Logo of biochemjBJ Latest papers and much more!
Biochem J. Jan 1967; 102(1): 65–75.
PMCID: PMC1270211

The action of aflatoxin B1 on the rat liver

Abstract

The administration of a single dose of aflatoxin B1 to the rat (7mg./kg. body wt.) results in the slow development of a periportal necrosis. Hepatic enzymes are released into the serum in the second 24hr. of the poisoning, closely preceding the onset of the necrosis, which is followed by a rise in serum alkaline-phosphatase activity and bilirubin concentration. Aflatoxin B1 has been detected in the nucleus of the poisoned liver cell and in vitro it has been shown to interact with DNA. The toxin inhibits the production of nuclear RNA, probably by preventing the transcription of DNA by the RNA polymerase. It is proposed that the interaction of the toxin with DNA gives rise to its inhibitory action on mitosis and its necrogenic action.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.6M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bernhard W, Frayssinet C, Lafarge C, Le Breton E. Lésions nucléolaires précoces provoquées par l'aflatoxine dans les cellules hépatiques du rat. C R Acad Sci Hebd Seances Acad Sci D. 1965 Aug 18;261(7):1785–1788. [PubMed]
  • Breuer CB, Davis FF. Limited action of actinomycin D on protein synthesis in adrenalectomized rats. Biochem Biophys Res Commun. 1964;14:215–219. [PubMed]
  • BUTLER WH. ACUTE TOXICITY OF AFLATOXIN B-1 IN RATS. Br J Cancer. 1964 Dec;18:756–762. [PMC free article] [PubMed]
  • Butler WH, Clifford JI. Extraction of aflatoxin from rat liver. Nature. 1965 Jun 5;206(988):1045–1046. [PubMed]
  • DAVIDSON JN, FRAZER SC, HUTCHISON WC. Phosphorus compounds in the cell. I. Protein-bound phosphorus fractions studied with the aid of radioactive phosphorus. Biochem J. 1951 Aug;49(3):311–321. [PMC free article] [PubMed]
  • De Recondo AM, Frayssinet C, Lafarge C, Le Breton E. Inhibition de la synthèse du DNA par l'aflatoxine B-1 au cours de l'hypertrophie compensatrice du foie chez le rat. C R Acad Sci Hebd Seances Acad Sci D. 1965 Aug 2;261(5):1409–1412. [PubMed]
  • EMMELOT P, BENEDETTI EL. Changes in the fine structure of rat liver cells brought about by dimethylnitrosamine. J Biophys Biochem Cytol. 1960 Apr;7:393–396. [PMC free article] [PubMed]
  • FARBER E, SHULL KH, VILLA-TREVINO S, LOMBARDI B, THOMAS M. BIOCHEMICAL PATHOLOGY OF ACUTE HEPATIC ADENOSINETRIPHOSPHATE DEFICIENCY. Nature. 1964 Jul 4;203:34–40. [PubMed]
  • FEIGELSON P, GREENGARD O. A microsomal iron-porphyrin activator of rat liver tryptophan pyrrolase. J Biol Chem. 1961 Jan;236:153–157. [PubMed]
  • FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed]
  • GREENBAUM AL, SLATER TF. Studies on the particulate components of rat mammary gland. II. Changes in the levels of the nucleic acids of the mammary glands of rats during pregnancy, lactation and mammary involution. Biochem J. 1957 May;66(1):155–161. [PMC free article] [PubMed]
  • GREENGARD O, SMITH MA, ACS G. Relation of cortisone and synthesis of ribonucleic acid to induced and developmental enzyme formation. J Biol Chem. 1963 Apr;238:1548–1551. [PubMed]
