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Nucleotide sequence of penA gene of N. gonorrhoeae. The sequences of the penA genes of penicillin-susceptible strain LM306 (GenBank accession no. M32091) (A), the strain with reduced susceptibility to cefixime (strain NG-3) (B), and cefixime-susceptible strain NG-12 (C) are shown. The insertion of an extra aspartate (Asp-345A) is shown in cefixime-susceptible strain NG-12 but is not shown in the strain with reduced susceptibility to cefixime, NG-3. Asp∗, Asp-345A. The Ser-X-X-Lys, Ser-X-Asn, and Lys-Thr-Gly conserved motifs are indicated by underlining.

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