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Am J Hum Genet. May 1998; 62(5): 1153–1170.
PMCID: PMC1377095

mtDNA analysis of a prehistoric Oneota population: implications for the peopling of the New World.

Abstract

mtDNA was successfully extracted from 108 individuals from the Norris Farms Oneota, a prehistoric Native American population, to compare the mtDNA diversity from a pre-Columbian population with contemporary Native American and Asian mtDNA lineages and to examine hypotheses about the peopling of the New World. Haplogroup and hypervariable region I sequence data indicate that the lineages from haplogroups A, B, C, and D are the most common among Native Americans but that they were not the only lineages brought into the New World from Asia. The mtDNA evidence does not support the three-wave hypothesis of migration into the New World but rather suggests a single "wave" of people with considerable mtDNA diversity that exhibits a signature of expansion 23,000-37,000 years ago.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Anderson S, Bankier AT, Barrell BG, de Bruijn MH, Coulson AR, Drouin J, Eperon IC, Nierlich DP, Roe BA, Sanger F, et al. Sequence and organization of the human mitochondrial genome. Nature. 1981 Apr 9;290(5806):457–465. [PubMed]
  • Aris-Brosou S, Excoffier L. The impact of population expansion and mutation rate heterogeneity on DNA sequence polymorphism. Mol Biol Evol. 1996 Mar;13(3):494–504. [PubMed]
  • Bailliet G, Rothhammer F, Carnese FR, Bravi CM, Bianchi NO. Founder mitochondrial haplotypes in Amerindian populations. Am J Hum Genet. 1994 Jul;55(1):27–33. [PMC free article] [PubMed]
  • Ballinger SW, Schurr TG, Torroni A, Gan YY, Hodge JA, Hassan K, Chen KH, Wallace DC. Southeast Asian mitochondrial DNA analysis reveals genetic continuity of ancient mongoloid migrations. Genetics. 1992 Jan;130(1):139–152. [PMC free article] [PubMed]
  • Bandelt HJ, Forster P, Sykes BC, Richards MB. Mitochondrial portraits of human populations using median networks. Genetics. 1995 Oct;141(2):743–753. [PMC free article] [PubMed]
  • Batista O, Kolman CJ, Bermingham E. Mitochondrial DNA diversity in the Kuna Amerinds of Panamá. Hum Mol Genet. 1995 May;4(5):921–929. [PubMed]
  • Bendall KE, Sykes BC. Length heteroplasmy in the first hypervariable segment of the human mtDNA control region. Am J Hum Genet. 1995 Aug;57(2):248–256. [PMC free article] [PubMed]
  • Bonatto SL, Salzano FM. A single and early migration for the peopling of the Americas supported by mitochondrial DNA sequence data. Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1866–1871. [PMC free article] [PubMed]
  • Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. [PMC free article] [PubMed]
  • Cann RL, Stoneking M, Wilson AC. Mitochondrial DNA and human evolution. Nature. 1987 Jan 1;325(6099):31–36. [PubMed]
  • Chou Q, Russell M, Birch DE, Raymond J, Bloch W. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 1992 Apr 11;20(7):1717–1723. [PMC free article] [PubMed]
  • Di Rienzo A, Wilson AC. Branching pattern in the evolutionary tree for human mitochondrial DNA. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1597–1601. [PMC free article] [PubMed]
  • Easton RD, Merriwether DA, Crews DE, Ferrell RE. mtDNA variation in the Yanomami: evidence for additional New World founding lineages. Am J Hum Genet. 1996 Jul;59(1):213–225. [PMC free article] [PubMed]
