• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of rnaThe RNA SocietyeTOC AlertsSubscriptionsJournal HomeCSHL PressRNA
RNA. Sep 2000; 6(9): 1197–1211.
PMCID: PMC1369994

Sorting out the complexity of SR protein functions.

Full Text

The Full Text of this article is available as a PDF (283K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abovich N, Rosbash M. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals. Cell. 1997 May 2;89(3):403–412. [PubMed]
  • Amrein H, Gorman M, Nöthiger R. The sex-determining gene tra-2 of Drosophila encodes a putative RNA binding protein. Cell. 1988 Dec 23;55(6):1025–1035. [PubMed]
  • Amrein H, Hedley ML, Maniatis T. The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2. Cell. 1994 Feb 25;76(4):735–746. [PubMed]
  • Anderson K, Moore MJ. Bimolecular exon ligation by the human spliceosome. Science. 1997 Jun 13;276(5319):1712–1716. [PubMed]
  • Ayane M, Preuss U, Köhler G, Nielsen PJ. A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs. Nucleic Acids Res. 1991 Mar 25;19(6):1273–1278. [PMC free article] [PubMed]
  • Beil B, Screaton G, Stamm S. Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing. DNA Cell Biol. 1997 Jun;16(6):679–690. [PubMed]
  • Bennett M, Michaud S, Kingston J, Reed R. Protein components specifically associated with prespliceosome and spliceosome complexes. Genes Dev. 1992 Oct;6(10):1986–2000. [PubMed]
  • Berget SM. Exon recognition in vertebrate splicing. J Biol Chem. 1995 Feb 10;270(6):2411–2414. [PubMed]
  • Blencowe BJ. Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases. Trends Biochem Sci. 2000 Mar;25(3):106–110. [PubMed]
  • Blencowe BJ, Baurén G, Eldridge AG, Issner R, Nickerson JA, Rosonina E, Sharp PA. The SRm160/300 splicing coactivator subunits. RNA. 2000 Jan;6(1):111–120. [PMC free article] [PubMed]
  • Blencowe BJ, Bowman JA, McCracken S, Rosonina E. SR-related proteins and the processing of messenger RNA precursors. Biochem Cell Biol. 1999;77(4):277–291. [PubMed]
  • Blencowe BJ, Issner R, Nickerson JA, Sharp PA. A coactivator of pre-mRNA splicing. Genes Dev. 1998 Apr 1;12(7):996–1009. [PMC free article] [PubMed]
  • Boggs RT, Gregor P, Idriss S, Belote JM, McKeown M. Regulation of sexual differentiation in D. melanogaster via alternative splicing of RNA from the transformer gene. Cell. 1987 Aug 28;50(5):739–747. [PubMed]
  • Bouck J, Fu XD, Skalka AM, Katz RA. Role of the constitutive splicing factors U2AF65 and SAP49 in suboptimal RNA splicing of novel retroviral mutants. J Biol Chem. 1998 Jun 12;273(24):15169–15176. [PubMed]
  • Bourgeois CF, Popielarz M, Hildwein G, Stevenin J. Identification of a bidirectional splicing enhancer: differential involvement of SR proteins in 5' or 3' splice site activation. Mol Cell Biol. 1999 Nov;19(11):7347–7356. [PMC free article] [PubMed]
  • Bruzik JP, Maniatis T. Enhancer-dependent interaction between 5' and 3' splice sites in trans. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):7056–7059. [PMC free article] [PubMed]
  • Burtis KC, Baker BS. Drosophila doublesex gene controls somatic sexual differentiation by producing alternatively spliced mRNAs encoding related sex-specific polypeptides. Cell. 1989 Mar 24;56(6):997–1010. [PubMed]
  • Cáceres JF, Krainer AR. Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains. EMBO J. 1993 Dec;12(12):4715–4726. [PMC free article] [PubMed]
  • Cáceres JF, Screaton GR, Krainer AR. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. Genes Dev. 1998 Jan 1;12(1):55–66. [PMC free article] [PubMed]
  • Cáceres JF, Stamm S, Helfman DM, Krainer AR. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science. 1994 Sep 16;265(5179):1706–1709. [PubMed]
  • Cavaloc Y, Bourgeois CF, Kister L, Stévenin J. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. RNA. 1999 Mar;5(3):468–483. [PMC free article] [PubMed]
  • Cavaloc Y, Popielarz M, Fuchs JP, Gattoni R, Stévenin J. Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family. EMBO J. 1994 Jun 1;13(11):2639–2649. [PMC free article] [PubMed]
