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EMBO J. Sep 3, 2001; 20(17): 4753–4761.
PMCID: PMC125265

NRG1, a repressor of filamentous growth in C.albicans, is down-regulated during filament induction


In response to a variety of external signals, the fungal pathogen Candida albicans undergoes a transition between ellipsoidal single cells (blastospores) and filaments composed of elongated cells attached end-to-end. Here we identify a DNA-binding protein, Nrg1, that represses filamentous growth in Candida probably by acting through the co-repressor Tup1. nrg1 mutant cells are predominantly filamentous under non-filament-inducing conditions and their colony morphology resembles that of tup1 mutants. We also identify two filament-specific genes, ECE1 and HWP1, whose transcription is repressed by Nrg1 under non-inducing conditions. These genes constitute a subset of those under Tup1 control, providing further evidence that Nrg1 acts by recruiting Tup1 to target genes. We show that growth in serum at 37°C, a potent inducer of filamentous growth, causes a reduction of NRG1 mRNA, suggesting that filamentous growth is induced by the down-regulation of NRG1. Consistent with this idea, expression of NRG1 from a non-regulated promoter partially blocks the induction of filamentous growth.

Keywords: Candida albicans/filaments/hyphae/Nrg1/Tup1


The yeast Candida albicans has been recognized as a human pathogen for over a century. Although C.albicans is found as a commensal organism present in the human digestive tract, this yeast is also the most common human fungal pathogen, causing oral and vaginal thrush, as well as more serious mucosal and systemic infections in immunocompromised individuals (Odds, 1988, 1994a,b; Dupont, 1995; Weig et al., 1998). Candida albicans can thrive in the bloodstream and on mucosal surfaces; during systemic disease it can infect all the major internal organs. Candida albicans infections are especially serious in AIDS patients, patients undergoing chemotherapy, transplantation patients undergoing immunosuppression therapy and recipients of artificial joints and other prosthetic devices (for reviews see Shepherd et al., 1985; Dupont, 1995; Weig et al., 1998). Despite its importance as a pathogen, relatively little is known about C.albicans when compared with ‘model’ yeasts such as Saccharomyces cerevisiae and Schizosaccharomyces pombe.

What features of C.albicans make it a human pathogen? It seems likely that many of the several thousand C.albicans genes that are not present in S.cerevisiae will turn out to be involved with the interaction between C.albicans and its mammalian hosts (Stanford DNA Sequencing and Technology Center, http://www-sequence. stanford.edu/group/candida). Even many of the gene products that are shared between S.cerevisiae and C.albicans appear to be put to somewhat different uses; for example signaling pathways and receptors in one organism may respond to different environmental signals and produce different responses in the other organism. Because of the many differences between S.cerevisiae and C.albicans and because C.albicans has been adapted to grow exclusively in warm-blooded animal hosts, the term virulence factor is difficult to define rigorously for C.albicans. Nonetheless, work of the past 10 years has identified several features of C.albicans that are required for its virulence (as measured in a simple mouse tail-vein injection model), but which are not essential for growth in laboratory media. These include the capacity of C.albicans to grow both in a single-celled yeast form (the blastospore) and in a spectrum of filamentous forms, adhesiveness to host cells, and secretion of degradative enzymes such as proteases and phospholipases (for reviews see Odds, 1994a; Corner and Magee, 1997; Kobayashi and Cutler, 1998; Madhani and Fink, 1998; Mitchell, 1998; Brown and Gow, 1999).

