Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 2002 Sep; 184(17): 4757–4766.
PMCID: PMC135285

Site-Specific Recombination System Encoded by Toluene Catabolic Transposon Tn4651


The 56-kb class II toluene catabolic transposon Tn4651 from Pseudomonas putida plasmid pWW0 is unique in that (i) its efficient resolution requires, in addition to the 0.2-kb resolution (res) site, the two gene products TnpS and TnpT and (ii) the 2.4-kb tnpT-res-tnpS region is 48 kb apart from the tnpA gene (M. Tsuda, K.-I. Minegishi, and T. Iino, J. Bacteriol. 171:1386-1393, 1989). Detailed analysis of the 2.4-kb region revealed that the tnpS and tnpT genes encoding the putative 323- and 332-amino-acid proteins, respectively, were transcribed divergently with an overlapping 59-bp sequence in the 203-bp res site. The motifs (the R-H-R-Y tetrad in domains I and II with proper spacing) commonly conserved in the integrase family of site-specific recombinases were found in TnpS. In contrast, TnpT did not show any significant amino acid sequence homology to the other proteins that are directly or indirectly involved in recombination. Analysis of site-specific recombination under the Escherichia coli recA cells indicated that (i) the site-specific resolution between the two copies of the res site on a single molecule was catalyzed by TnpS, (ii) the functional res site was located within a 95-bp segment, and (iii) TnpT appeared to have the role of enhancing the site-specific resolution. It was also found that TnpS catalyzed the site-specific recombination between the res sites located at two different molecules to form a cointegrate molecule. Site-specific mutagenesis of the conserved tyrosine residue in TnpS led to the loss of both the resolution and the integration activities, indicating that such a residue took part in both types of recombination.

Many prokaryotic mobile elements have been identified that carry various xenobiotic-degrading genes (33, 38, 41). The most extensively characterized xenobiotic-degrading genes are the toluene-degrading (xyl) genes on plasmid pWW0 from Pseudomonas putida mt-2 (3, 24). We have previously demonstrated that all of the xyl genes on this 117-kb plasmid were located on Tn4651, a 56-kb transposon belonging to the class II family (Fig. (Fig.1)1) (36). In general, one end of the typical class II transposon is occupied by the transposase (tnpA) and resolvase (tnpR) genes and the resolution (res) site, and transposition of such a mobile element occurs by a two-step process (29). In the former step, TnpA recognizes the short (<50 bp) terminal inverted repeats (IRs) and mediates the formation of the cointegrate of the donor and target replicons with two copies of transposon, one at each junction. In the latter step, TnpR catalyzes resolution of the cointegrate by site-specific recombination between the two copies of the res site, giving rise to the donor replicon and the target replicon with an insert of the transposon. Tn4651, however, showed some unique features in the organization of the transposition-related genes (Fig. (Fig.1).1). The Tn4651 tnpA gene located at one end was, contrary to those of other typical transposons, transcribed in an inward direction (13). Furthermore, the efficient site-specific resolution of the Tn4651-mediated cointegrate required the two gene products, TnpS and TnpT, and the 2.4-kb region carrying tnpS-res-tnpT was 48 kb apart from the tnpA gene (34). Although the cointegration step of Tn4651 has been extensively analyzed by Kivisaar's group (13, 14, 15, 32), no detailed analysis of the resolution system has been carried out in more than a decade.

FIG. 1.
Structure of Tn4651. Restriction site abbreviations: A, AatII; Ap, ApaI; B, BamHI; E, EcoRI; H, HindIII; K, KpnI; N, NcoI; Sa, SacI; Sb, SacII; Sm, SmaI; St, StuI; and X, XbaI. Restriction sites derived from the vectors are in parentheses. (A) Schematic ...

Prokaryotic site-specific recombinases can be divided into the two major families, the resolvase and integrase families, on the basis of similarity of the amino acid sequences, conserved motifs, and molecular mechanisms of recombination (23, 29). Most members of the resolvase family represented by the TnpR proteins of the class II transposons catalyze only the intramolecular recombination between the two copies of the recombination site (12). In contrast, almost all of the members of the integrase family represented by the phage-encoded integrases catalyze both the intramolecular and intermolecular recombinations, and this family also includes the recombinases that take part in integration of the gene cassettes into the integrons and their excision (11) and the transposition of conjugative transposons (27, 30). The TnpI proteins encoded by the two unusual class II transposons, Tn4430 and Tn5401, also belong to the integrase family, and they catalyze the site-specific resolution of their respective cointegrates (5, 21). However, it has not been investigated whether these two proteins are also able to catalyze the intermolecular site-specific recombination. Some members of the integrase family require additional accessory proteins for the intramolecular recombination. For instance, excision of lambda phage DNA from its lysogenic chromosome of Escherichia coli requires, in addition to integrase (Int), the phage-encoded excisionase (Xis), as well as the integration host factor (IHF) encoded by the host chromosome (18).