  • HACKMANN C. Untersuchungen über den Einfluss des Sanamycins (Actinomycin C) auf tierische Organe: Milz, Thymus, Lymphknoten, Nebennieren und Keimdrüsen. Z Krebsforsch. 1954;60(2):250–255. [PubMed]
  • Haslewood GA, King EJ. The estimation of bilirubin in blood plasma. Biochem J. 1937 Jun;31(6):920–923. [PMC free article] [PubMed]
  • JUDAH JD, WILLIAMS-ASHMAN HG. The inhibition of oxidative phosphorylation. Biochem J. 1951 Jan;48(1):33–42. [PMC free article] [PubMed]
  • Legator MS, Zuffante SM, Harp AR. Aflatoxin: effect on cultured heteroploid human embryonic lung cells. Nature. 1965 Oct 23;208(5008):345–347. [PubMed]
  • Lilly LJ. Induction of chromosome aberrations by aflatoxin. Nature. 1965 Jul 24;207(995):433–434. [PubMed]
  • MANCHESTER KL, YOUNG FG. The effect of insulin in vitro on the accumulation of amino acids by isolated rat diaphragm. Biochem J. 1960 Jun;75:487–495. [PMC free article] [PubMed]
  • NESBITT BF, O'KELLY J, SARGEANT K, SHERIDAN A. Aspergillus flavus and turkey X disease. Toxic metabolites of Aspergillus flavus. Nature. 1962 Sep 15;195:1062–1063. [PubMed]
  • PHILIPS FS, SCHWARTZ HS, STERNBERG SS, TAN CT. The toxicity of actinomycin D. Ann N Y Acad Sci. 1960 Oct 5;89:348–360. [PubMed]
  • RECKNAGEL RO, ANTHONY DD. Biochemical changes in carbon tetrachloride fatty liver: separation of fatty changes from mitochondrial degeneration. J Biol Chem. 1959 May;234(5):1052–1059. [PubMed]
  • REES KR, ROWLAND GF. The metabolism of isolated rat-liver nuclei. Biochem J. 1961 Jan;78:89–95. [PMC free article] [PubMed]
  • REES KR, SHOTLANDER VL. CHANGES IN LIPID METABOLISM DURING THE DEVELOPMENT OF THE FATTY LIVER. Br J Exp Pathol. 1964 Aug;45:364–374. [PMC free article] [PubMed]
  • REES KR, SINHA KP. Blood enzymes in liver injury. J Pathol Bacteriol. 1960 Oct;80:297–307. [PubMed]
  • REES KR, SPECTOR WG. Reversible nature of liver cell damage due to carbon tetrachloride as demonstrated by the use of phenergan. Nature. 1961 May 27;190:821–822. [PubMed]
  • RENDI R, HULTIN T. Preparation and amino acid incorporating ability of ribonucleoprotein-particles from different tissues of the rat. Exp Cell Res. 1960 Mar;19:253–266. [PubMed]
  • Schimke RT, Sweeney EW, Berlin CM. An analysis of the kinetics of rat liver tryptophan pyrrolase induction: the significance of both enzyme synthesis and degradation. Biochem Biophys Res Commun. 1964 Mar 26;15(3):214–219. [PubMed]
  • SCHWARTZ HS, SODERGREN JE, GAROFALO M, STERNBERG SS. ACTINOMYCIN D. EFFECTS ON NUCLEIC ACID AND PROTEIN METABOLISM IN INTACT AND REGENERATING LIVER OF RATS. Cancer Res. 1965 Apr;25:307–317. [PubMed]
  • SMUCKLER EA, BENDITT EP. STUDIES ON CARBON TETRACHLORIDE INTOXICATION. 3. A SUBCELLULAR DEFECT IN PROTEIN SYNTHESIS. Biochemistry. 1965 Apr;4:671–679. [PubMed]
  • Sporn MB, Dingman CW, Phelps HL, Wogan GN. Aflatoxin B1: binding to DNA in vitro and alteration of RNA metabolism in vivo. Science. 1966 Mar 25;151(3717):1539–1541. [PubMed]
  • STAEHELIN T, WETTSTEIN FO, NOLL H. Breakdown of rat-liver ergosomes in vivo after actinomycin inhibition of messenger RNA synthesis. Science. 1963 Apr 12;140(3563):180–183. [PubMed]
  • Theron JJ. Acute liver injury in ducklings as a result of aflatoxin poisoning. Lab Invest. 1965 Sep;14(9):1586–1603. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...