  • Champlin DT, Frasch M, Saumweber H, Lis JT. Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin. Genes Dev. 1991 Sep;5(9):1611–1621. [PubMed]
  • Chandler SD, Mayeda A, Yeakley JM, Krainer AR, Fu XD. RNA splicing specificity determined by the coordinated action of RNA recognition motifs in SR proteins. Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3596–3601. [PMC free article] [PubMed]
  • Chew SL, Liu HX, Mayeda A, Krainer AR. Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing. Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10655–10660. [PMC free article] [PubMed]
  • Chiara MD, Reed R. A two-step mechanism for 5' and 3' splice-site pairing. Nature. 1995 Jun 8;375(6531):510–513. [PubMed]
  • Colwill K, Pawson T, Andrews B, Prasad J, Manley JL, Bell JC, Duncan PI. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution. EMBO J. 1996 Jan 15;15(2):265–275. [PMC free article] [PubMed]
  • Côté J, Simard MJ, Chabot B. An element in the 5' common exon of the NCAM alternative splicing unit interacts with SR proteins and modulates 5' splice site selection. Nucleic Acids Res. 1999 Jun 15;27(12):2529–2537. [PMC free article] [PubMed]
  • Crispino JD, Blencowe BJ, Sharp PA. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science. 1994 Sep 23;265(5180):1866–1869. [PubMed]
  • Crispino JD, Sharp PA. A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1-independent splicing. Genes Dev. 1995 Sep 15;9(18):2314–2323. [PubMed]
  • Dauwalder B, Amaya-Manzanares F, Mattox W. A human homologue of the Drosophila sex determination factor transformer-2 has conserved splicing regulatory functions. Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9004–9009. [PMC free article] [PubMed]
  • Dauwalder B, Mattox W. Analysis of the functional specificity of RS domains in vivo. EMBO J. 1998 Oct 15;17(20):6049–6060. [PMC free article] [PubMed]
  • Denhez F, Lafyatis R. Conservation of regulated alternative splicing and identification of functional domains in vertebrate homologs to the Drosophila splicing regulator, suppressor-of-white-apricot. J Biol Chem. 1994 Jun 10;269(23):16170–16179. [PubMed]
  • Diamond RH, Du K, Lee VM, Mohn KL, Haber BA, Tewari DS, Taub R. Novel delayed-early and highly insulin-induced growth response genes. Identification of HRS, a potential regulator of alternative pre-mRNA splicing. J Biol Chem. 1993 Jul 15;268(20):15185–15192. [PubMed]
  • Du C, McGuffin ME, Dauwalder B, Rabinow L, Mattox W. Protein phosphorylation plays an essential role in the regulation of alternative splicing and sex determination in Drosophila. Mol Cell. 1998 Dec;2(6):741–750. [PubMed]
  • Eldridge AG, Li Y, Sharp PA, Blencowe BJ. The SRm160/300 splicing coactivator is required for exon-enhancer function. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6125–6130. [PMC free article] [PubMed]
  • Fetzer S, Lauber J, Will CL, Lührmann R. The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase. RNA. 1997 Apr;3(4):344–355. [PMC free article] [PubMed]
  • Fortes P, Bilbao-Cortés D, Fornerod M, Rigaut G, Raymond W, Séraphin B, Mattaj IW. Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition. Genes Dev. 1999 Sep 15;13(18):2425–2438. [PMC free article] [PubMed]
  • Fu XD. Specific commitment of different pre-mRNAs to splicing by single SR proteins. Nature. 1993 Sep 2;365(6441):82–85. [PubMed]
  • Fu XD. The superfamily of arginine/serine-rich splicing factors. RNA. 1995 Sep;1(7):663–680. [PMC free article] [PubMed]
  • Fu XD, Maniatis T. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature. 1990 Feb 1;343(6257):437–441. [PubMed]
  • Fu XD, Maniatis T. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site. Proc Natl Acad Sci U S A. 1992 Mar 1;89(5):1725–1729. [PMC free article] [PubMed]
  • Fu XD, Maniatis T. Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. Science. 1992 Apr 24;256(5056):535–538. [PubMed]
  • Fu XD, Mayeda A, Maniatis T, Krainer AR. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection. Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11224–11228. [PMC free article] [PubMed]
