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Biophys J. Mar 2000; 78(3): 1087–1093.
PMCID: PMC1300712

How yeast cells synchronize their glycolytic oscillations: a perturbation analytic treatment.

Abstract

Of all the lifeforms that obtain their energy from glycolysis, yeast cells are among the most basic. Under certain conditions the concentrations of the glycolytic intermediates in yeast cells can oscillate. Individual yeast cells in a suspension can synchronize their oscillations to get in phase with each other. Although the glycolytic oscillations originate in the upper part of the glycolytic chain, the signaling agent in this synchronization appears to be acetaldehyde, a membrane-permeating metabolite at the bottom of the anaerobic part of the glycolytic chain. Here we address the issue of how a metabolite remote from the pacemaking origin of the oscillation may nevertheless control the synchronization. We present a quantitative model for glycolytic oscillations and their synchronization in terms of chemical kinetics. We show that, in essence, the common acetaldehyde concentration can be modeled as a small perturbation on the "pacemaker" whose effect on the period of the oscillations of cells in the same suspension is indeed such that a synchronization develops.

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Selected References

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  • Astumian RD, Chock PB, Tsong TY, Westerhoff HV. Effects of oscillations and energy-driven fluctuations on the dynamics of enzyme catalysis and free-energy transduction. Phys Rev A. 1989 Jun 15;39(12):6416–6435. [PubMed]
  • BETZ A, CHANCE B. PHASE RELATIONSHIP OF GLYCOLYTIC INTERMEDIATES IN YEAST CELLS WITH OSCILLATORY METABOLIC CONTROL. Arch Biochem Biophys. 1965 Mar;109:585–594. [PubMed]
  • Bier M, Teusink B, Kholodenko BN, Westerhoff HV. Control analysis of glycolytic oscillations. Biophys Chem. 1996 Nov 29;62(1-3):15–24. [PubMed]
  • Cortassa S, Aon MA, Westerhoff HV. Linear nonequilibrium thermodynamics describes the dynamics of an autocatalytic system. Biophys J. 1991 Oct;60(4):794–803. [PMC free article] [PubMed]
  • Ghosh AK, Chance B, Pye EK. Metabolic coupling and synchronization of NADH oscillations in yeast cell populations. Arch Biochem Biophys. 1971 Jul;145(1):319–331. [PubMed]
  • Goldbeter A, Lefever R. Dissipative structures for an allosteric model. Application to glycolytic oscillations. Biophys J. 1972 Oct;12(10):1302–1315. [PMC free article] [PubMed]
  • Heinrich R, Rapoport SM, Rapoport TA. Metabolic regulation and mathematical models. Prog Biophys Mol Biol. 1977;32(1):1–82. [PubMed]
  • Hess B, Boiteux A. Mechanism of glycolytic oscillation in yeast. I. Aerobic and anaerobic growth conditions for obtaining glycolytic oscillation. Hoppe Seylers Z Physiol Chem. 1968 Nov;349(11):1567–1574. [PubMed]
  • Hess B, Mikhailov A. Self-organization in living cells. Science. 1994 Apr 8;264(5156):223–224. [PubMed]
  • Kacser H, Burns JA. The control of flux. Symp Soc Exp Biol. 1973;27:65–104. [PubMed]
  • Kamp F, Astumian RD, Westerhoff HV. Coupling of vectorial proton flow to a biochemical reaction by local electric interactions. Proc Natl Acad Sci U S A. 1988 Jun;85(11):3792–3796. [PMC free article] [PubMed]
  • Keulers M, Suzuki T, Satroutdinov AD, Kuriyama H. Autonomous metabolic oscillation in continuous culture of Saccharomyces cerevisiae grown on ethanol. FEMS Microbiol Lett. 1996 Sep 1;142(2-3):253–258. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Martinez de la Fuente I, Martinez L, Veguillas J. Dynamic behavior in glycolytic oscillations with phase shifts. Biosystems. 1995;35(1):1–13. [PubMed]
  • Richard P, Bakker BM, Teusink B, Van Dam K, Westerhoff HV. Acetaldehyde mediates the synchronization of sustained glycolytic oscillations in populations of yeast cells. Eur J Biochem. 1996 Jan 15;235(1-2):238–241. [PubMed]
  • Richard P, Diderich JA, Bakker BM, Teusink B, van Dam K, Westerhoff HV. Yeast cells with a specific cellular make-up and an environment that removes acetaldehyde are prone to sustained glycolytic oscillations. FEBS Lett. 1994 Mar 21;341(2-3):223–226. [PubMed]
  • Richard P, Teusink B, Hemker MB, Van Dam K, Westerhoff HV. Sustained oscillations in free-energy state and hexose phosphates in yeast. Yeast. 1996 Jun 30;12(8):731–740. [PubMed]
  • Richard P, Teusink B, Westerhoff HV, van Dam K. Around the growth phase transition S. cerevisiae's make-up favours sustained oscillations of intracellular metabolites. FEBS Lett. 1993 Feb 22;318(1):80–82. [PubMed]
  • Richter O, Betz A, Giersch C. The response of oscillating glycolysis to perturbations in the NADH/NAD system: a comparison between experiments and a computer model. Biosystems. 1975 Jul;7(1):137–146. [PubMed]
  • Sel'kov EE. Stabilization of energy charge, generation of oscillations and multiple steady states in energy metabolism as a result of purely stoichiometric regulation. Eur J Biochem. 1975 Nov 1;59(1):151–157. [PubMed]
  • Termonia Y, Ross J. Oscillations and control features in glycolysis: numerical analysis of a comprehensive model. Proc Natl Acad Sci U S A. 1981 May;78(5):2952–2956. [PMC free article] [PubMed]
  • Teusink B, Bakker BM, Westerhoff HV. Control of frequency and amplitudes is shared by all enzymes in three models for yeast glycolytic oscillations. Biochim Biophys Acta. 1996 Jul 31;1275(3):204–212. [PubMed]
  • Teusink B, Larsson C, Diderich J, Richard P, van Dam K, Gustafsson L, Westerhoff HV. Synchronized heat flux oscillations in yeast cell populations. J Biol Chem. 1996 Oct 4;271(40):24442–24448. [PubMed]
  • Westerhoff HV, Tsong TY, Chock PB, Chen YD, Astumian RD. How enzymes can capture and transmit free energy from an oscillating electric field. Proc Natl Acad Sci U S A. 1986 Jul;83(13):4734–4738. [PMC free article] [PubMed]
  • Wolf J, Heinrich R. Dynamics of two-component biochemical systems in interacting cells; synchronization and desynchronization of oscillations and multiple steady states. Biosystems. 1997;43(1):1–24. [PubMed]

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