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Biochem J. Sep 1966; 100(3): 754–761.
PMCID: PMC1265211

Separation and partial purification of the protein hormones from human pituitary glands

Abstract

A method has been developed for separation and partial purification of four protein hormones from acetone-dried human pituitary glands. The major portion of growth hormone, follicle-stimulating hormone, luteinizing hormone and thyroid-stimulating hormone activities are each obtained in a separate fraction. Although the yields of the individual hormones are similar to those obtained by other investigators, the present procedure is simpler and a cleaner separation of the hormones has been achieved. Methods for further purification of each fraction are indicated including preparation of luteinizing hormone of very high potency (equivalent to 5·3mg. of standard NIH–LH–S3/mg.).

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Selected References

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  • BAKKE JL. ASSAY OF HUMAN THYROID-STIMULATING HORMONE BY 18 DIFFERENT ASSAY LABORATORIES USING 12 DIFFERENT METHODS. J Clin Endocrinol Metab. 1965 Apr;25:545–550. [PubMed]
  • BATES RW, CONDLIFFE PG. Studies on the chemistry and bioassay of thyrotropins from bovine pituitaries, transplantable pituitary tumors of mice, and blood plasma. Recent Prog Horm Res. 1960;16:309–352. [PubMed]
  • BUTT WR, CROOKE AC, CUNNINGHAM FJ. Studies on human urinary and pituitary gonadotrophins. Biochem J. 1961 Dec;81:596–605. [PMC free article] [PubMed]
  • BUTT WR, CROOKE AC, CUNNINGHAM FJ, EVANS AJ. Chemical reactions which affect the biological activity of human gonadotrophins. Biochem J. 1961 Apr;79:64–70. [PMC free article] [PubMed]
  • BUTT WR, CUNNINGHAM FJ, HARTREE AS. GONADOTROPHINS. PREPARATION AND ASSAY OF HUMAN PITUITARY FSH AND LH. Proc R Soc Med. 1964 Feb;57:107–108. [PMC free article] [PubMed]
  • CONDLIFFE PG. Purification of human thyrotrophin. Endocrinology. 1963 Jun;72:893–896. [PubMed]
  • EHRLICH RM, RANDLE PJ. Immunoassay of growth hormone in human serum. Lancet. 1961 Jul 29;2(7196):230–233. [PubMed]
  • HARTREE AS, BUTT WR, KIRKHAM KE. THE SEPARATION AND PURIFICATION OF HUMAN LUTEINIZING AND THYROTROPHIC HORMONES. J Endocrinol. 1964 Apr;29:61–69. [PubMed]
  • Hartree AS, Kovacić N, Thomas M. Growth-promoting and luteotrophic activities of human growth hormone. J Endocrinol. 1965 Oct;33(2):249–258. [PubMed]
  • KIRKHAM KE. A new bioassay technique for the measurement in vitro of thyrotrophic hormone in serum and in pituitary extracts. J Endocrinol. 1962 Oct;25:259–269. [PubMed]
  • PARLOW AF, CONDLIFFE PG, REICHERT LE, Jr, WILHELMI AE. RECOVERY AND PARTIAL PURIFICATION OF FSH AND LH DURING THE PURIFICATION OF TSH FROM HUMAN PITUITARY GLANDS. Endocrinology. 1965 Jan;76:27–34. [PubMed]
  • Parlow AF, Wilhelmi AE, Reichert LE., Jr Further studies on the fractionation of human pituitary glands. Endocrinology. 1965 Dec;77(6):1126–1134. [PubMed]
  • READ CH, BRYAN GT. The immunological assay of human growth hormone. Recent Prog Horm Res. 1960;16:187–218. [PubMed]
  • REICHERT LE, Jr, PARLOW AF. PARTIAL PURIFICATION AND SEPARATION OF HUMAN PITUITARY GONADOTROPHINS. Endocrinology. 1964 Feb;74:236–243. [PubMed]
  • REICHERT LE, Jr, PARLOW AF. FURTHER STUDIES ON THE PURIFICATION OF HUMAN PITUITARY LUTEINIZING HORMONES. Endocrinology. 1964 Nov;75:815–817. [PubMed]
  • STEELMAN SL, SEGALOFF A, ANDERSEN RN. Purification of human pituitary follicle stimulating (FSH) and luteinizing (LH) hormones. Proc Soc Exp Biol Med. 1959 Jul;101(3):452–454. [PubMed]
  • STEELMAN SL, SEGALOFF A, MAYS MG. Preparation of highly active human gonadotropin and growth hormone. Arch Biochem Biophys. 1958 Nov;78(1):262–263. [PubMed]
  • WILHELMI AE. Fractionation of human pituitary glands. Can J Biochem Physiol. 1961 Nov;39:1659–1668. [PubMed]

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