In the present study, we analyzed the Tn4651-encoded site-specific resolution system under E. coli recA backgrounds. We show that (i) TnpS is a site-specific recombinase belonging to the integrase family and catalyzes both intra- and intermolecular site-specific recombinations and (ii) TnpT appears to have a role to enhance the former type of recombination.


Bacterial strains, plasmids, and growth conditions.

The bacterial strains and plasmids used in this study are listed in Table Table1.1. Routine cultivation of E. coli and P. putida cells was performed at 37 and 30°C, respectively. Luria-Bertani (LB) broth was used as a liquid medium and was solidified with 1.5% agar to prepare LB agar plate (7). Final concentrations of the supplements added to media were as follows: ampicillin (AMP), 50 μg/ml; kanamycin, 50 μg/ml; chloramphenicol, 30 μg/ml; streptomycin, 100 μg/ml; tetracycline (TET), 10 μg/ml; nalidixic acid, 20 μg/ml; IPTG (isopropyl-β-d-thiogalactopyranoside), 0.1 mM.

Bacterial strains and plasmids used in this study

DNA manipulations and construction of plasmids.

Established procedures were employed for preparation and manipulation of plasmid DNA, agarose gel electrophoresis, and transformation of E. coli cells (28). PCR was, unless otherwise stated, carried out by using ExTaq DNA polymerase (Takara), and the primer sequences are available upon request. To clone the PCR fragments into appropriate plasmids, the following recognition sites were added in the 5′ parts of the primers: HindIII in S1; EcoRI in S2; ScaI in RES1, RES2, and RES32; and KpnI in RES6 (Fig. (Fig.22 and and3A3A).

FIG. 2.
Construction of pMT299 and R388 derivatives carrying various parts of the res site. Only the relevant restriction sites are depicted, and their abbreviations are as described in the legend of Fig. Fig.11 except for the following: Hc, HincII; P, ...
FIG. 3.
Structure of the Tn4651 res site. (A) Sequence of the res site. The numbers at the left are the base positions obtained by taking the upstream XbaI site (Fig. (Fig.1B)1B) as position 1. The transcription start sites of tnpS and tnpT are indicated ...

Insertion of the 2.4-kb XbaI-SmaI fragment containing the Tn4651 tnpS-res-tnpT region into the corresponding sites of pUC119 gave rise to pGEN1 (Fig. (Fig.1C).1C). The 1.32-kb AatII-SacI fragment containing the tnpT gene was excised from pGEN1 and inserted into the corresponding sites of pGEM5Zf(+) so that the vector-derived ApaI site was located just outside of the AatII site. Excision by ApaI and SacI digestion of the tnpT gene from the resulting plasmid and insertion into the corresponding sites of pBluescript II SK(+) gave rise to the plasmid in which the tnpT gene was flanked by the two KpnI sites. Such a KpnI fragment was inserted into the KpnI site of pUC118 to generate pGEN7, and insertion of the pUC4K-derived, BamHI-flanked kanamycin resistance (Kmr) gene into the BamHI site in pGEN7 led to construction of pGEN7K (Fig. (Fig.1C).1C). Excision of the 1.13-kb NcoI-EcoRI fragment containing the tnpT gene from pGEN1 and its subsequent insertion into corresponding sites just downstream of the trc promoter of pTrc99A generated pGEN21. The DNA fragment containing the tnpS gene (base positions 17 to 1056 in AB077820) (Fig. (Fig.1B)1B) was amplified by PCR by using pGEN1 as a template and primers S1 and S2. The PCR product was digested by EcoRI and HindIII and inserted into the corresponding sites of pKK223-3 that are located just downstream of the tac promoter. The resulting plasmid was designated pGEN59 (Fig. (Fig.1C).1C). pGEN77 was constructed by excision of the BamHI-HindIII fragment containing the tac-tnpS fragment from pGEN59 and its insertion into corresponding sites of pGEN21.

In order to change the tyrosine residue of TnpS to phenylalanine, the megaprimer PCR method (6) was employed for site-directed mutagenesis of tnpS by using KOD DNA Polymerase (Toyobo). The first PCR was carried out with the primers S1 and SA179T and pGEN59 as a template (Fig. (Fig.1C).1C). The amplified fragment was used as the megaprimer for the second PCR with S2 as another primer. The amplicon containing the mutant tnpS gene was digested by EcoRI and HindIII and inserted into the corresponding sites of pKK223-3 to obtain pGEN95 (Fig. (Fig.1C).1C). Excision of the BamHI-HindIII fragment containing the tac promoter and the mutant tnpS gene from pGEN95 and its subsequent insertion into the corresponding sites of pGEN21 gave rise to pGEN96.

Determination of transcription start site by primer extension.

