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Environ Health Perspect. 2004 Apr; 112(5): 626–630.
PMCID: PMC1241932
Research Article

Molecular evidence of an interaction between prenatal environmental exposures and birth outcomes in a multiethnic population.


Inner-city, minority populations are high-risk groups for adverse birth outcomes and also are more likely to be exposed to environmental contaminants, including environmental tobacco smoke (ETS), benzo[a]pyrene (BaP), and other polycyclic aromatic hydrocarbons (PAHs) found in urban air. In a sample of nonsmoking African-American and Dominican women, we evaluated the effects on birth outcomes of prenatal exposure to ETS, using questionnaire data and plasma cotinine as a biomarker of exposure, and environmental PAHs using BaP-DNA adducts as a molecular dosimeter. We previously reported that among African Americans, high prenatal exposure to PAHs estimated by prenatal personal air monitoring was associated with lower birth weight (p = 0.003) and smaller head circumference (p = 0.01) after adjusting for potential confounders. In the present analysis, self-reported ETS was associated with decreased head circumference (p = 0.04). BaP-DNA adducts were not correlated with ETS or dietary PAHs. There was no main effect of BaP-DNA adducts on birth outcomes. However, there was a significant interaction between the two pollutants such that the combined exposure to high ETS and high adducts had a significant multiplicative effect on birth weight (p = 0.04) and head circumference (p = 0.01) after adjusting for ethnicity, sex of newborns, maternal body mass index, dietary PAHs, and gestational age. This study provides evidence that combined exposure to environmental pollutants at levels currently encountered in New York City adversely affects fetal development.

