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Biophys J. 1975 Mar; 15(3): 191–203.
PMCID: PMC1334617

Light scattering from nucleated biological cells.


The light scattered from nucleated biological cells has been investigated by using four different theoretical models: an opaque disk, a homogeneous sphere, an opaque ring, and a coated sphere. By comparing these four models, diffraction at the edges of the cell and the nucleus has been found to be the predominate scattering mechanism for nucleated biological cells at low angles. The scattering patterns of nucleated cells are found to have a fine lobe (high-frequency) structure dependent on whole cell size, and an envelope lobe (low-frequency) structure dependent on relative nucleus size. The models indicate that the present technique for measuring cell size with a single low-angle light detector is highly dependent on the nucleus to cell diameter ratio. Whole cell size is better estimated by the ratio of the outputs from two low-angle detectors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brunsting A. Can light-scattering techniques be applied to flow-through cell analysis? J Histochem Cytochem. 1974 Jul;22(7):607–615. [PubMed]
  • Mullaney PF, Van Dilla MA, Coulter JR, Dean PN. Cell sizing: a light scattering photometer for rapid volume determination. Rev Sci Instrum. 1969 Aug;40(8):1029–1032. [PubMed]
  • Mullaney PF, Dean PN. The small angle light scattering of biological cells. Theoretical considerations. Biophys J. 1970 Aug;10(8):764–772. [PMC free article] [PubMed]
  • Brunsting A, Mullaney PF. Differential light scattering from spherical mammalian cells. Biophys J. 1974 Jun;14(6):439–453. [PMC free article] [PubMed]
  • Steinkamp JA, Fulwyler MJ, Coulter JR, Hiebert RD, Horney JL, Mullancy PF. A new multiparameter separator for microscopic particles and biological cells. Rev Sci Instrum. 1973 Sep;44(9):1301–1310. [PubMed]

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