Plasmid pMT1405 (pWW0::kan) (35) was introduced into P. putida PT280 (Table (Table1)1) that was shown not to carry the Tn4651 tnpS-res-tnpT region (unpublished data). An RNeasy Mini Kit (Qiagen) was employed to prepare the total RNA from 1-ml samples of LB cultures of PT280 and PT280(pMT1405). A primer extension experiment in a 20-μl reaction volume was performed according to the protocol of Aloka, Ltd. (Tokyo, Japan), with the IRD-41-labeled oligonucleotide primer PEtnpS or PEtnpT (Fig. (Fig.1B).1B). (The primer sequences are available upon request.) Gel electrophoresis was carried out by using a Li-Cor 4000 automated DNA sequencer.

Site-specific recombination assays.

To analyze the site-specific resolution of Tn4651 in this study, a pACYC184-based plasmid, pMT299 (Table (Table1),1), was used to construct its derivative carrying the pUC4K-derived Kmr gene that was flanked by two copies of the res site or its part (Fig. (Fig.2).2). JM109 cells carrying the resulting plasmid were subjected to transformation of the pMB1-based plasmid that carried tnpS, tnpT, or both genes whose expression was under the control of the tac or trc promoter. The resulting transformant cells were briefly cultivated in LB medium with or without the addition of IPTG and then plated on LB agar plates containing appropriate antibiotics. The colonies thus obtained were examined for sensitivity to kanamycin and for the profiles of the residing plasmids. Excision of the circular DNA form consisting of the res site and the Kmr gene was also examined by PCR with primers 4Km1 and 4Km2 (Fig. (Fig.66).

FIG. 6.
PCR detection of site-specific resolution. (A) Schematic drawing for PCR detection of the excised and nonreplicating DNA consisting of the res site and Kmr gene. White arrowheads indicate the primers. (B) Agarose gel electrophoresis of the PCR-amplified ...

To investigate the site-specific integration activity of the Tn4651-specified resolution system, a DNA fragment containing one copy of the res site, together with the Kmr gene, was inserted into conjugative plasmid R388 (Fig. (Fig.2).2). The resulting plasmid was introduced into the DH5α cells that had contained the pMT299 derivative carrying one copy of the res site and the pMB1-based plasmid carrying tnpS, tnpT, or both genes. The strain harboring these three plasmids was employed as the donor to mate with HB101 on a membrane filter (34), and the TET resistance-streptomycin resistance (Tcr Smr) transconjugants were selected to detect the mobilization of the pMT299-based replicon. Such transconjugants were examined for the physical analysis of the plasmids.

Determination and analysis of nucleotide sequence.

The DNA fragments cloned on pUC18 and pUC19 were sequenced with an ABI 310 automated DNA sequencer (Applied Biosystems) according to the protocols recommended by the manufacturer. The nucleotide sequence reported here has been deposited in the DDBJ-EMBL-GenBank database under accession number AB077820. The computer analysis of the sequence was performed with the software programs GENETYX 10 (SDC, Inc., Tokyo, Japan) and BLAST 2 (National Institute of Genetics, Mishima, Japan).


Sequence and expression analysis of tnpS-res-tnpT region.

The 2,368-bp sequence of the Tn4651 XbaI-SmaI fragment encoding its resolution system (34) was determined in this study (accession number AB077820) (Fig. (Fig.1B).1B). The 203-bp sequence (nucleotides from position 1045 [AatII site] to position 1247 [SacII site]) containing the res site is shown in Fig. Fig.3A.3A. The 1,247-bp XbaI-SacII fragment had the two candidate open reading frames (ORFs) for tnpS: the one located at positions 358 to 1032 and the other located at positions 1056 to 88 on the complementary strand. Since deletion of the StuI fragment from positions 225 to 363 had led to loss of the TnpS activity (34), the latter ORF encoding a 323-amino-acid protein was concluded to be tnpS. The 1,324-bp AatII-SmaI fragment had the three candidate ORFs for tnpT: the ORF from positions 1237 to 2232; the ORF from positions 1481 to 1888; and the ORF from positions 1931 to 2182. The first ORF encoding a 332-amino-acid protein was postulated to be tnpT because the DNA fragment covering at least positions 1247 to 2018 (SacII and StuI sites, respectively) had been indicated to be required for TnpT activity (34).

BLAST searches revealed that TnpS had homology with the integrase family of site-specific recombinases (23), showing 36 to 38% identity and 53 to 55% similarity with the putative integrase from the atrazine catabolic plasmid pADP-1 of a Pseudomonas strain (U66917) (22), the integrase-like protein from Coxiella burnetii (Y15898), and the OrfI protein of Tn5041 (X98999) (17). The proteins in this family invariably have the conserved R-H-R-Y tetrad in domains I and II with proper spacing (23), and these structural characteristics were also found in TnpS (Fig. (Fig.4).4). In contrast, TnpT showed 29% identity and 47% similarity only with the OrfQ protein of Tn5041 (X98999) (17).

FIG. 4.
Comparison of TnpS with other members in the integrase family of site-specific recombinases. The consensus sequences in this family are depicted at the top of the figure; the invariant R-H-R-Y tetrad residues in the family are indicated by the capital ...