In this study, we identify and characterize a gene that controls one of these virulence factors, the transition between the blastospore and filamentous forms of C.albicans. This transition is regulated by many different environmental stimuli including serum, nitrogen levels, pH and temperature (for recent reviews see Mitchell, 1998; Brown and Gow, 1999; Ernst, 2000). Some of the evidence that this transition is important for virulence derives from experiments showing that a mutant of C.albicans defective in filament formation (a Δcph1/Δcph1 Δefg1/Δefg1 double mutant) is avirulent (Lo et al., 1997). Likewise, a mutant that is locked into the filamentous forms (a Δtup1/Δtup1 strain) is also avirulent (Braun and Johnson, 1997; Braun et al., 2000). Moreover, a mixture of these two strains is also avirulent (our unpublished observations). In the simplest view, these experiments, taken together, suggest that the transition between the blastospore and filamentous forms is required for virulence. However, the mutations used in these experiments produce multiple phenotypes, and it is not possible at this point definitively to ascribe the lack of virulence of these strains solely to a defect in the blastospore–filament transition.

The transcriptional repressor Tup1 is a key regulator of filamentous growth in C.albicans, as deletion of this gene causes constitutive filamentous growth, in addition to other, more subtle phenotypes (Braun and Johnson, 1997). Deletion of Tup1 from S.cerevisiae results in viable cells that display a remarkable variety of phenotypes, including flocculence, loss of cell type control in α cells, inability to sporulate, enhanced dTMP uptake, temperature sensitivity and loss of glucose repression (Lemontt et al., 1980; Williams and Trumbly, 1990; Keleher et al., 1992; Tzamarias and Struhl, 1994; Treitel and Carlson, 1995; Mizuno et al., 1998; reviewed by Smith and Johnson, 2000). As far as is known, each specific phenotype is based on the loss of Tup1-mediated repression of a set of genes that normally is activated only under specific environmental or internal conditions. (One exception has been reported, however, where the Tup1–Ssn6 complex may cause activation rather than repression; Conlan et al., 1999.) Tup1 does not bind directly to the promoters of these target genes, but rather is brought to DNA by specific DNA-binding proteins each of which controls a set of co-regulated genes. In S.cerevisiae, 10 DNA-binding proteins that repress through Tup1 are known: Mig1, Mig2, Rox1, α2/Mcm1, Rfx1, a1/α2, Sko1 and Nrg1 (Keleher et al., 1992; Deckert et al., 1995b; Treitel and Carlson, 1995; Tzamarias and Struhl, 1995; Huang et al., 1998; Lutfiyya et al., 1998; Park et al., 1999; Proft and Serrano, 1999; reviewed by Smith and Johnson, 2000). Others, such as those responsible for flocculation, have not yet been described. The pleiotropic phenotypes of the tup1 mutant result from derepression of all of these sets of genes, and subsets of the tup1 phenotype can be created by the deletion of one or more of these DNA-binding proteins.

The fact that Tup1 is involved in filamentous growth and virulence in C.albicans does not provide specific information about the targets of repression or the signals that relieve it. It does, however, predict the existence of one or more DNA-binding proteins that bring Tup1 to DNA and thereby negatively regulate genes involved in filamentous growth and virulence. The work from S.cerevisiae also predicts that the regulation of these genes is brought about by modification of these DNA-binding proteins rather than by changes in Tup1 itself (Lutfiyya et al., 1998; Treitel et al., 1998; for reviews see Johnston, 1999; Smith and Johnson, 2000). Here, we report the identification and characterization of one such DNA-binding protein, C.albicans Nrg1, which appears to account for a large part of Tup1’s role in filamentous growth. Several lines of evidence suggest a model whereby filamentous growth is induced through down-regulation of the Nrg1 repressor.


