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RNA. Mar 2002; 8(3): 290–295.
PMCID: PMC1370251

Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P.


In HeLa cells, ribonuclease P (RNase P), the tRNA processing enzyme consists of an RNA subunit (H1 RNA) associated with at least nine protein subunits, Rpp14, Rpp20, Rpp21, Rpp29 (hPop4), Rpp30, Rpp38, Rpp40, hPop1, and hPop5 (18.8 kDa). We report here the cloning and immuno-biochemical analysis of Rpp25, another protein subunit of RNase P. Polyclonal rabbit antibodies raised against recombinant Rpp25 recognize their corresponding antigens in RNase P-containing fractions purified from HeLa cells, and they also precipitate active holoenzyme. Furthermore, this protein has general RNA binding properties.

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Selected References

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  • Altman S, Wesolowski D, Puranam RS. Nucleotide sequences of the RNA subunit of RNase P from several mammals. Genomics. 1993 Nov;18(2):418–422. [PubMed]
  • Bartkiewicz M, Gold H, Altman S. Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells. Genes Dev. 1989 Apr;3(4):488–499. [PubMed]
  • Burd CG, Dreyfuss G. Conserved structures and diversity of functions of RNA-binding proteins. Science. 1994 Jul 29;265(5172):615–621. [PubMed]
  • Chen JL, Pace NR. Identification of the universally conserved core of ribonuclease P RNA. RNA. 1997 Jun;3(6):557–560. [PMC free article] [PubMed]
  • Doria M, Carrara G, Calandra P, Tocchini-Valentini GP. An RNA molecule copurifies with RNase P activity from Xenopus laevis oocytes. Nucleic Acids Res. 1991 May 11;19(9):2315–2320. [PMC free article] [PubMed]
  • Eder PS, Kekuda R, Stolc V, Altman S. Characterization of two scleroderma autoimmune antigens that copurify with human ribonuclease P. Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1101–1106. [PMC free article] [PubMed]
  • Eder PS, Srinivasan A, Fishman MC, Altman S. The RNA subunit of ribonuclease P from the zebrafish, Danio rerio. J Biol Chem. 1996 Aug 30;271(35):21031–21036. [PubMed]
  • Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell. 1983 Dec;35(3 Pt 2):849–857. [PubMed]
  • Guerrier-Takada C, Lumelsky N, Altman S. Specific interactions in RNA enzyme-substrate complexes. Science. 1989 Dec 22;246(4937):1578–1584. [PubMed]
  • Jarrous N, Eder PS, Guerrier-Takada C, Hoog C, Altman S. Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. RNA. 1998 Apr;4(4):407–417. [PMC free article] [PubMed]
  • Jarrous N, Eder PS, Wesolowski D, Altman S. Rpp14 and Rpp29, two protein subunits of human ribonuclease P. RNA. 1999 Feb;5(2):153–157. [PMC free article] [PubMed]
  • Jarrous N, Reiner R, Wesolowski D, Mann H, Guerrier-Takada C, Altman S. Function and subnuclear distribution of Rpp21, a protein subunit of the human ribonucleoprotein ribonuclease P. RNA. 2001 Aug;7(8):1153–1164. [PMC free article] [PubMed]
  • Jiang T, Guerrier-Takada C, Altman S. Protein-RNA interactions in the subunits of human nuclear RNase P. RNA. 2001 Jul;7(7):937–941. [PMC free article] [PubMed]
  • Krupp G, Thurianx P, Willis I, Gamulin V, Söll D. First identification of an amber nonsense mutation in Schizosaccharomyces pombe: major differences in the efficiency of homologous versus heterologous yeast suppressor tRNA genes. Mol Gen Genet. 1985;201(1):82–87. [PubMed]
  • Lee YC, Lee BJ, Hwang DS, Kang HS. Purification and characterization of mitochondrial ribonuclease P from Aspergillus nidulans. Eur J Biochem. 1996 Jan 15;235(1-2):289–296. [PubMed]
  • Lygerou Z, Pluk H, van Venrooij WJ, Séraphin B. hPop1: an autoantigenic protein subunit shared by the human RNase P and RNase MRP ribonucleoproteins. EMBO J. 1996 Nov 1;15(21):5936–5948. [PMC free article] [PubMed]
  • Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. [PMC free article] [PubMed]
  • van Eenennaam H, Lugtenberg D, Vogelzangs JH, van Venrooij WJ, Pruijn GJ. hPop5, a protein subunit of the human RNase MRP and RNase P endoribonucleases. J Biol Chem. 2001 Aug 24;276(34):31635–31641. [PubMed]
  • van Eenennaam H, Pruijn GJ, van Venrooij WJ. hPop4: a new protein subunit of the human RNase MRP and RNase P ribonucleoprotein complexes. Nucleic Acids Res. 1999 Jun 15;27(12):2465–2472. [PMC free article] [PubMed]
  • Xiao S, Houser-Scott F, Engelke DR. Eukaryotic ribonuclease P: increased complexity to cope with the nuclear pre-tRNA pathway. J Cell Physiol. 2001 Apr;187(1):11–20. [PMC free article] [PubMed]

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