The transcription start sites of tnpS and tnpT were determined in the P. putida background by the primer extension method (data not shown), and these two genes were found to be transcribed divergently with an overlapping 59-bp sequence in the 203-bp res site (Fig. (Fig.3A).3A). On the basis of this information, the putative promoter sequences of tnpS and tnpT and the putative ribosome-binding (Shine-Dalgarno [SD]) sequence of tnpT are shown in Fig. Fig.3A.3A. However, we could find no putative SD sequences for tnpS. The tnpS gene was inserted just downstream of the tac promoter and its associated SD sequence of pKK223-3 to construct pGEN59, and the tnpT gene was inserted just downstream of the trc promoter and its associated SD sequence of pTrc99A to construct pGEN21 (Fig. (Fig.1C).1C). The addition of 0.1 mM IPTG to the E. coli JM109(pGEN21) cells led to very severe growth inhibition, while such treatment of the JM109(pGEN59) cells did not. The mutant derivatives of JM109(pGEN21) were isolated that could grow normally in the presence of IPTG, and they were categorized into the three groups. The first group had the insertion of an unknown sequence(s) in tnpT, and the second one had the defects in the trc promoter, resulting in no transcription of tnpT. The last group has been suggested to have an unknown chromosomal mutation(s) since transformation of the new JM109 cells by the plasmids prepared from the mutants still produced growth inhibition in the host cells.

Site-specific resolution activity of tnpS-res-tnpT region.

To analyze the Tn4651-encoded resolution system under an E. coli recA background, a pACYC184-based Tcr Kmr plasmid, pGEN101, was constructed in which a Kmr gene was flanked by the two copies of the 203-bp res site (res1) (Fig. (Fig.22 and and3B).3B). We also constructed pGEN77 by insertion of the tac-tnpS region of pGEN59 into pGEN21 that had the trc-tnpT gene (Fig. (Fig.1C).1C). We thereafter introduced pGEN21, pGEN59, or pGEN77 into JM109(pGEN101). Since the prolonged cultivation of JM109(pGEN101)(pGEN21) that overexpressed TnpT alone, but not the two other strains, on LB plates containing 0.1 mM IPTG led to the very severe growth defect, the three JM109 derivatives were cultivated only for 1 h at 37°C in AMP- and TET-containing LB medium with or without IPTG. After such treatment, the cells were plated on LB agar plates containing AMP and TET, and the 150 colonies obtained from each experiment were examined for resistance to kanamycin. Cultivation of JM109(pGEN101)(pGEN21) and JM109(pGEN101)(pGEN59), even in the presence of IPTG, generated no kanamycin-sensitive (Kms) colonies, and physical analysis of the residing plasmids (Fig. (Fig.5A,5A, lanes 1 and 2) revealed that the structure of pGEN101 remained unaffected. In contrast, JM109(pGEN101)(pGEN77) led to formation of the Kms colonies at frequencies of 6 or 9% when the cells were cultivated in the absence or presence, respectively, of IPTG. The addition of IPTG did not lead to a drastic increase in the frequency of formation of the Kms colonies for unknown reasons. The leaky transcription from the trc and tac promoters in the absence of IPTG (20) might have allowed expression of TnpS and TnpT sufficient for the resolution. The Kms derivatives contained, in addition to pGEN77, a novel plasmid (pGEN101R) that was smaller than pGEN101 (Fig. (Fig.5A,5A, lane 4). Analysis of pGEN101R by restriction digestion and sequencing confirmed that this plasmid was formed by site-specific resolution, indicating that both tnpS and tnpT were required for efficient resolution. pGEN101R was also detected in the cleared lysate prepared from the Kmr colonies of JM109(pGEN101)(pGEN77) after their prolonged cultivation in LB containing AMP and TET (Fig. (Fig.5A5A lane 3), indicating that pGEN101R was gradually generated from pGEN101 by site-specific resolution.

FIG. 5.
Resolution and cointegration of plasmids carrying Tn4651 res site. (A) Resolution of plasmid that carried the Kmr gene flanked by two directly repeated copies of the res site. The JM109 derivative carrying the substrate and helper plasmids was cultivated ...

Similar resolution experiments were also carried out by using pGEN101-related plasmids, pGEN102 and pGEN103, that carried the left 160-bp (res2) and 130-bp (res3) sequences, respectively, of res1 (Fig. (Fig.22 and and3B).3B). In the presence of pGEN77, pGEN102 but not pGEN103 resolved site specifically to generate the Kms colonies at a frequency of 5 or 9% in the absence or presence, respectively, of IPTG. The Kms derivatives carried pGEN102R (Fig. (Fig.5A,5A, lane 6) that was the resolved product of pGEN102. Prolonged cultivation of the Kmr cells of JM109(pGEN77)(pGEN103) in LB medium containing AMP and TET did not generate the resolved derivative of pGEN103 (Fig. (Fig.5A,5A, lane 7). These results indicated that (i) the rightmost 73-bp sequence of res1 was not required for efficient resolution and (ii) the rightmost 30-bp sequence of res2 was important for resolution.