Identification of C.albicans NRG1

To identify DNA-binding proteins that confer Tup1 repression in C.albicans, we searched the partially completed C.albicans genome sequence for sequences similar to those of known TUP1-dependent DNA-binding repressors in S.cerevisiae. Sequence data for C.albicans were obtained from the Stanford DNA Sequencing and Technology Center website at http://www-sequence. stanford.edu/group/candida. Based on this information, we deleted from C.albicans close relatives of the S.cerevisiae genes MIG1 (contig no. 4-2700), SKO1 (contig no. 4-2301) and NRG1 (contig no. 4-3083), and assessed their phenotypes with respect to filamentous growth. Candida albicans is diploid, and both copies of the indicated genes were deleted for this analysis. Deletion of the C.albicans NRG1 relative produced dramatically increased filamentous growth compared with the parent strain (Figure 2), whereas deletion of MIG1 or SKO1 relatives did not produce any immediately obvious phenotypes (data not shown). The C.albicans NRG1 gene is predicted to encode a 310 amino acid, 34 kDa sequence-specific DNA-binding protein of the zinc finger category (Figure 1). Saccharomyces cerevisiae has two closely related Nrg genes, Nrg1 and Nrg2. Nrg1 represses target genes in the presence of glucose, and the function of Nrg2 is not yet known (Park et al., 1999; see Discussion). The C.albicans Nrg1 DNA-binding domain (60 amino acids) is slightly more similar to that of Nrg2 (68% identity) than that of Nrg1 (61% identity). However, the C.albicans protein had no significant sequence similarity to either of the S.cerevisiae proteins outside its DNA-binding domain. The C.albicans gene had no other relatives in the C.albicans sequence database, so it seems likely that there is only one representative of the Nrg proteins in this organism. The near completion of the Candida sequencing project has so far confirmed this conclusion. Based on this information, we refer to the C.albicans gene as NRG1. The fact that the two S.cerevisiae proteins are more related to each other than either is to the C.albicans protein is consistent with the idea that duplication and divergence of these proteins probably occurred in S.cerevisiae after the last common CandidaSaccharomyces ancestor, estimated to have existed ~300 million years ago (Pesole et al., 1995).

figure cde461f1
Fig. 1. Alignment of C.albicans Nrg1 (nrgc) with S.cerevisiae Nrg1 (nrg1s) and Nrg2 (nrg2s) (Park et al., 1999). Regions of identity are boxed. The zinc finger DNA-binding motif is shown in brackets and extends from amino acids 225 to 284 (of C.albicans ...
figure cde461f2
Fig. 2. Cell and colony morphology of nrg1, rfg1 and tup1 C.albicans mutants. Strains deleted for both copies of the indicated genes were streaked on YPD and SD plates, incubated for 4 days (SD) or 3 days (YPD) at 30°C and the resulting colonies ...

nrg1 mutants exhibit a high degree of filamentous growth

Deletion of both copies of NRG1 in C.albicans led to highly wrinkled, convoluted colonies of cells on YPD and on SD minimal media (Figure 2); this appearance is caused typically by a high proportion of filamentous cells (see below). These colonies also show unusual aerial projections. In contrast, the wild-type colonies were smooth under these same conditions. Microscopic examination of colonies of the nrg1 disruptant grown on SD and YPD showed them to contain a large number of filamentous cells, with the remainder appearing as normal blastospores (Figure 2). In contrast, wild-type cells grown on those same media for the same time periods were 100% blastospores. Growth on Spider medium, which partially induces filamentous growth in wild-type cells (50% of the cells were filamentous in this experiment), caused a complete conversion of the nrg1 strain to filamentous growth (data not shown). We conclude from these observations that Δnrg1 cells are predisposed to filamentous growth. Although the Δnrg1 mutant colonies appeared similar to Δtup1 colonies on YPD medium, the proportion of filamentous cells in the Δtup1 strain was higher, approaching 100%.

To test the idea that Nrg1 represses filamentous growth via the Tup1 pathway, we generated a Δnrg1/Δnrg1 Δtup1/Δtup1 double mutant. On YPD plates, this mutant appeared nearly identical to the Δtup1/Δtup1 mutant but possessed a more highly wrinkled crown and large numbers of tiny aerial projections not found on Δtup1/Δtup1 colonies (Figure 2). Based on these results, we believe that Nrg1 functions as a Tup1-dependent repressor, and that Nrg1 may account for a large part of the regulation of filamentous growth by Tup1. However, these results also suggest that Nrg1 may have a Tup1-independent role, perhaps as a gene activator in combination with a different protein. It is also possible that Tup1’s partner protein, Ssn6 (whose homolog exists in C.albicans, but has not been studied to date), may be able to provide some repression in a Tup1-independent manner. Both of these possibilities have precedents in S.cerevisiae (Treitel and Carlson, 1995).

