
  • Heymann PW, Chapman MD, Platts-Mills TA. Antigen Der f I from the dust mite Dermatophagoides farinae: structural comparison with Der p I from Dermatophagoides pteronyssinus and epitope specificity of murine IgG and human IgE antibodies. J Immunol. 1986 Nov 1;137(9):2841–2847. [PubMed]
  • Van Den Herik-Oudijk IE, Westerdaal NA, Henriquez NV, Capel PJ, Van De Winkel JG. Functional analysis of human Fc gamma RII (CD32) isoforms expressed in B lymphocytes. J Immunol. 1994 Jan 15;152(2):574–585. [PubMed]
  • Noro N, Yoshioka A, Adachi M, Yasuda K, Masuda T, Yodoi J. Monoclonal antibody (H107) inhibiting IgE binding to Fc epsilon R(+) human lymphocytes. J Immunol. 1986 Aug 15;137(4):1258–1263. [PubMed]
  • Bonnefoy JY, Guillot O, Spits H, Blanchard D, Ishizaka K, Banchereau J. The low-affinity receptor for IgE (CD23) on B lymphocytes is spatially associated with HLA-DR antigens. J Exp Med. 1988 Jan 1;167(1):57–72. [PMC free article] [PubMed]
  • Nowell J, Quaranta V. Chloroquine affects biosynthesis of Ia molecules by inhibiting dissociation of invariant (gamma) chains from alpha-beta dimers in B cells. J Exp Med. 1985 Oct 1;162(4):1371–1376. [PMC free article] [PubMed]
  • Lucisano Valim YM, Lachmann PJ. The effect of antibody isotype and antigenic epitope density on the complement-fixing activity of immune complexes: a systematic study using chimaeric anti-NIP antibodies with human Fc regions. Clin Exp Immunol. 1991 Apr;84(1):1–8. [PMC free article] [PubMed]
  • van der Heijden FL, Joost van Neerven RJ, van Katwijk M, Bos JD, Kapsenberg ML. Serum-IgE-facilitated allergen presentation in atopic disease. J Immunol. 1993 Apr 15;150(8 Pt 1):3643–3650. [PubMed]
  • van der Heijden FL, van Neerven RJ, Kapsenberg ML. Relationship between facilitated allergen presentation and the presence of allergen-specific IgE in serum of atopic patients. Clin Exp Immunol. 1995 Feb;99(2):289–293. [PMC free article] [PubMed]
  • Fridman WH. Regulation of B-cell activation and antigen presentation by Fc receptors. Curr Opin Immunol. 1993 Jun;5(3):355–360. [PubMed]
  • Daëron M, Malbec O, Latour S, Espinosa E, Pina P, Fridman WH. Regulation of tyrosine-containing activation motif-dependent cell signalling by Fc gamma RII. Immunol Lett. 1995 Jan;44(2-3):119–123. [PubMed]
  • Klausner RD, Samelson LE. T cell antigen receptor activation pathways: the tyrosine kinase connection. Cell. 1991 Mar 8;64(5):875–878. [PubMed]
  • Sármay G, Rozsnyay Z, Koncz G, Danilkovich A, Gergely J. The alternative splicing of human Fc gamma RII mRNA is regulated by activation of B cells with mIgM cross-linking, interleukin-4, or phorbolester. Eur J Immunol. 1995 Jan;25(1):262–268. [PubMed]
  • Squire CM, Studer EJ, Lees A, Finkelman FD, Conrad DH. Antigen presentation is enhanced by targeting antigen to the Fc epsilon RII by antigen-anti-Fc epsilon RII conjugates. J Immunol. 1994 May 1;152(9):4388–4396. [PubMed]
  • Kehry MR, Yamashita LC. Low-affinity IgE receptor (CD23) function on mouse B cells: role in IgE-dependent antigen focusing. Proc Natl Acad Sci U S A. 1989 Oct;86(19):7556–7560. [PMC free article] [PubMed]
  • Chen SS. Mechanisms of IgE homeostasis. Sequestration of IgE by murine type II IgE Fc receptor-bearing B cell hybridomas. J Immunol. 1991 Sep 1;147(5):1581–1586. [PubMed]
  • Bridges K, Harford J, Ashwell G, Klausner RD. Fate of receptor and ligand during endocytosis of asialoglycoproteins by isolated hepatocytes. Proc Natl Acad Sci U S A. 1982 Jan;79(2):350–354. [PMC free article] [PubMed]
  • Lanzavecchia A. Antigen-specific interaction between T and B cells. Nature. 1985 Apr 11;314(6011):537–539. [PubMed]
  • Tony HP, Phillips NE, Parker DC. Role of membrane immunoglobulin (Ig) crosslinking in membrane Ig-mediated, major histocompatibility-restricted T cell-B cell cooperation. J Exp Med. 1985 Nov 1;162(5):1695–1708. [PMC free article] [PubMed]
  • Steensgaard J, Jacobsen C, Lowe J, Ling NR, Jefferis R. Theoretical and ultracentrifugal analysis of immune complex formation between monoclonal antibodies and human IgG. Immunology. 1982 Aug;46(4):751–760. [PMC free article] [PubMed]
  • Maurer D, Fiebiger E, Reininger B, Wolff-Winiski B, Jouvin MH, Kilgus O, Kinet JP, Stingl G. Expression of functional high affinity immunoglobulin E receptors (Fc epsilon RI) on monocytes of atopic individuals. J Exp Med. 1994 Feb 1;179(2):745–750. [PMC free article] [PubMed]
  • Ernst LK, Duchemin AM, Anderson CL. Association of the high-affinity receptor for IgG (Fc gamma RI) with the gamma subunit of the IgE receptor. Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6023–6027. [PMC free article] [PubMed]
  • Mudde GC, Bheekha R, Bruijnzeel-Koomen CA. IgE-mediated antigen presentation. Allergy. 1995 Mar;50(3):193–199. [PubMed]
  • Gajewski TF, Pinnas M, Wong T, Fitch FW. Murine Th1 and Th2 clones proliferate optimally in response to distinct antigen-presenting cell populations. J Immunol. 1991 Mar 15;146(6):1750–1758. [PubMed]
  • Position paper: Immunotherapy. Allergy. 1993;48(14 Suppl):9–35. [PubMed]
  • Wyss M, Scheitlin T, Stadler BM, Wüthrich B. Immunotherapy with aluminum hydroxide adsorbed insect venom extracts (Alutard SQ): immunologic and clinical results of a prospective study over 3 years. Allergy. 1993 Feb;48(2):81–86. [PubMed]
  • Ritter C, Bättig M, Kraemer R, Stadler BM. IgE hidden in immune complexes with anti-IgE autoantibodies in children with asthma. J Allergy Clin Immunol. 1991 Nov;88(5):793–801. [PubMed]

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