To investigate whether the excision of the Kmr gene was correlated with formation of the circular DNA form consisting of the Kmr gene and one copy of the res site, PCR detection of such excision events was performed with the primers 4Km1 and 4Km2 (Fig. (Fig.6A).6A). Use of the cleared lysates from the Kmr cells of JM109(pGEN101)(pGEN77) and JM109(pGEN102)(pGEN77), but not from those of JM109(pGEN103)(pGEN77), led to successful amplification of the DNA molecules with the expected sizes (Fig. (Fig.6B,6B, lanes 3, 7, and 8). These results also supported the conclusion that loss of the Kmr gene occurred by its site-specific excision. The excision events were also detected by PCR from the cleared lysates prepared from JM109(pGEN101)(pGNE59) (Fig. (Fig.6B,6B, lane 2) and JM109(pGEN102)(pGNE59) (data not shown), although these two strains did not generate Kms derivatives (<0.6%). Therefore, TnpT was not certainly required for site-specific resolution but might play a role in enhancing the frequency of site-specific resolution.

The 95-bp sequence covering the rightmost portion of res2 (positions 1110 to 1204 in Fig. Fig.3A)3A) was designated res6, and we constructed a pGEN101-related plasmid, pGEN106, in which the Kmr gene was flanked by two copies of res6 (Fig. (Fig.22 and and3B).3B). The JM109(pGEN106)(pGEN77) strain did not generate Kms derivatives (<0.6%), and the resolved plasmid derived from pGEN106 was not detected when the cleared lysate prepared from the Kmr cells was analyzed by agarose gel electrophoresis (Fig. (Fig.5A,5A, lane 8). However, PCR analysis of the cleared lysate (Fig. (Fig.6,6, lane 9) supported the formation of the excised circular DNA consisting of the Kmr gene and a copy of res6. PCR analysis of the cleared lysate prepared from JM109(pGEN106)(pGEN59) led to the same observation (data not shown). The right end of res6 (Fig. (Fig.3B)3B) had the IR of a 9-bp sequence with a 2-bp spacer and a putative consensus sequence for the binding site of the E. coli IHF protein (9).

Site-specific integration activity of Tn4651-specified recombination system.

Since TnpS had the motif commonly conserved in the integrase family of site-specific recombinases (Fig. (Fig.4),4), we anticipated that TnpS alone or in concert with TnpT might be able to mediate site-specific integration of a res-carrying plasmid into another plasmid that also carried the res site. We expected that such a recombination event could be detected effectively by conjugal mobilization of the nontransmissible plasmid by the coresiding transmissible plasmid only when both plasmids carried the functional res site. pGEN101R that carried res1 and the Tcr gene was employed as the former type of plasmid, and pGEN388 (Fig. (Fig.2)2) was constructed as the latter type of plasmid by insertion of res1 and the Kmr gene into transmissible plasmid R388 (4). The DH5α derivative carrying pGEN101R, pGEN388, and one of the pMB1-based plasmids (pGEN21, pGEN59, or pGEN77) was used as the donor to mate with HB101, and mobilization of pGEN101R was examined by selecting Tcr Smr transconjugants (Table (Table2).2). When we used pTrc99A that did not carry tnpS or tnpT, pGEN101R was mobilized at a frequency of ca. 10−6 per pGEN388 transfer, and each Tcr transconjugant carried a single species of plasmid (data not shown) that could revolve into the two parental plasmids in the presence of pGEN77. Restriction analysis of the fused plasmid and sequence analysis of its res1-containing regions confirmed that the plasmid was formed by reciprocal recombination between the two copies of res1. Since neither tnpS nor tnpT was involved in the unexpected recombination, we did not further investigate the mechanism for such a low-frequency recombination event. Supply of TnpT also resulted in such a low-frequency mobilization of pGEN101R. A >1,000-fold increase in mobilization of this plasmid was observed when TnpS alone and when TnpS and TnpT simultaneously were supplied. The transconjugants thus obtained carried a single species of plasmid (Fig. (Fig.5B)5B) whose structure was the same as that residing in the transconjugants that were obtained by use of DH5α(pGEN101R)(pGEN388)(pTrc99A) as the donor. These results indicated that TnpS alone could efficiently promote site-specific integration without involvement of TnpT. Use of res2 and res6 on the two plasmids, pGEN389 and pMT299res6 (Fig. (Fig.22 and and3B),3B), respectively, also generated the cointegrate (Table (Table2),2), indicating that res2, and most probably res6, was sufficient for site-specific integration.

Site-specific integration mediated by Tn4651 res sitea

Importance of tyrosine residue of TnpS in site-specific recombination.