The Δnrg1/Δnrg1 strain appears avirulent in a mouse model

As reviewed in the Introduction, most, if not all, Candida mutants that are altered in the blastospore to filament transition show defects in virulence as determined in the mouse tail-vein injection model (for reviews see Mitchell, 1998; Brown and Gow, 1999; Ernst, 2000). To determine whether the correlation holds for NRG1, we tested the effect of deleting NRG1 on virulence in an experiment that utilized 14 mice. Two mice were injected with saline, four with 1×106 cells of a wild-type strain (CAF2-1), four with 1×106 cells of a Δnrg1/Δnrg1 strain and four with 3.2×106 cells of a Δnrg1/Δnrg1 strain. By the end of the second day following injection, only the four mice injected with CAF2-1 had died. At the end of 30 days, all the mice injected with saline or with the Δnrg1/Δnrg1 strain were still alive and appeared healthy. These results indicate a strong requirement for NRG1 in a murine model of systemic candidiasis.

Nrg1 directs transcriptional repression of a subset of filament-specific genes

To investigate the nrg1 phenotype more fully, we studied the expression of three genes whose transcription is repressed in blastospores but activated during the transition to filamentous growth. ECE1 and HWP1 are both Tup1-controlled filament-specific genes (Birse et al., 1993; Staab et al., 1996; Braun and Johnson, 2000; Tsuchimori et al., 2000), and we found that both are constitutively expressed in the nrg1 mutant, regardless of growth conditions (Figure 3). The level of HWP1 and ECE1 expression is the same in the nrg1 tup1 double mutant as in either of the two single mutants, suggesting that Nrg1 directs transcriptional repression of these two genes via the Tup1 pathway. RBT1, another filament-specific gene also known to be under Tup1 control (Braun and Johnson, 2000; Braun et al., 2000), is only minimally, if at all, regulated by Nrg1. These results, as well as the colony morphologies (Figure 2), indicate that Nrg1 represses only a subset of the Tup1-repressed genes involved in filamentous growth, and suggest that filamentous growth in C.albicans is regulated by more than one DNA-binding protein that depends on Tup1 for repression activity. This situation is reminiscent of the multiple mechanisms of glucose repression in S.cerevisiae (Lutfiyya et al., 1998; Park et al., 1999).

figure cde461f3
Fig. 3. Comparison of filament-specific gene expression in nrg1- and tup1-deleted cells. RNA was prepared from a wild-type strain and strains bearing homozygous deletions of tup1, nrg1 and tup1 nrg1 grown under the indicated conditions for 2 h ...

Phenotypes of an nrg1 rfg1 double mutant

Two laboratories recently reported the identification of another Tup1-dependent DNA-binding protein from C.albicans, Rfg1, which is similar to the Rox1 protein of S.cerevisiae (Kadosh and Johnson, 2001; Khalaf and Zitomer, 2001). Like Nrg1, Rfg1 represses filamentation in C.albicans, although the effects of an RFG1 deletion are more subtle than those of an NRG1 deletion. Deletion of both RFG1 and NRG1 from C.albicans led to more wrinkled and ‘hairy’ colony morphologies on SD medium than those seen for either deletion alone, indicating a higher proportion of cells in the filamentous form (Figure 2). Although similar, the phenotypes of the double mutant and the tup1 mutant were not identical either in the morphology of colonies or in the complete conversion of cells from blastospore to filamentous forms. This result suggests that additional Tup1-dependent DNA-binding repressors may be involved in the regulation of filamentous growth and that Rfg1 and Nrg1 may have additional roles.

