A discrimination between two successively alternating stimuli was trained under conditions that maintained equal frequencies of reinforcement in the presence of each of the discriminative stimuli (S1 and S2) but that also reduced the rate of responding to S2. These conditions included a multiple variable-interval differential-reinforcement-of-low-rate schedule and a multiple variable-interval variable-interval schedule in which responses to S2 were punished. Whenever the rate of responding to S2 was reduced, rate of responding to S1 (behavioral contrast) increased, and the peak of a subsequently obtained generalization gradient did not occur at the expected location (between S1 and S2) but was displaced away from S2, below S1. Discrimination training in which the frequencies of reinforcement earned in S1 and S2 were not equal (variable-interval 1-min variable-interval 5-min training) produced contrast and the peak shift only if the rate of responding to S2 had been reduced, as after non-differential reinforcement in which variable-interval 1-min schedules were correlated with S1 and with S2. It was concluded that a sufficient condition for the occurrence of behavioral contrast and the peak shift was reduction of the rate of responding to one of two alternating discriminative stimuli and that a peak shift will occur only if contrast had occurred during discrimination training.