As described above, TnpS had the R-H-R-Y tetrad commonly conserved in the integrase family of recombinases, and the tyrosine residue in this tetrad in this family has been demonstrated to be a catalytic center of the recombination reaction (23). To investigate whether this residue in TnpS was essential for the site-specific recombination, its tyrosine residue at position 293 (Fig. (Fig.4)4) was changed to phenylalanine by site-directed mutagenesis. The tnpS mutant versions of pGEN59 and pGEN77 were constructed and designated pGEN95 and pGEN96, respectively (Fig. (Fig.1C).1C). Use of these mutant plasmids in the site-specific resolution and integration assays revealed that the mutant TnpS protein lost both the activities (Fig. (Fig.5,5, lane 6; Fig. Fig.6,6, lanes 5 and 6; and Table Table2),2), indicating that the tyrosine residue played an essential role in both types of site-specific recombination.


To investigate the Tn4651-encoded site-specific resolution system in this study, we constructed E. coli recA strains harboring the two compatible plasmids: one carrying the Kmr gene flanked by two copies of the res site or its parts and the other carrying tnpS, tnpT, or both genes (Fig. (Fig.1C,1C, 2, and 3B). Investigation of site-specific resolution by detection of the Kms colonies revealed that the 160-bp res2 sequence contained the functional cis-acting site. Based on this assay system, res6 that corresponded to the rightmost 95-bp portion of res2 was considered not to be functional. However, res6 was indicated to be functional according to more sensitive but qualitative detection of the excised DNA molecule by PCR (Fig. (Fig.6B,6B, lane 9). The 65-bp sequence present on res2 but absent in res6 (Fig. (Fig.3)3) might be important to some extent for effective resolution of the cointegrate. The right end of res6 was occupied by the 9-bp IRs (Fig. (Fig.3).3). In many site-specific recombination systems, such IRs but with different sequences have been demonstrated to be indispensable for efficient binding of the recombinases and/or their accessory proteins to catalyze recombination (40), and the 9-bp IR in res6 of Tn4651 might have a similar role.

The res2 sequence, and most probably the res6 one, could act as the substrate for intramolecular resolution and intermolecular integration reactions, and analysis in this study revealed that (i) both reactions could be catalyzed by TnpS that had the structural properties characteristic of the integrase family and (ii) TnpT was most likely to enhance the former type of reactions. The site-specific recombination system of Tn4651 could therefore be considered to be apparently analogous to those encoded by integrons in which the integrases are able to catalyze both the excision and the integration reactions and, to a lesser extent, analogous to those encoded by lysogenic phages and some conjugative transposons (e.g., Tn916 and Tn5276) (18, 25, 30). With respect to the latter group of mobile elements, the small (<100 amino acid residues) accessory proteins collectively designated recombination direction factors (RDFs) (e.g., lambda Xis protein) (19) are, in addition to the integrases, required absolutely for site-specific excision, and some RDFs have been shown to bind to specific recognition sequences at the att sites so as to introduce sharp DNA bends (8). However, TnpT was much larger than RDFs and did not show homology to any RDFs. It is at present unknown whether TnpT also has the ability to bind to the Tn4651 res site since our attempt to overproduce the TnpT protein alone in E. coli led to a very toxic effect on the growth of the host cells. Our observation that such a toxic effect could most probably be suppressed by the chromosomal but uncharacterized mutation(s) indicated that TnpT might interact with the unknown host product(s). Identification of the mutated chromosomal gene(s) might provide us with some clues to elucidate the molecular mechanism of TnpT in the resolution. There is also a possibility that TnpT interacts directly with TnpS to enhance the resolution frequencies. Such possible titration of the free form of TnpT by TnpS might be able to account for our observation that the attempt to overexpress both TnpS and TnpT simultaneously in E. coli resulted in a less toxic effect on the host cells. Site-specific recombination between the two res sites, one on the pACYC184 replicon and the other on the R388 replicon, was detected at a low frequency without the involvement of TnpS or TnpT (Table (Table2).2). Construction of the latter R388 derivative was associated with disruption of the site-specific integrase gene of In3, an integron on R388 (10). Such a low-frequency integration event might therefore be most likely ascribed to the activity of an unknown integrase protein encoded by the host E. coli chromosome. However, BLAST searches of the protein databases did not clarify the candidate proteins that displayed similarity with TnpS.

A 15-kb mercury transposon, Tn5041 (Fig. (Fig.1A),1A), from a Pseudomonas strain (17) is closely related to Tn4651 in that (i) these two transposons share >95% identity in the genes and sites involved in cointegration (i.e., tnpA and terminal IRs) and (ii) the Tn4651 tnpA product could catalyze the cointegration of Tn5041 (16, 17). The 2.3-kb region of Tn5041 probably responsible for the cointegrate resolution is separated by 9 kb from the tnpAC genes, and this 2.3-kb region carries the putative res site flanked by the two genes, orfI and orfQ, that are postulated to be transcribed divergently (17). Furthermore, the sizes of orfI, orfQ, and the res site of Tn5041 are similar to those of the tnpS, tnpT, and res site, respectively, of Tn4651, and OrfI that possesses the motifs conserved in the integrase family has been speculated to be the counterpart of TnpS. However, the resolution-related region of Tn5041 was not so homologous as to hybridize with the corresponding region of Tn4651 by Southern analysis (17). The Tn5041-encoded resolution system remains to be elucidated since the resolution activity of Tn5041 assayed by a system similar to ours was very weak in the E. coli cells (16). An analysis of the Tn5041-encoded resolution system in P. putida and further investigation of the functional exchangeability of TnpS and OrfI and of TnpT and OrfQ will uncover more clearly the degree of relatedness between the two transposons.