  • Ge H, Manley JL. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell. 1990 Jul 13;62(1):25–34. [PubMed]
  • Ge H, Zuo P, Manley JL. Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Cell. 1991 Jul 26;66(2):373–382. [PubMed]
  • Golovkin M, Reddy AS. The plant U1 small nuclear ribonucleoprotein particle 70K protein interacts with two novel serine/arginine-rich proteins. Plant Cell. 1998 Oct;10(10):1637–1648. [PMC free article] [PubMed]
  • Golovkin M, Reddy AS. An SC35-like protein and a novel serine/arginine-rich protein interact with Arabidopsis U1-70K protein. J Biol Chem. 1999 Dec 17;274(51):36428–36438. [PubMed]
  • Graveley BR, Hertel KJ, Maniatis T. A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers. EMBO J. 1998 Nov 16;17(22):6747–6756. [PMC free article] [PubMed]
  • Graveley BR, Maniatis T. Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing. Mol Cell. 1998 Apr;1(5):765–771. [PubMed]
  • Gross T, Richert K, Mierke C, Lützelberger M, Käufer NF. Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors. Nucleic Acids Res. 1998 Jan 15;26(2):505–511. [PMC free article] [PubMed]
  • Gui JF, Lane WS, Fu XD. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature. 1994 Jun 23;369(6482):678–682. [PubMed]
  • Gui JF, Tronchère H, Chandler SD, Fu XD. Purification and characterization of a kinase specific for the serine- and arginine-rich pre-mRNA splicing factors. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10824–10828. [PMC free article] [PubMed]
  • Hanes J, von der Kammer H, Klaudiny J, Scheit KH. Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases. J Mol Biol. 1994 Dec 16;244(5):665–672. [PubMed]
  • Hertel KJ, Lynch KW, Maniatis T. Common themes in the function of transcription and splicing enhancers. Curr Opin Cell Biol. 1997 Jun;9(3):350–357. [PubMed]
  • Hertel KJ, Maniatis T. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing. Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2651–2655. [PMC free article] [PubMed]
  • Jamison SF, Pasman Z, Wang J, Will C, Lührmann R, Manley JL, Garcia-Blanco MA. U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: characterization of required elements. Nucleic Acids Res. 1995 Aug 25;23(16):3260–3267. [PMC free article] [PubMed]
  • Johnson KW, Smith KA. Molecular cloning of a novel human cdc2/CDC28-like protein kinase. J Biol Chem. 1991 Feb 25;266(6):3402–3407. [PubMed]
  • Kan JL, Green MR. Pre-mRNA splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor. Genes Dev. 1999 Feb 15;13(4):462–471. [PMC free article] [PubMed]
  • Kanopka A, Mühlemann O, Petersen-Mahrt S, Estmer C, Ohrmalm C, Akusjärvi G. Regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins. Nature. 1998 May 14;393(6681):185–187. [PubMed]
  • Kohtz JD, Jamison SF, Will CL, Zuo P, Lührmann R, Garcia-Blanco MA, Manley JL. Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors. Nature. 1994 Mar 10;368(6467):119–124. [PubMed]
  • Krainer AR, Conway GC, Kozak D. The essential pre-mRNA splicing factor SF2 influences 5' splice site selection by activating proximal sites. Cell. 1990 Jul 13;62(1):35–42. [PubMed]
  • Krainer AR, Conway GC, Kozak D. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells. Genes Dev. 1990 Jul;4(7):1158–1171. [PubMed]
  • Krainer AR, Mayeda A, Kozak D, Binns G. Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell. 1991 Jul 26;66(2):383–394. [PubMed]
  • Labourier E, Bourbon HM, Gallouzi IE, Fostier M, Allemand E, Tazi J. Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development. Genes Dev. 1999 Mar 15;13(6):740–753. [PMC free article] [PubMed]
  • Lavigueur A, La Branche H, Kornblihtt AR, Chabot B. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding. Genes Dev. 1993 Dec;7(12A):2405–2417. [PubMed]
  • Lazar G, Schaal T, Maniatis T, Goodman HM. Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7672–7676. [PMC free article] [PubMed]
  • Li H, Bingham PM. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing. Cell. 1991 Oct 18;67(2):335–342. [PubMed]