  • Excoffier L, Smouse PE, Quattro JM. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics. 1992 Jun;131(2):479–491. [PMC free article] [PubMed]
  • Forster P, Harding R, Torroni A, Bandelt HJ. Origin and evolution of Native American mtDNA variation: a reappraisal. Am J Hum Genet. 1996 Oct;59(4):935–945. [PMC free article] [PubMed]
  • Lalueza Fox C. Mitochondrial DNA Haplogroups in Four Tribes from Tierra del Fuego-Patagonia: Inferences about the Peopling of the Americas. Hum Biol. 1996 Dec;68(6):853–871. [PubMed]
  • Ginther C, Corach D, Penacino GA, Rey JA, Carnese FR, Hutz MH, Anderson A, Just J, Salzano FM, King MC. Genetic variation among the Mapuche Indians from the Patagonian region of Argentina: mitochondrial DNA sequence variation and allele frequencies of several nuclear genes. EXS. 1993;67:211–219. [PubMed]
  • Hagelberg E, Clegg JB. Isolation and characterization of DNA from archaeological bone. Proc Biol Sci. 1991 Apr 22;244(1309):45–50. [PubMed]
  • Handt O, Krings M, Ward RH, Päbo S. The retrieval of ancient human DNA sequences. Am J Hum Genet. 1996 Aug;59(2):368–376. [PMC free article] [PubMed]
  • Hasegawa M, Di Rienzo A, Kocher TD, Wilson AC. Toward a more accurate time scale for the human mitochondrial DNA tree. J Mol Evol. 1993 Oct;37(4):347–354. [PubMed]
  • Hauswirth WW, Dickel CD, Rowold DJ, Hauswirth MA. Inter- and intrapopulation studies of ancient humans. Experientia. 1994 Jun 15;50(6):585–591. [PubMed]
  • Horai S, Hayasaka K. Intraspecific nucleotide sequence differences in the major noncoding region of human mitochondrial DNA. Am J Hum Genet. 1990 Apr;46(4):828–842. [PMC free article] [PubMed]
  • Horai S, Hayasaka K, Kondo R, Tsugane K, Takahata N. Recent African origin of modern humans revealed by complete sequences of hominoid mitochondrial DNAs. Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):532–536. [PMC free article] [PubMed]
  • Horai S, Kondo R, Nakagawa-Hattori Y, Hayashi S, Sonoda S, Tajima K. Peopling of the Americas, founded by four major lineages of mitochondrial DNA. Mol Biol Evol. 1993 Jan;10(1):23–47. [PubMed]
  • Horai S, Murayama K, Hayasaka K, Matsubayashi S, Hattori Y, Fucharoen G, Harihara S, Park KS, Omoto K, Pan IH. mtDNA polymorphism in East Asian Populations, with special reference to the peopling of Japan. Am J Hum Genet. 1996 Sep;59(3):579–590. [PMC free article] [PubMed]
  • Höss M, Päbo S. DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res. 1993 Aug 11;21(16):3913–3914. [PMC free article] [PubMed]
  • Kolman CJ, Bermingham E, Cooke R, Ward RH, Arias TD, Guionneau-Sinclair F. Reduced mtDNA diversity in the Ngöbé Amerinds of Panamá. Genetics. 1995 May;140(1):275–283. [PMC free article] [PubMed]
  • Kolman CJ, Sambuughin N, Bermingham E. Mitochondrial DNA analysis of Mongolian populations and implications for the origin of New World founders. Genetics. 1996 Apr;142(4):1321–1334. [PMC free article] [PubMed]
  • Krings M, Stone A, Schmitz RW, Krainitzki H, Stoneking M, Päbo S. Neandertal DNA sequences and the origin of modern humans. Cell. 1997 Jul 11;90(1):19–30. [PubMed]
  • Lahermo P, Sajantila A, Sistonen P, Lukka M, Aula P, Peltonen L, Savontaus ML. The genetic relationship between the Finns and the Finnish Saami (Lapps): analysis of nuclear DNA and mtDNA. Am J Hum Genet. 1996 Jun;58(6):1309–1322. [PMC free article] [PubMed]
  • Lum JK, Rickards O, Ching C, Cann RL. Polynesian mitochondrial DNAs reveal three deep maternal lineage clusters. Hum Biol. 1994 Aug;66(4):567–590. [PubMed]
