• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of gutGutCurrent TOCInstructions to authors
Gut. Sep 1991; 32(9): 991–993.
PMCID: PMC1379035

Trophic action of epidermal growth factor on human duodenal mucosa cultured in vitro.

Abstract

The action of epidermal growth factor on the human duodenal mucosa has been studied by estimating the crypt cell production rate in cultured explants, using a stathmokinetic technique with crypt microdissection. The addition of epidermal growth factor (400 ng/ml) to paired explants from five patients caused an almost fivefold increase in the crypt cell production rate, showing that epidermal growth factor has a trophic action on the human duodenal mucosa in vitro.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (727K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Savage CR, Jr, Cohen S. Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. J Biol Chem. 1972 Dec 10;247(23):7609–7611. [PubMed]
  • Scheving LA, Yeh YC, Tsai TH, Scheving LE. Circadian phase-dependent stimulatory effects of epidermal growth factor on deoxyribonucleic acid synthesis in the tongue, esophagus, and stomach of the adult male mouse. Endocrinology. 1979 Dec;105(6):1475–1480. [PubMed]
  • Scheving LA, Yeh YC, Tsai TH, Scheving LE. Circadian phase-dependent stimulatory effects of epidermal growth factor on deoxyribonucleic acid synthesis in the duodenum, jejunum, ileum, caecum, colon, and rectum of the adult male mouse. Endocrinology. 1980 May;106(5):1498–1503. [PubMed]
  • Feldman EJ, Aures D, Grossman MI. Epidermal growth factor stimulates ornithine decarboxylase activity in the digestive tract of mouse. Proc Soc Exp Biol Med. 1978 Dec;159(3):400–402. [PubMed]
  • Kirkegaard P, Olsen PS, Poulsen SS, Nexø E. Epidermal growth factor inhibits cysteamine-induced duodenal ulcers. Gastroenterology. 1983 Dec;85(6):1277–1283. [PubMed]
  • Bower JM, Camble R, Gregory H, Gerring EL, Willshire IR. The inhibition of gastric acid secretion by epidermal growth factor. Experientia. 1975 Jul 15;31(7):825–826. [PubMed]
  • Malo C, Ménard D. Influence of epidermal growth factor on the development of suckling mouse intestinal mucosa. Gastroenterology. 1982 Jul;83(1 Pt 1):28–35. [PubMed]
  • Oka Y, Ghishan FK, Greene HL, Orth DN. Effect of mouse epidermal growth factor/urogastrone on the functional maturation of rat intestine. Endocrinology. 1983 Mar;112(3):940–944. [PubMed]
  • Calvert R, Beaulieu JF, Ménard D. Epidermal growth factor (EGF) accelerates the maturation of fetal mouse intestinal mucosa in utero. Experientia. 1982 Sep 15;38(9):1096–1097. [PubMed]
  • Chabot JG, Payet N, Hugon JS. Effects of epidermal growth factor (EGF) on adult mouse small intestine in vivo and in organ culture. Comp Biochem Physiol A Comp Physiol. 1983;74(2):247–252. [PubMed]
  • Ulshen MH, Lyn-Cook LE, Raasch RH. Effects of intraluminal epidermal growth factor on mucosal proliferation in the small intestine of adult rats. Gastroenterology. 1986 Nov;91(5):1134–1140. [PubMed]
  • Dembiński A, Gregory H, Konturek SJ, Polański M. Trophic action of epidermal growth factor on the pancreas and gastroduodenal mucosa in rats. J Physiol. 1982 Apr;325:35–42. [PMC free article] [PubMed]
  • Majumdar AP. Postnatal undernutrition: effect of epidermal growth factor on growth and function of the gastrointestinal tract in rats. J Pediatr Gastroenterol Nutr. 1984 Sep;3(4):618–625. [PubMed]
  • Goodlad RA, Wilson TJ, Lenton W, Gregory H, McCullagh KG, Wright NA. Intravenous but not intragastric urogastrone-EGF is trophic to the intestine of parenterally fed rats. Gut. 1987 May;28(5):573–582. [PMC free article] [PubMed]
  • Finney KJ, Ince P, Appleton DR, Sunter JP, Watson AJ. A trophic effect of epidermal growth factor (EGF) on rat colonic mucosa in organ culture. Cell Tissue Kinet. 1987 Jan;20(1):43–56. [PubMed]
  • Konturek JW, Bielanski W, Konturek SJ, Bogdal J, Oleksy J. Distribution and release of epidermal growth factor in man. Gut. 1989 Sep;30(9):1194–1200. [PMC free article] [PubMed]
  • Forgue-Lafitte ME, Laburthe M, Chamblier MC, Moody AJ, Rosselin G. Demonstration of specific receptors for EGF--urogastrone in isolated rat intestinal epithelial cells. FEBS Lett. 1980 Jun 2;114(2):243–246. [PubMed]
  • Gallo-Payet N, Hugon JS. Epidermal growth factor receptors in isolated adult mouse intestinal cells: studies in vivo and in organ culture. Endocrinology. 1985 Jan;116(1):194–201. [PubMed]
  • Conteas CN, DeMorrow JM, Majumdar AP. Effect of epidermal growth factor on growth and maturation of fetal and neonatal rat small intestine in organ culture. Experientia. 1986 Aug 15;42(8):950–952. [PubMed]
  • Ménard D, Arsenault P, Pothier P. Biologic effects of epidermal growth factor in human fetal jejunum. Gastroenterology. 1988 Mar;94(3):656–663. [PubMed]
  • Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature. 1975 Sep 25;257(5524):325–327. [PubMed]
  • Goodlad RA, Wilson TJ, Lenton W, Gregory H, McCullough KG, Wright NA. Urogastrone-epidermal growth factor is trophic to the intestinal epithelium of parenterally fed rats. Experientia. 1985 Sep 15;41(9):1161–1163. [PubMed]
  • Walker-Smith JA, Phillips AD, Walford N, Gregory H, Fitzgerald JD, MacCullagh K, Wright NA. Intravenous epidermal growth factor/urogastrone increases small-intestinal cell proliferation in congenital microvillous atrophy. Lancet. 1985 Nov 30;2(8466):1239–1240. [PubMed]
  • Wheeler EE, Challacombe DN. Influence of 5-hydroxytryptamine on crypt cell production rate of human duodenal mucosa cultured in vitro. J Clin Pathol. 1987 Jul;40(7):710–713. [PMC free article] [PubMed]

Articles from Gut are provided here courtesy of BMJ Group

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...