We thank Y. Kamio for the use of the apparatus for primer extension analysis in his laboratory.

This work was supported by a grant-in-aid from the Ministry of Education, Science, Sports, and Culture of Japan.


1. Amann, E., J. Brosius, and M. Ptashne. 1983. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25:167-178. [PubMed]
2. Amann, E., B. Ochs, and K. J. Abel. 1988. Tightly regulated tac promoter vectors useful for the expression of unfused and fused protein in Escherichia coli. Gene 69:301-315. [PubMed]
3. Assinder, S. J., and P. A. Williams. 1990. The TOL plasmids: determinants of the catabolism of toluene and the xylenes. Adv. Microb. Physiol. 31:1-69. [PubMed]
4. Avila, P., and F. de la Cruz. 1988. Physical and genetic mapping of the IncW plasmid R388. Plasmid 20:155-157. [PubMed]
5. Baum, J. A. 1995. TnpI recombinase: identification of sites within Tn5401 required for TnpI binding and site-specific recombination. J. Bacteriol. 177:4036-4042. [PMC free article] [PubMed]
6. Brøns-Poulsen, J., N. E. Petersen, M. Hørder, and K. Kristiansen. 1998. An improved PCR-based method for site-directed mutagenesis using megaprimers. Mol. Cell. Probes 12:345-348. [PubMed]
7. Davis, L., M. Kuehl, and J. Battey. 1994. Basic methods in molecular biology, 2nd ed. Appleton and Lange, East Norwalk, Conn.
8. Franz, B., and A. Landy. 1995. The Holliday junction intermediates of lambda integrative and excisive recombination respond differently to the bending proteins integration host factor and excisionase. EMBO J. 14:397-406. [PMC free article] [PubMed]
9. Friedman, D. I. 1988. Integration host factor: a protein for all reasons. Cell 55:545-554. [PubMed]
10. Hall, R. M., H. J. Brown, D. E. Brookes, and H. W. Stokes. 1994. Integrons found in different locations have identical 5′ ends but variable 3′ ends. J. Bacteriol. 176:6286-6294. [PMC free article] [PubMed]
11. Hall, R. M., and C. M. Collis. 1995. Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. Mol. Microbiol. 15:593-600. [PubMed]
12. Hallet, B., and D. J. Sherratt. 1997. Transposition and site-specific recombination: adapting DNA cut-and-paste mechanisms to a variety of genetic rearrangements. FEMS Microbiol. Rev. 21:157-178. [PubMed]
13. Hõrak, R., and M. Kivisaar. 1998. Expression of the transposase gene tnpA of Tn4652 is positively affected by integration host factor. J. Bacteriol. 180:2822-2829. [PMC free article] [PubMed]
14. Hõrak, R., and M. Kivisaar. 1999. Regulation of the transposase of Tn4652 by the transposon-encoded protein TnpC. J. Bacteriol. 181:6312-6318. [PMC free article] [PubMed]
15. Ilves, H., R. Hõrak, and M. Kivisaar. 2001. Involvement of σS in starvation-induced transposition of Pseudomonas putida transposon Tn4652. J. Bacteriol. 183:5445-5448. [PMC free article] [PubMed]
16. Kholodii, G. I., S. Z. Mindlin, Z. M. Gorlenko, I. A. Bass, E. S. Kaliaeva, and V. G. Nikiforov. 2000. Host-dependent transposition of Tn5041. Russian J. Genet. 36:459-469.
17. Kholodii, G., O. V. Yurieva, Z. M. Gorlenko, S. Z. Mindlin, I. A. Bass, O. L. Lomovskaya, A. V. Kopteva, and V. G. Nikiforov. 1997. Tn5041: a chimeric mercury resistance transposon closely related to the toluene degradative transposon Tn4651. Microbiology 143:2549-2556. [PubMed]
18. Landy, A. 1989. Dynamic, structural, and regulatory aspects of lambda site-specific recombination. Annu. Rev. Biochem. 58:913-949. [PubMed]
19. Lewis, J. A., and G. F. Hatfull. 2001. Control of directionality in integrase-mediated recombination: examination of recombination directionality factors (RDFs) including Xis and Cox proteins. Nucleic Acids Res. 29:2205-2216. [PMC free article] [PubMed]
20. Lowe, C. A., A. H. Asghar, G. Shalom, J. G. Shaw, and M. S. Thomas. 2001. The Burkholderia cepacia fur gene: colocalization with omlA and absence of regulation by iron. Microbiology 147:1303-1314. [PubMed]
21. Mahillon, J., and D. Lereclus. 1988. Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process. EMBO J. 7:1515-1526. [PMC free article] [PubMed]
22. Martinez, B., J. Tomkins, L. P. Wackett, R. Wing, and M. J. Sadowsky. 2001. Complete nucleotide sequence and organization of the atrazine catabolic plasmid pADP-1 from Pseudomonas sp. strain ADP. J. Bacteriol. 183:5684-5697. [PMC free article] [PubMed]