The Nrg1 transcript is down-regulated in response to serum at 37°C

How is Nrg1-dependent repression relieved during the blastospore–filament transition? Many mechanisms have been found to regulate Tup1-dependent transcriptional repressors in S.cerevisiae, including phosphorylation, subcellular localization and transcriptional control (Deckert et al., 1995a; De Vit et al., 1997; Treitel et al., 1998; for a review see Smith and Johnson, 2000). We studied the expression of NRG1 mRNA under various conditions that induce filamentous growth, and found that it was substantially reduced in response to growth in YPD medium with 10% fetal calf serum (FCS) at 37°C (Figure 4). The time course of this loss of NRG1 expression (half-life of ~1 h) roughly approximates the induction of filamentation, which begins to be visible within ~45 min under the conditions used. In contrast, we found that when cells were grown in Spider medium at 37°C, levels of NRG1 mRNA were not obviously reduced (Figure 4). Some caution is needed in interpreting this result, as Spider medium does not induce filamentous growth in 100% of the cells, and it is possible that NRG1 mRNA levels drop in those cells that do undergo the blastospore–filament transition. Down-regulation of the NRG1 transcript in response to serum at 37°C did not occur in strains deleted for EFG1, a major activator of filamentous growth. This observation suggests the possibility that one role of Efg1 during the induction of filamentous growth is the down-regulation of Nrg1. We note that Efg1 must have additional roles in controlling filamentous growth, as it can act independently of the Tup1 pathway (Braun and Johnson, 2000).

figure cde461f4
Fig. 4. Regulation of NRG1 mRNA abundance. Wild-type or homozygous mutant cells (as marked) were grown in the media indicated and probed for NRG1 mRNA via northern blot. Cells were grown in liquid for 1 h for YPD and YPD plus serum, and for 2 h for Spider. ...

To test directly the idea that down-regulation of NRG1 mRNA is involved in the induction of filament formation by serum at 37°C, we expressed NRG1 constitutively from the C.albicans ACT1 promoter. When transformed into a Δnrg1/Δnrg1 strain, ACT1::NRG1 fully suppressed the enhanced filamentous growth phenotype (data not shown). The ACT1::NRG1 construct (as well as a vector control) was next integrated in a wild-type strain and the corresponding transformants were tested for their ability to undergo the transition to filamentous growth in response to serum at 37°C. As shown in Figure 5 (rows 1 and 2), a wild-type strain transformed with vector only and grown on YPD medium with 10% serum at 37°C exhibited a wrinkled colony morphology and contained a significant proportion of hyphal cells when compared with the same strain grown at 30°C in the absence of serum. In contrast, however, the ACT1::NRG1 strain grew almost entirely in the blastospore form in both inducing (serum plus 37°C) and non-inducing (30°C) conditions (Figure 5, rows 3 and 4). These results demonstrate that unregulated (and possibly overexpressed) Nrg1 leads to a significant reduction in filamentous growth. Similar results were obtained when these strains were tested for induction by temperature alone and on Spider and cornmeal media (data not shown), indicating that the reduction of filamentous growth caused by unregulated Nrg1 is not restricted to the serum induction pathway. Northern analysis indicated that the ACT1::NRG1 Δnrg1/Δnrg1 strain produces roughly the same level of NRG1 mRNA as does the wild-type strain grown under non-filament-inducing conditions (data not shown).

figure cde461f5
Fig. 5. Unregulated expression of Nrg1 in wild-type and Tup1 deletion strains. A construct expressing Nrg1 constitutively from a C.albicans ACT1 promoter was integrated at the ACT1 locus in wild-type and Δtup1/Δtup1 strains. Strains ...

We next determined whether the reduction in filamentous growth caused by the unregulated NRG1 construct was dependent on Tup1. As shown in Figure 5 (rows 5–8), Δtup1/Δtup1 strains grown at 30°C in YPD or at 37°C in YPD plus 10% serum show constitutive hyphal growth regardless of whether they are expressing the ACT1 or ACT1::NRG1 constructs. These results indicate a clear requirement for Tup1 in the reduction of filamentous growth caused by unregulated Nrg1 and provide further support for the idea that in C.albicans, as in S.cerevisiae, Nrg1 carries out transcriptional repression via recruitment of the Tup1 co-repressor complex.


