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Biophys J. 1998 December; 75(6): 3179–3183. | PMCID: PMC1299989 |
Temperature transitions of protein properties in human red blood cells. G M Artmann, C Kelemen, D Porst, G Büldt, and S Chien Department of Cell Biophysics, Aachen University of Applied Sciences, Ginsterweg 1, D-52428 Jülich, Germany. artmann@fh-aachen.de Human red blood cells (RBC) undergo a sudden change from blocking to passing through 1.3 +/- 0.2-micrometer micropipettes at a transition temperature (Tc) of 36.4 degrees C. For resealed RBC ghosts this transition occurs at 28.3 degrees C (Tg). These findings are attributed to an elastomeric transition of hemoglobin from being gel-like to a fluid and to an elastomeric transition of membrane proteins such as spectrin. Spectrin shows a uniform distribution along the aspirated RBC tongue above Tg in contrast to the linear gradient below Tg. The Full Text of this article is available as a PDF (119K). These references are in PubMed. This may not be the complete list of references from this article. - Artmann GM. Microscopic photometric quantification of stiffness and relaxation time of red blood cells in a flow chamber. Biorheology. 1995 Sep–Oct;32(5):553–570. [PubMed]
- Artmann GM, Sung KL, Horn T, Whittemore D, Norwich G, Chien S. Micropipette aspiration of human erythrocytes induces echinocytes via membrane phospholipid translocation. Biophys J. 1997 Mar;72(3):1434–1441. [PMC free article] [PubMed]
- Bloom JA, Webb WW. Lipid diffusibility in the intact erythrocyte membrane. Biophys J. 1983 Jun;42(3):295–305. [PMC free article] [PubMed]
- Chien S, Sung KL, Skalak R, Usami S, Tözeren A. Theoretical and experimental studies on viscoelastic properties of erythrocyte membrane. Biophys J. 1978 Nov;24(2):463–487. [PMC free article] [PubMed]
- Discher DE, Mohandas N. Kinematics of red cell aspiration by fluorescence-imaged microdeformation. Biophys J. 1996 Oct;71(4):1680–1694. [PMC free article] [PubMed]
- Discher DE, Mohandas N, Evans EA. Molecular maps of red cell deformation: hidden elasticity and in situ connectivity. Science. 1994 Nov 11;266(5187):1032–1035. [PubMed]
- DODGE JT, MITCHELL C, HANAHAN DJ. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. [PubMed]
- Evans EA. Structure and deformation properties of red blood cells: concepts and quantitative methods. Methods Enzymol. 1989;173:3–35. [PubMed]
- Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215–226. [PubMed]
- Golan DE, Brown CS, Cianci CM, Furlong ST, Caulfield JP. Schistosomula of Schistosoma mansoni use lysophosphatidylcholine to lyse adherent human red blood cells and immobilize red cell membrane components. J Cell Biol. 1986 Sep;103(3):819–828. [PMC free article] [PubMed]
- Hochmuth RM, Buxbaum KL, Evans EA. Temperature dependence of the viscoelastic recovery of red cell membrane. Biophys J. 1980 Jan;29(1):177–182. [PMC free article] [PubMed]
- Jay AW. Viscoelastic properties of the human red blood cell membrane. I. Deformation, volume loss, and rupture of red cells in micropipettes. Biophys J. 1973 Nov;13(11):1166–1182. [PMC free article] [PubMed]
- Maksymiw R, Sui SF, Gaub H, Sackmann E. Electrostatic coupling of spectrin dimers to phosphatidylserine containing lipid lamellae. Biochemistry. 1987 Jun 2;26(11):2983–2990. [PubMed]
- Mohandas N, Chasis JA. Red blood cell deformability, membrane material properties and shape: regulation by transmembrane, skeletal and cytosolic proteins and lipids. Semin Hematol. 1993 Jul;30(3):171–192. [PubMed]
- Mohandas N, Evans E. Mechanical properties of the red cell membrane in relation to molecular structure and genetic defects. Annu Rev Biophys Biomol Struct. 1994;23:787–818. [PubMed]
- Nash GB, Meiselman HJ. Alteration of red cell membrane viscoelasticity by heat treatment: effect on cell deformability and suspension viscosity. Biorheology. 1985;22(1):73–84. [PubMed]
- Nash GB, Meiselman HJ. Effects of preparative procedures on the volume and content of resealed red cell ghosts. Biochim Biophys Acta. 1985 May 28;815(3):477–485. [PubMed]
- Palek J, Curby WA, Lionetti FJ. Effects of calcium and adenosine triphosphate on volume of human red cell ghosts. Am J Physiol. 1971 Jan;220(1):19–26. [PubMed]
- Scott MD, Kuypers FA, Butikofer P, Bookchin RM, Ortiz OE, Lubin BH. Effect of osmotic lysis and resealing on red cell structure and function. J Lab Clin Med. 1990 Apr;115(4):470–480. [PubMed]
- Sung KL, Chien S. Influence of temperature on rheology of human erythrocytes. Chin J Physiol. 1992;35(2):81–94. [PubMed]
- Urry DW. Entropic elastic processes in protein mechanisms. I. Elastic structure due to an inverse temperature transition and elasticity due to internal chain dynamics. J Protein Chem. 1988 Feb;7(1):1–34. [PubMed]
- Vertessy BG, Steck TL. Elasticity of the human red cell membrane skeleton. Effects of temperature and denaturants. Biophys J. 1989 Feb;55(2):255–262. [PMC free article] [PubMed]
- Waugh R, Evans EA. Thermoelasticity of red blood cell membrane. Biophys J. 1979 Apr;26(1):115–131. [PMC free article] [PubMed]
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