23. Nunes-Düby, S. E., H. J. Kwon, R. S. Tirumalai, T. Ellenberger, and A. Landy. 1998. Similarities and differences among 105 members of the Int family of site-specific recombinases. Nucleic Acids Res. 26:391-406. [PMC free article] [PubMed]
24. Ramos, J. L., S. Marqués, and K. N. Timmis. 1997. Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators. Annu. Rev. Microbiol. 51:341-372. [PubMed]
25. Rauch, P. J., and W. M. de Vos. 1994. Identification and characterization of genes involved in excision of the Lactococcus lactis conjugative transposon Tn5276. J. Bacteriol. 176:2165-2171. [PMC free article] [PubMed]
26. Reddy, B. R., L. E. Shaw, J. R. Sayers, and P. A. Williams. 1994. Two identical copies of IS1246, a 1,275-base-pair sequence related to other bacterial insertion sequences, enclose the xyl genes on TOL plasmid pWW0. Microbiology 140:2305-2307. [PubMed]
27. Salyers, A. A., N. B. Shoemaker, A. M. Stevens, and L. Y. Li. 1995. Conjugative transposons: an unusual and diverse set of integrated gene transfer elements. Microbiol. Rev. 59:579-590. [PMC free article] [PubMed]
28. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
29. Sherratt, D. 1989. Tn3 and related transposable elements: site-specific recombination and transposition, p. 163-184. In D. E. Berg and M. M. Howe (ed.), Mobile DNA. American Society for Microbiology, Washington, D.C.
30. Taylor, K. L., and G. Churchward. 1997. Specific DNA cleavage mediated by the integrase of conjugative transposon Tn916. J. Bacteriol. 179:1117-1125. [PMC free article] [PubMed]
31. Taylor, L. A., and R. E. Rose. 1988. A correction in the nucleotide sequence of the Tn903 kanamycin resistance determinant in pUC4K. Nucleic Acids Res. 16:358. [PMC free article] [PubMed]
32. Teras, R., R. Hõrak, and M. Kivisaar. 2000. Transcription from fusion promoters generated during transposition of transposon Tn4652 is positively affected by integration host factor in Pseudomonas putida. J. Bacteriol. 182:589-598. [PMC free article] [PubMed]
33. Tsuda, M., and H. Genka. 2001. Identification and characterization of Tn4656, a novel class II transposon carrying a set of toluene-degrading genes from TOL plasmid pWW53. J. Bacteriol. 183:6215-6224. [PMC free article] [PubMed]
34. Tsuda, M., and T. Iino. 1987. Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWW0. Mol. Gen. Genet. 210:270-276. [PubMed]
35. Tsuda, M., and T. Iino. 1988. Identification and characterization of Tn4653, a transposon covering the toluene transposon Tn4651 on TOL plasmid pWW0. Mol. Gen. Genet. 213:72-77. [PubMed]
36. Tsuda, M., K.-I. Minegishi, and T. Iino. 1989. Toluene transposons Tn4651 and Tn4653 are class II transposons. J. Bacteriol. 171:1386-1393. [PMC free article] [PubMed]
37. Tsuda, M., and T. Nakazawa. 1993. A mutagenesis system utilizing a Tn1722 derivative containing an Escherichia coli-specific vector plasmid: application to Pseudomonas species. Gene 136:257-262. [PubMed]
38. Tsuda, M., H. M. Tan, A. Nishi, and K. Furukawa. 1999. Mobile catabolic genes in bacteria. J. Biosci. Bioeng. 87:401-410. [PubMed]
39. Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259-268. [PubMed]
40. Williams, K. P. 2002. Integration sites for genetic elements in prokaryotic tRNA and tmRNA genes: sublocation preference of integrase subfamilies. Nucleic Acids Res. 30:866-875. [PMC free article] [PubMed]
41. Wyndham, R. C., A. E. Cashore, C. H. Nakatsu, and M. C. Peel. 1994. Catabolic transposons. Biodegradation 5:323-342. [PubMed]
42. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp 18 and pUC19 vectors. Gene 33:103-119. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)
PubReader format: click here to try


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • BioProject
    BioProject links
  • Compound
    PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
  • Gene
    Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
  • Gene (nucleotide)
    Gene (nucleotide)
    Records in Gene identified from shared sequence and PMC links.
  • MedGen
    Related information in MedGen
  • Nucleotide
    Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
  • Protein
    Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...