The switch between the blastospore (single-celled) form and the filamentous (many cells attached end-to-end) forms of the fungal pathogen C.albicans has been strongly implicated in its virulence. In this study, we identify a regulator of this transition, the DNA-binding repressor Nrg1. We provide evidence that Nrg1 acts through the general co-repressor Tup1 and we identify two filament-specific genes that are repressed by Nrg1 under non-inducing conditions. Finally, we provide evidence that the down-regulation of Nrg1 synthesis is responsible, at least in part, for the induction of filamentous growth in response to environmental stimuli.

Nrg1, a DNA-binding protein that represses C.albicans filamentous growth via the Tup1 pathway

Nrg1 and Rfg1 (another DNA-binding repressor protein) each have roles in regulating filamentous growth in C.albicans. They are similar in sequence to well-characterized DNA-binding proteins in S.cerevisiae that work through the general co-repressor Tup1. Here, we present four lines of evidence that directly implicate Tup1 in repression by Nrg1 in C.albicans: (i) nrg1 mutants are highly filamentous on non-inducing medium and appear similar (but not identical) to tup1 mutants; (ii) several filament-specific genes repressed by Nrg1 were identified and shown to be a subset of those genes repressed by Tup1; (iii) the transcripts of these genes are derepressed to the same extent in the nrg1 tup1 double mutant as in either of the two single mutants; and (iv) an ACT1::NRG1 construct inhibits C.albicans filamentous growth in a Tup1-dependent manner.

Comparisons between mutant strains (see Results) suggest that Nrg1 repression may account for a large part of the overall Tup1 phenotype. In contrast, rfg1 mutants are less filamentous than nrg1 strains and their colonies contain a smaller percentage of hyphal cells. While Rfg1 and Nrg1 may each regulate a subset of filament-specific genes, it seems likely that additional DNA-binding proteins (not yet identified) are also important for Tup1 repression of filamentous growth. Consistent with this idea, cells of the rfg1 nrg1 double mutant are not 100% hyphal, as are tup1 cells.

The observation that different Tup1-dependent DNA-binding proteins can regulate genes involved in the same process has precedents in S.cerevisiae. For example, Nrg1 of S.cerevisiae represses STA1, which encodes a starch-degrading enzyme, in the presence of glucose (Park et al., 1999). It has sequence similarity to several other S.cerevisiae proteins, including Mig1, Mig2 and Yer028, all of which are also glucose-dependent repressors that act by recruitment of Tup1 to promoters of target genes (Treitel and Carlson, 1995; Tzamarias and Struhl, 1995; Lutfiyya et al., 1998). Mig1 and Mig2 act on the same set of genes, although the way in which their repression is released is different. Mig1 is phosphorylated by Snf1 and excluded from the nucleus in the absence of glucose, while Mig2 is unaffected by Snf1, is constitutively nuclear and is turned into an active repressor in the presence of glucose by an unknown mechanism (Lutfiyya et al., 1998; Treitel et al., 1998; for a review see Johnston, 1999). Yer028 is known to be a glucose-dependent and Tup1-dependent repressor on the basis of LexA fusion protein experiments (Lutfiyya et al., 1998), but its target gene(s) and mechanism of glucose regulation are not known.

A model for induction of filamentous growth by serum at 37°C via the Nrg1–Tup1 pathway

The results presented in this paper provide insight into at least one mechanism by which growth in serum at 37°C induces filamentous growth in C.albicans. Under non-inducing conditions, Nrg1 functions as a strong Tup1-dependent repressor of a set of filament-specific, serum-inducible genes (Figure 3). In addition, the NRG1 transcript is itself regulated by serum at 37°C. Growth of blastospores in the presence of serum at 37°C rapidly induces filamentous growth, and the level of NRG1 mRNA decreases during this induction (Figure 4). When NRG1 is ectopically expressed from the actin promoter, it inhibits the blastospore–filament transition (Figure 5), consistent with the idea that Nrg1 expression normally is down-regulated as a part of the filamentous growth induction process.