  • Li Y, Blencowe BJ. Distinct factor requirements for exonic splicing enhancer function and binding of U2AF to the polypyrimidine tract. J Biol Chem. 1999 Dec 3;274(49):35074–35079. [PubMed]
  • Liu HX, Zhang M, Krainer AR. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev. 1998 Jul 1;12(13):1998–2012. [PMC free article] [PubMed]
  • Liu HX, Chew SL, Cartegni L, Zhang MQ, Krainer AR. Exonic splicing enhancer motif recognized by human SC35 under splicing conditions. Mol Cell Biol. 2000 Feb;20(3):1063–1071. [PMC free article] [PubMed]
  • Longman D, Johnstone IL, Cáceres JF. Functional characterization of SR and SR-related genes in Caenorhabditis elegans. EMBO J. 2000 Apr 3;19(7):1625–1637. [PMC free article] [PubMed]
  • Lopato S, Gattoni R, Fabini G, Stevenin J, Barta A. A novel family of plant splicing factors with a Zn knuckle motif: examination of RNA binding and splicing activities. Plant Mol Biol. 1999 Mar;39(4):761–773. [PubMed]
  • Lopato S, Mayeda A, Krainer AR, Barta A. Pre-mRNA splicing in plants: characterization of Ser/Arg splicing factors. Proc Natl Acad Sci U S A. 1996 Apr 2;93(7):3074–3079. [PMC free article] [PubMed]
  • Lopato S, Waigmann E, Barta A. Characterization of a novel arginine/serine-rich splicing factor in Arabidopsis. Plant Cell. 1996 Dec;8(12):2255–2264. [PMC free article] [PubMed]
  • Lorson CL, Androphy EJ. An exonic enhancer is required for inclusion of an essential exon in the SMA-determining gene SMN. Hum Mol Genet. 2000 Jan 22;9(2):259–265. [PubMed]
  • Lützelberger M, Gross T, Käufer NF. Srp2, an SR protein family member of fission yeast: in vivo characterization of its modular domains. Nucleic Acids Res. 1999 Jul 1;27(13):2618–2626. [PMC free article] [PubMed]
  • Lynch KW, Maniatis T. Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer. Genes Dev. 1996 Aug 15;10(16):2089–2101. [PubMed]
  • Mayeda A, Screaton GR, Chandler SD, Fu XD, Krainer AR. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements. Mol Cell Biol. 1999 Mar;19(3):1853–1863. [PMC free article] [PubMed]
  • Mayeda A, Zahler AM, Krainer AR, Roth MB. Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing. Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1301–1304. [PMC free article] [PubMed]
  • Merendino L, Guth S, Bilbao D, Martínez C, Valcárcel J. Inhibition of msl-2 splicing by Sex-lethal reveals interaction between U2AF35 and the 3' splice site AG. Nature. 1999 Dec 16;402(6763):838–841. [PubMed]
  • Michaud S, Reed R. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev. 1991 Dec;5(12B):2534–2546. [PubMed]
  • Misteli T, Cáceres JF, Clement JQ, Krainer AR, Wilkinson MF, Spector DL. Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo. J Cell Biol. 1998 Oct 19;143(2):297–307. [PMC free article] [PubMed]
  • Misteli T, Cáceres JF, Spector DL. The dynamics of a pre-mRNA splicing factor in living cells. Nature. 1997 May 29;387(6632):523–527. [PubMed]
  • Misteli T, Spector DL. The cellular organization of gene expression. Curr Opin Cell Biol. 1998 Jun;10(3):323–331. [PubMed]
  • Misteli T, Spector DL. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Mol Cell. 1999 Jun;3(6):697–705. [PubMed]
  • Nishii Y, Morishima M, Kakehi Y, Umehara K, Kioka N, Terano Y, Amachi T, Ueda K. CROP/Luc7A, a novel serine/arginine-rich nuclear protein, isolated from cisplatin-resistant cell line. FEBS Lett. 2000 Jan 14;465(2-3):153–156. [PubMed]
  • Ono Y, Ohno M, Shimura Y. Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22. Mol Cell Biol. 1994 Nov;14(11):7611–7620. [PMC free article] [PubMed]
  • Prasad J, Colwill K, Pawson T, Manley JL. The protein kinase Clk/Sty directly modulates SR protein activity: both hyper- and hypophosphorylation inhibit splicing. Mol Cell Biol. 1999 Oct;19(10):6991–7000. [PMC free article] [PubMed]
  • Query CC, Bentley RC, Keene JD. A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein. Cell. 1989 Apr 7;57(1):89–101. [PubMed]
  • Reed R. Initial splice-site recognition and pairing during pre-mRNA splicing. Curr Opin Genet Dev. 1996 Apr;6(2):215–220. [PubMed]