  • Merriwether DA, Ferrell RE. The four founding lineage hypothesis for the New World: a critical reevaluation. Mol Phylogenet Evol. 1996 Feb;5(1):241–246. [PubMed]
  • Merriwether DA, Hall WW, Vahlne A, Ferrell RE. mtDNA variation indicates Mongolia may have been the source for the founding population for the New World. Am J Hum Genet. 1996 Jul;59(1):204–212. [PMC free article] [PubMed]
  • Merriwether DA, Rothhammer F, Ferrell RE. Distribution of the four founding lineage haplotypes in Native Americans suggests a single wave of migration for the New World. Am J Phys Anthropol. 1995 Dec;98(4):411–430. [PubMed]
  • Milner GR, Humpf DA, Harpending HC. Pattern matching of age-at-death distributions in paleodemographic analysis. Am J Phys Anthropol. 1989 Sep;80(1):49–58. [PubMed]
  • Morin PA, Moore JJ, Chakraborty R, Jin L, Goodall J, Woodruff DS. Kin selection, social structure, gene flow, and the evolution of chimpanzees. Science. 1994 Aug 26;265(5176):1193–1201. [PubMed]
  • Päbo S. Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification. Proc Natl Acad Sci U S A. 1989 Mar;86(6):1939–1943. [PMC free article] [PubMed]
  • Päbo S, Gifford JA, Wilson AC. Mitochondrial DNA sequences from a 7000-year old brain. Nucleic Acids Res. 1988 Oct 25;16(20):9775–9787. [PMC free article] [PubMed]
  • Parr RL, Carlyle SW, O'Rourke DH. Ancient DNA analysis of Fremont Amerindians of the Great Salt Lake Wetlands. Am J Phys Anthropol. 1996 Apr;99(4):507–518. [PubMed]
  • Redd AJ, Takezaki N, Sherry ST, McGarvey ST, Sofro AS, Stoneking M. Evolutionary history of the COII/tRNALys intergenic 9 base pair deletion in human mitochondrial DNAs from the Pacific. Mol Biol Evol. 1995 Jul;12(4):604–615. [PubMed]
  • Richards M, Côrte-Real H, Forster P, Macaulay V, Wilkinson-Herbots H, Demaine A, Papiha S, Hedges R, Bandelt HJ, Sykes B. Paleolithic and neolithic lineages in the European mitochondrial gene pool. Am J Hum Genet. 1996 Jul;59(1):185–203. [PMC free article] [PubMed]
  • Rogers AR, Harpending H. Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol. 1992 May;9(3):552–569. [PubMed]
  • Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. [PubMed]
  • Santos M, Ward RH, Barrantes R. mtDNA variation in the Chibcha Amerindian Huetar from Costa Rica. Hum Biol. 1994 Dec;66(6):963–977. [PubMed]
  • Schurr TG, Ballinger SW, Gan YY, Hodge JA, Merriwether DA, Lawrence DN, Knowler WC, Weiss KM, Wallace DC. Amerindian mitochondrial DNAs have rare Asian mutations at high frequencies, suggesting they derived from four primary maternal lineages. Am J Hum Genet. 1990 Mar;46(3):613–623. [PMC free article] [PubMed]
  • Sherry ST, Rogers AR, Harpending H, Soodyall H, Jenkins T, Stoneking M. Mismatch distributions of mtDNA reveal recent human population expansions. Hum Biol. 1994 Oct;66(5):761–775. [PubMed]
  • Shields GF, Schmiechen AM, Frazier BL, Redd A, Voevoda MI, Reed JK, Ward RH. mtDNA sequences suggest a recent evolutionary divergence for Beringian and northern North American populations. Am J Hum Genet. 1993 Sep;53(3):549–562. [PMC free article] [PubMed]
  • Slatkin M, Hudson RR. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics. 1991 Oct;129(2):555–562. [PMC free article] [PubMed]
  • Stone AC, Milner GR, Päbo S, Stoneking M. Sex determination of ancient human skeletons using DNA. Am J Phys Anthropol. 1996 Feb;99(2):231–238. [PubMed]
  • Stone AC, Stoneking M. Ancient DNA from a pre-Columbian Amerindian population. Am J Phys Anthropol. 1993 Dec;92(4):463–471. [PubMed]
  • Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989 Nov;123(3):585–595. [PMC free article] [PubMed]
  • Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993 May;10(3):512–526. [PubMed]
  • Torroni A, Chen YS, Semino O, Santachiara-Beneceretti AS, Scott CR, Lott MT, Winter M, Wallace DC. mtDNA and Y-chromosome polymorphisms in four Native American populations from southern Mexico. Am J Hum Genet. 1994 Feb;54(2):303–318. [PMC free article] [PubMed]
  • Torroni A, Miller JA, Moore LG, Zamudio S, Zhuang J, Droma T, Wallace DC. Mitochondrial DNA analysis in Tibet: implications for the origin of the Tibetan population and its adaptation to high altitude. Am J Phys Anthropol. 1994 Feb;93(2):189–199. [PubMed]
  • Torroni A, Neel JV, Barrantes R, Schurr TG, Wallace DC. Mitochondrial DNA "clock" for the Amerinds and its implications for timing their entry into North America. Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1158–1162. [PMC free article] [PubMed]
  • Torroni A, Schurr TG, Cabell MF, Brown MD, Neel JV, Larsen M, Smith DG, Vullo CM, Wallace DC. Asian affinities and continental radiation of the four founding Native American mtDNAs. Am J Hum Genet. 1993 Sep;53(3):563–590. [PMC free article] [PubMed]
  • Torroni A, Schurr TG, Yang CC, Szathmary EJ, Williams RC, Schanfield MS, Troup GA, Knowler WC, Lawrence DN, Weiss KM, et al. Native American mitochondrial DNA analysis indicates that the Amerind and the Nadene populations were founded by two independent migrations. Genetics. 1992 Jan;130(1):153–162. [PMC free article] [PubMed]
  • Torroni A, Wallace DC. MtDNA haplogroups in Native Americans. Am J Hum Genet. 1995 May;56(5):1234–1238. [PMC free article] [PubMed]
  • Turner CG., 2nd Advances in the dental search for Native American origins. Acta Anthropogenet. 1984;8(1-2):23–78. [PubMed]
  • Ubelaker DH. Patterns of demographic change in the Americas. Hum Biol. 1992 Jun;64(3):361–379. [PubMed]
  • Vigilant L, Pennington R, Harpending H, Kocher TD, Wilson AC. Mitochondrial DNA sequences in single hairs from a southern African population. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9350–9354. [PMC free article] [PubMed]
  • Wakeley J. Substitution rate variation among sites in hypervariable region 1 of human mitochondrial DNA. J Mol Evol. 1993 Dec;37(6):613–623. [PubMed]
  • Wallace DC, Garrison K, Knowler WC. Dramatic founder effects in Amerindian mitochondrial DNAs. Am J Phys Anthropol. 1985 Oct;68(2):149–155. [PubMed]
  • Wallace DC, Torroni A. American Indian prehistory as written in the mitochondrial DNA: a review. Hum Biol. 1992 Jun;64(3):403–416. [PubMed]
  • Ward RH, Frazier BL, Dew-Jager K, Päbo S. Extensive mitochondrial diversity within a single Amerindian tribe. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8720–8724. [PMC free article] [PubMed]
  • Ward RH, Redd A, Valencia D, Frazier B, Päbo S. Genetic and linguistic differentiation in the Americas. Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10663–10667. [PMC free article] [PubMed]
  • Watson E, Bauer K, Aman R, Weiss G, von Haeseler A, Päbo S. mtDNA sequence diversity in Africa. Am J Hum Genet. 1996 Aug;59(2):437–444. [PMC free article] [PubMed]
  • Wrischnik LA, Higuchi RG, Stoneking M, Erlich HA, Arnheim N, Wilson AC. Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA. Nucleic Acids Res. 1987 Jan 26;15(2):529–542. [PMC free article] [PubMed]

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