These results suggest a model where, in the absence of inducing conditions, NRG1 is expressed and functions as a Tup1-dependent transcriptional repressor of a subset of filament-specific genes. In the presence of serum at 37°C, NRG1 transcript levels are down-regulated and, as a consequence, a set of filament-specific genes are derepressed and cells undergo the transition from blastospores to filaments (Figure 6). At this point, we do not know whether transcription of the NRG1 gene is repressed or whether NRG1 mRNA is degraded during induction of filamentous growth, or both. Given that Δnrg1 mutants are only partially filamentous, it seems likely that NRG1 down-regulation is only one of several mechanisms by which filamentous growth is induced.

figure cde461f6
Fig. 6. A model for induction of hyphal-specific genes by Nrg1 in the presence of serum at 37°C. In the absence of serum and at 37°C, Nrg1 is expressed and binds to the promoters of a subset of hyphal-specific genes. Nrg1 functions as ...

Regulation of filamentous growth in C.albicans by condition-specific DNA-binding proteins

Candida albicans undergoes the transition from blastospore to filament forms in response to a wide variety of environmental conditions including neutral pH, serum, high temperature (37°C), high CO2/O2 ratio and starvation (Gow and Gooday, 1984; Odds, 1985, 1988). The finding that Nrg1, a strong transcriptional repressor of filament-specific genes, is specifically down-regulated in response to growth in serum at 37°C supports recent observations that different DNA-binding proteins regulate transcription of hyphal genes in response to different environmental conditions. For example, the Prr2 regulatory protein induces alkaline-specific transcripts and represses acid-specific transcripts at neutral pH (Ramon et al., 1999; Davis et al., 2000). Efg1, an APSES domain basic helix–loop–helix (bHLH) DNA-binding protein, has been shown to regulate induction of C.albicans filamentous growth in response to serum, N-acetyl glucosamine (GlcNAc) and starvation; Efg1 is believed to be the downstream target of a cAMP/PKA signaling pathway (Lo et al., 1997; Stoldt et al., 1997; Ernst, 2000). Czf1, a zinc finger DNA-binding protein, is important for filamentous growth induction specifically in the presence of microaerophilic, or embedded, conditions (Brown et al., 1999).

These findings all suggest that induction of filamentous growth in C.albicans is a complex process involving multiple DNA-binding proteins that respond to a wide variety of environmental conditions. Moreover, it is becoming increasingly apparent that each different DNA-binding protein regulates a different subset of filament-specific genes (Murad et al., 2001). This observation may, in part, explain how a wide variety of filament types can be generated by C.albicans in response to different environmental conditions.

Materials and methods

Strains and plasmids

Deletion of NRG1 was performed by the URA blaster technique described previously (Fonzi and Irwin, 1993; Braun et al., 2000), starting from the strain CAI4 (Fonzi and Irwin, 1993), creating heterozygous strain BCa23-1 and homozygous strain BCa23-3. Short DNA segments flanking the NRG1 open reading frame (ORF) were synthesized using the following primers: 5′-GTATAAAGGCATGCAGATTTCCCTCT-3′ (SphI), 5′-TGATTGTTGGATCCTTAATGAAACT-3′ (BamHI), 5′-CAAGAGCCTAGTATGCATGTGGTCAA-3′ (NsiI) and 5′-TTTTTGGGGTACCCAAGAAATAATTGC-3′ (Asp718), and placed on either side of a URA3 marker gene from pBB510 (Braun and Johnson, 2000) to create the disruption constructs pBB571A and pBB571B. The transforming DNA fragment was released by cleaving these constructs with SphI and Asp718, followed by transformation into CAI4 or heterozygous BCa23-1 cells, respectively. PCR was used to verify each junction of the new locus and the removal of the native locus. These disruptions removed the entire coding region of NRG1, from 10 bp before the ATG, to the T of the TAG stop codon. Disruption of RFG1 has been described previously (Kadosh and Johnson, 2001). Sequence data for C.albicans were obtained from the Stanford DNA Sequencing and Technology Center website at http://www-sequence.stanford.edu/group/candida.