  • Ring HZ, Lis JT. The SR protein B52/SRp55 is essential for Drosophila development. Mol Cell Biol. 1994 Nov;14(11):7499–7506. [PMC free article] [PubMed]
  • Roscigno RF, Garcia-Blanco MA. SR proteins escort the U4/U6.U5 tri-snRNP to the spliceosome. RNA. 1995 Sep;1(7):692–706. [PMC free article] [PubMed]
  • Rossi F, Labourier E, Forné T, Divita G, Derancourt J, Riou JF, Antoine E, Cathala G, Brunel C, Tazi J. Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I. Nature. 1996 May 2;381(6577):80–82. [PubMed]
  • Roth MB, Zahler AM, Stolk JA. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J Cell Biol. 1991 Nov;115(3):587–596. [PMC free article] [PubMed]
  • Ruskin B, Zamore PD, Green MR. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell. 1988 Jan 29;52(2):207–219. [PubMed]
  • Ryner LC, Goodwin SF, Castrillon DH, Anand A, Villella A, Baker BS, Hall JC, Taylor BJ, Wasserman SA. Control of male sexual behavior and sexual orientation in Drosophila by the fruitless gene. Cell. 1996 Dec 13;87(6):1079–1089. [PubMed]
  • Sanford JR, Bruzik JP. Developmental regulation of SR protein phosphorylation and activity. Genes Dev. 1999 Jun 15;13(12):1513–1518. [PMC free article] [PubMed]
  • Schaal TD, Maniatis T. Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA. Mol Cell Biol. 1999 Jan;19(1):261–273. [PMC free article] [PubMed]
  • Schaal TD, Maniatis T. Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. Mol Cell Biol. 1999 Mar;19(3):1705–1719. [PMC free article] [PubMed]
  • Screaton GR, Cáceres JF, Mayeda A, Bell MV, Plebanski M, Jackson DG, Bell JI, Krainer AR. Identification and characterization of three members of the human SR family of pre-mRNA splicing factors. EMBO J. 1995 Sep 1;14(17):4336–4349. [PMC free article] [PubMed]
  • Selvakumar M, Helfman DM. Exonic splicing enhancers contribute to the use of both 3' and 5' splice site usage of rat beta-tropomyosin pre-mRNA. RNA. 1999 Mar;5(3):378–394. [PMC free article] [PubMed]
  • Shi H, Hoffman BE, Lis JT. A specific RNA hairpin loop structure binds the RNA recognition motifs of the Drosophila SR protein B52. Mol Cell Biol. 1997 May;17(5):2649–2657. [PMC free article] [PubMed]
  • Smith CW, Porro EB, Patton JG, Nadal-Ginard B. Scanning from an independently specified branch point defines the 3' splice site of mammalian introns. Nature. 1989 Nov 16;342(6247):243–247. [PubMed]
  • Soret J, Gattoni R, Guyon C, Sureau A, Popielarz M, Le Rouzic E, Dumon S, Apiou F, Dutrillaux B, Voss H, et al. Characterization of SRp46, a novel human SR splicing factor encoded by a PR264/SC35 retropseudogene. Mol Cell Biol. 1998 Aug;18(8):4924–4934. [PMC free article] [PubMed]
  • Spikes DA, Kramer J, Bingham PM, Van Doren K. SWAP pre-mRNA splicing regulators are a novel, ancient protein family sharing a highly conserved sequence motif with the prp21 family of constitutive splicing proteins. Nucleic Acids Res. 1994 Oct 25;22(21):4510–4519. [PMC free article] [PubMed]
  • Sukegawa J, Blobel G. A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor. J Biol Chem. 1995 Jun 30;270(26):15702–15706. [PubMed]
  • Sun Q, Mayeda A, Hampson RK, Krainer AR, Rottman FM. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes Dev. 1993 Dec;7(12B):2598–2608. [PubMed]
  • Tacke R, Chen Y, Manley JL. Sequence-specific RNA binding by an SR protein requires RS domain phosphorylation: creation of an SRp40-specific splicing enhancer. Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1148–1153. [PMC free article] [PubMed]
  • Tacke R, Manley JL. The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities. EMBO J. 1995 Jul 17;14(14):3540–3551. [PMC free article] [PubMed]
  • Tacke R, Manley JL. Determinants of SR protein specificity. Curr Opin Cell Biol. 1999 Jun;11(3):358–362. [PubMed]
  • Tacke R, Tohyama M, Ogawa S, Manley JL. Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell. 1998 Apr 3;93(1):139–148. [PubMed]
  • Tarn WY, Steitz JA. SR proteins can compensate for the loss of U1 snRNP functions in vitro. Genes Dev. 1994 Nov 15;8(22):2704–2717. [PubMed]
