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Biochem J. Aug 1967; 104(2): 534–536.3.
PMCID: PMC1270616

Purification and amino acid composition of monomeric and polymeric collagens

Abstract

The preparations and amino acid compositions of highly purified tropocollagen and insoluble polymerized collagen are described. These collagens appear to be very suitable for comparative studies in an investigation of the cross-linkages that are introduced into tropocollagen during the formation of polymerized collagen.

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Selected References

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  • Blumenfeld OO, Gallop PM. Amino aldehydes in tropocollagen: the nature of a probable cross-link. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1260–1267. [PMC free article] [PubMed]
  • BORNSTEIN P, PIEZ KA. COLLAGEN: STRUCTURAL STUDIES BASED ON THE CLEAVAGE OF METHIONYL BONDS. Science. 1965 Jun 4;148(3675):1353–1355. [PubMed]
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  • PIEZ KA, MORRIS L. A modified procedure for the automatic analysis of amino acids. Anal Biochem. 1960 Nov;1:187–201. [PubMed]
  • STEVEN FS. THE NISHIHARA TECHNIQUE FOR THE SOLUBILIZATION OF COLLAGEN. APPLICATION TO THE PREPARATION OF SOLUBLE COLLAGENS FROM NORMAL AND RHEUMATOID CONNECTIVE TISSUE. Ann Rheum Dis. 1964 Jul;23:300–301. [PMC free article] [PubMed]
  • STEVEN FS, TRISTRAM GR. The presence of non-protein nitrogen in acetic acid-soluble calf-skin collagen. Biochem J. 1962 May;83:240–244. [PMC free article] [PubMed]
  • Veis A, Anesey J. Modes of intermolecular cross-linking in mature insoluble collagen. J Biol Chem. 1965 Oct;240(10):3899–3908. [PubMed]

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