The ACT1::NRG1 expression construct was generated as follows: a PCR fragment containing the entire NRG1 ORF (flanked by an upstream XhoI site and a downstream BamHI site) was cloned into the pDK1 ACT1 expression vector cut with XhoI and BamHI (pDK1 was generated by cloning a 3.4 kb SmaI–AvrII restriction fragment containing rahB–URA3–rahB repeats into ACT1 expression vector pAU34; Uhl and Johnson, 2001). The resulting construct (p576) was then cut in the ACT1 promoter DNA with BsrGI to direct integration to the native ACT1 locus. Positive transformants were verified by PCR.

Growth conditions and RNA preparation

Cells were grown routinely on YPD and SD minimal selective medium at 30°C (Guthrie and Fink, 1991). Cells were tested for filamentous growth on YPD, Spider agar, liquid Spider medium and liquid YPD + 10% FCS at both 30 and 37°C, as described previously (Braun and Johnson, 2000). The wild-type strain used was CAF2-1 (Fonzi and Irwin, 1993), which carries one copy of URA3, as do all of the disruptant strains.

For RNA analysis, cells were grown overnight to OD600 ~4.0, rediluted to OD600 ~0.5 in either inducing or non-inducing medium and grown at either 30 or 37°C for the times indicated. RNA was prepared by the hot phenol method (Guthrie and Fink, 1991; Ausubel et al., 1992) and 5 µg were electrophoresed on formaldehyde gels before capillary transfer to Gene Screen nylon membranes. Transfer and loading were monitored by ethidium bromide staining. Northern blots were hybridized as described previously (Church and Gilbert, 1984) at 67–70°C. The NRG1 hybridization probe was made from a PCR fragment covering the entire gene (5′-CAATTTGGATCCCATCTATACTAGGC-3′ to 5′-GGCCTC GAGCATTATGCTTTATCAACAATCA-3′). The RBT1, HWP1 and ECE1 probes, comprising unique portions of their ORFs, have been described previously (Braun and Johnson, 2000). Quantitation of NRG1 mRNA was performed by phosphoimager.

Virulence experiments

General procedures were as described in Braun et al. (2000). Δnrg1/Δnrg1 and wild-type cells were grown overnight to ~OD600 1.0 in YPD (wild-type cells were back-diluted once from OD600 1.0 to OD600 0.2 to allow the slower growing nrg1 cells to catch up). At this point, the larger (uninjectable) clumps were allowed to settle out briefly and the suspended cells were collected, rinsed twice in phosphate-buffered saline and counted on the hemocytometer. This sample consisted of small clumps of 1–50 pseudohyphal cells. Due to the filamentous nature of the Δnrg1/Δnrg1 cells, their OD600 and cell counts were less reliable than those of wild-type cells. For this reason, we performed the nrg1 injections with both 1 × 106 and 3.2 × 106 cells, for comparison with wild-type cells at 1 × 106 cells per mouse. Injections were performed on female Balb/c mice (8–10 weeks old; Charles River Co., Cambridge, MA) and survival was monitored over a period of 30 days.


We thank M.Miller for technical assistance, A.Uhl for constructs, P.O’Farrell for use of his laboratory’s differential interference microscope, and members of the Johnson laboratory for fruitful discussions during the course of the experiments. We also thank A.J.P.Brown and colleagues for communication of results prior to publication. Sequence data for C.albicans were obtained from the Stanford DNA Sequencing and Technology Center website at http://www-sequence.stanford.edu/group/candida and we are grateful for the support this project has provided to the Candida community. Sequencing of C.albicans was accomplished with the support of the NIDR and the Burroughs Wellcome Fund. The work reported in this paper was supported by NIH grant GM 37049 to A.D.J. and by a fellowship from the Cancer Research Fund of the Damon Runyan–Walter Winchell Foundation, DRG-1512, to D.K.


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