  • Tarn WY, Steitz JA. Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps. Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2504–2508. [PMC free article] [PubMed]
  • Teigelkamp S, Mundt C, Achsel T, Will CL, Lührmann R. The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p. RNA. 1997 Nov;3(11):1313–1326. [PMC free article] [PubMed]
  • Tian M, Maniatis T. Positive control of pre-mRNA splicing in vitro. Science. 1992 Apr 10;256(5054):237–240. [PubMed]
  • Tian M, Maniatis T. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA. Cell. 1993 Jul 16;74(1):105–114. [PubMed]
  • Tian M, Maniatis T. A splicing enhancer exhibits both constitutive and regulated activities. Genes Dev. 1994 Jul 15;8(14):1703–1712. [PubMed]
  • Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD. SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J Cell Biol. 1998 Feb 23;140(4):737–750. [PMC free article] [PubMed]
  • Wang J, Manley JL. Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways. RNA. 1995 May;1(3):335–346. [PMC free article] [PubMed]
  • Wang J, Takagaki Y, Manley JL. Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability. Genes Dev. 1996 Oct 15;10(20):2588–2599. [PubMed]
  • Wang J, Xiao SH, Manley JL. Genetic analysis of the SR protein ASF/SF2: interchangeability of RS domains and negative control of splicing. Genes Dev. 1998 Jul 15;12(14):2222–2233. [PMC free article] [PubMed]
  • Wang Z, Hoffmann HM, Grabowski PJ. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity. RNA. 1995 Mar;1(1):21–35. [PMC free article] [PubMed]
  • Wu JY, Maniatis T. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell. 1993 Dec 17;75(6):1061–1070. [PubMed]
  • Wu S, Romfo CM, Nilsen TW, Green MR. Functional recognition of the 3' splice site AG by the splicing factor U2AF35. Nature. 1999 Dec 16;402(6763):832–835. [PubMed]
  • Xiao SH, Manley JL. Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing. Genes Dev. 1997 Feb 1;11(3):334–344. [PubMed]
  • Xiao SH, Manley JL. Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2. EMBO J. 1998 Nov 2;17(21):6359–6367. [PMC free article] [PubMed]
  • Zahler AM, Lane WS, Stolk JA, Roth MB. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 1992 May;6(5):837–847. [PubMed]
  • Zahler AM, Neugebauer KM, Lane WS, Roth MB. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science. 1993 Apr 9;260(5105):219–222. [PubMed]
  • Zahler AM, Neugebauer KM, Stolk JA, Roth MB. Human SR proteins and isolation of a cDNA encoding SRp75. Mol Cell Biol. 1993 Jul;13(7):4023–4028. [PMC free article] [PubMed]
  • Zamore PD, Green MR. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9243–9247. [PMC free article] [PubMed]
  • Zamore PD, Patton JG, Green MR. Cloning and domain structure of the mammalian splicing factor U2AF. Nature. 1992 Feb 13;355(6361):609–614. [PubMed]
  • Zhang M, Zamore PD, Carmo-Fonseca M, Lamond AI, Green MR. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8769–8773. [PMC free article] [PubMed]
  • Zhang WJ, Wu JY. Functional properties of p54, a novel SR protein active in constitutive and alternative splicing. Mol Cell Biol. 1996 Oct;16(10):5400–5408. [PMC free article] [PubMed]
  • Zhou Z, Reed R. Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing. EMBO J. 1998 Apr 1;17(7):2095–2106. [PMC free article] [PubMed]
  • Zorio DA, Blumenthal T. Both subunits of U2AF recognize the 3' splice site in Caenorhabditis elegans. Nature. 1999 Dec 16;402(6763):835–838. [PubMed]
  • Zuo P, Maniatis T. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing. Genes Dev. 1996 Jun 1;10(11):1356–1368. [PubMed]
  • Zuo P, Manley JL. Functional domains of the human splicing factor ASF/SF2. EMBO J. 1993 Dec;12(12):4727–4737. [PMC free article] [PubMed]
  • Zuo P, Manley JL. The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3363–3367. [PMC free article] [PubMed]

Articles from RNA are provided here courtesy of The RNA Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...