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Selected References

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  • Alexandrov K, Rojas M, Geneste O, Castegnaro M, Camus AM, Petruzzelli S, Giuntini C, Bartsch H. An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung: comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity. Cancer Res. 1992 Nov 15;52(22):6248–6253. [PubMed]
  • Barbieri O, Ognio E, Rossi O, Astigiano S, Rossi L. Embryotoxicity of benzo(a)pyrene and some of its synthetic derivatives in Swiss mice. Cancer Res. 1986 Jan;46(1):94–98. [PubMed]
  • Bartsch H. DNA adducts in human carcinogenesis: etiological relevance and structure-activity relationship. Mutat Res. 1996 Jun;340(2-3):67–79. [PubMed]
  • Bernert JT, Jr, McGuffey JE, Morrison MA, Pirkle JL. Comparison of serum and salivary cotinine measurements by a sensitive high-performance liquid chromatography-tandem mass spectrometry method as an indicator of exposure to tobacco smoke among smokers and nonsmokers. J Anal Toxicol. 2000 Jul-Aug;24(5):333–339. [PubMed]
  • Bernert JT, Jr, Turner WE, Pirkle JL, Sosnoff CS, Akins JR, Waldrep MK, Ann Q, Covey TR, Whitfield WE, Gunter EW, et al. Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry. Clin Chem. 1997 Dec;43(12):2281–2291. [PubMed]
  • Bobak M. Outdoor air pollution, low birth weight, and prematurity. Environ Health Perspect. 2000 Feb;108(2):173–176. [PMC free article] [PubMed]
  • Boström Carl-Elis, Gerde Per, Hanberg Annika, Jernström Bengt, Johansson Christer, Kyrklund Titus, Rannug Agneta, Törnqvist Margareta, Victorin Katarina, Westerholm Roger. Cancer risk assessment, indicators, and guidelines for polycyclic aromatic hydrocarbons in the ambient air. Environ Health Perspect. 2002 Jun;110 (Suppl 3):451–488. [PMC free article] [PubMed]
  • Bui QQ, Tran MB, West WL. A comparative study of the reproductive effects of methadone and benzo[a]pyrene in the pregnant and pseudopregnant rat. Toxicology. 1986 Dec 15;42(2-3):195–204. [PubMed]
  • Chaikind S, Corman H. The impact of low birthweight on special education costs. J Health Econ. 1991 Oct;10(3):291–311. [PubMed]
  • Chen L, Omaye ST. Air pollution and health effects in northern Nevada. Rev Environ Health. 2001 Apr-Jun;16(2):133–149. [PubMed]
  • Chen LH, Petitti DB. Case-control study of passive smoking and the risk of small-for-gestational-age at term. Am J Epidemiol. 1995 Jul 15;142(2):158–165. [PubMed]
  • Davis DL, Bradlow HL, Wolff M, Woodruff T, Hoel DG, Anton-Culver H. Medical hypothesis: xenoestrogens as preventable causes of breast cancer. Environ Health Perspect. 1993 Oct;101(5):372–377. [PMC free article] [PubMed]
  • Dejmek J, Solanský I, Benes I, Lenícek J, Srám RJ. The impact of polycyclic aromatic hydrocarbons and fine particles on pregnancy outcome. Environ Health Perspect. 2000 Dec;108(12):1159–1164. [PMC free article] [PubMed]
  • Desch LW, Anderson SK, Snow JH. Relationship of head circumference to measures of school performance. Clin Pediatr (Phila) 1990 Jul;29(7):389–392. [PubMed]
  • Eskenazi B, Prehn AW, Christianson RE. Passive and active maternal smoking as measured by serum cotinine: the effect on birthweight. Am J Public Health. 1995 Mar;85(3):395–398. [PMC free article] [PubMed]
  • Etzel RA. Active and passive smoking: hazards for children. Cent Eur J Public Health. 1997 Jun;5(2):54–56. [PubMed]
  • Faustman EM, Silbernagel SM, Fenske RA, Burbacher TM, Ponce RA. Mechanisms underlying Children's susceptibility to environmental toxicants. Environ Health Perspect. 2000 Mar;108 (Suppl 1):13–21. [PMC free article] [PubMed]
  • Ha EH, Hong YC, Lee BE, Woo BH, Schwartz J, Christiani DC. Is air pollution a risk factor for low birth weight in Seoul? Epidemiology. 2001 Nov;12(6):643–648. [PubMed]
  • Janerich DT, Thompson WD, Varela LR, Greenwald P, Chorost S, Tucci C, Zaman MB, Melamed MR, Kiely M, McKneally MF. Lung cancer and exposure to tobacco smoke in the household. N Engl J Med. 1990 Sep 6;323(10):632–636. [PubMed]
  • Landrigan PJ, Claudio L, Markowitz SB, Berkowitz GS, Brenner BL, Romero H, Wetmur JG, Matte TD, Gore AC, Godbold JH, et al. Pesticides and inner-city children: exposures, risks, and prevention. Environ Health Perspect. 1999 Jun;107 (Suppl 3):431–437. [PMC free article] [PubMed]
  • Legraverend C, Guenthner TM, Nebert DW. Importance of the route of administration for genetic differences in benzo[a]pyrene-induced in utero toxicity and teratogenicity. Teratology. 1984 Feb;29(1):35–47. [PubMed]
  • Leikauf GD, Kline S, Albert RE, Baxter CS, Bernstein DI, Bernstein J, Buncher CR. Evaluation of a possible association of urban air toxics and asthma. Environ Health Perspect. 1995 Sep;103 (Suppl 6):253–271. [PMC free article] [PubMed]
  • Manchester DK, Gordon SK, Golas CL, Roberts EA, Okey AB. Ah receptor in human placenta: stabilization by molybdate and characterization of binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin, 3-methylcholanthrene, and benzo(a)pyrene. Cancer Res. 1987 Sep 15;47(18):4861–4868. [PubMed]
  • Martinez FD, Wright AL, Taussig LM. The effect of paternal smoking on the birthweight of newborns whose mothers did not smoke. Group Health Medical Associates. Am J Public Health. 1994 Sep;84(9):1489–1491. [PMC free article] [PubMed]
  • Matte TD, Bresnahan M, Begg MD, Susser E. Influence of variation in birth weight within normal range and within sibships on IQ at age 7 years: cohort study. BMJ. 2001 Aug 11;323(7308):310–314. [PMC free article] [PubMed]
  • Metzger R, Delgado JL, Herrell R. Environmental health and Hispanic children. Environ Health Perspect. 1995 Sep;103 (Suppl 6):25–32. [PMC free article] [PubMed]
  • Meyn MS. Ataxia-telangiectasia and cellular responses to DNA damage. Cancer Res. 1995 Dec 15;55(24):5991–6001. [PubMed]
  • Nicol CJ, Harrison ML, Laposa RR, Gimelshtein IL, Wells PG. A teratologic suppressor role for p53 in benzo[a]pyrene-treated transgenic p53-deficient mice. Nat Genet. 1995 Jun;10(2):181–187. [PubMed]
  • Perera Frederica P, Illman Susan M, Kinney Patrick L, Whyatt Robin M, Kelvin Elizabeth A, Shepard Peggy, Evans David, Fullilove Mindy, Ford Jean, Miller Rachel L, et al. The challenge of preventing environmentally related disease in young children: community-based research in New York City. Environ Health Perspect. 2002 Feb;110(2):197–204. [PMC free article] [PubMed]
  • Perera FP, Jedrychowski W, Rauh V, Whyatt RM. Molecular epidemiologic research on the effects of environmental pollutants on the fetus. Environ Health Perspect. 1999 Jun;107 (Suppl 3):451–460. [PMC free article] [PubMed]
  • Perera Frederica P, Rauh Virginia, Tsai Wei-Yann, Kinney Patrick, Camann David, Barr Dana, Bernert Tom, Garfinkel Robin, Tu Yi-Hsuan, Diaz Diurka, et al. Effects of transplacental exposure to environmental pollutants on birth outcomes in a multiethnic population. Environ Health Perspect. 2003 Feb;111(2):201–205. [PMC free article] [PubMed]
  • Perera FP, Whyatt RM, Jedrychowski W, Rauh V, Manchester D, Santella RM, Ottman R. Recent developments in molecular epidemiology: A study of the effects of environmental polycyclic aromatic hydrocarbons on birth outcomes in Poland. Am J Epidemiol. 1998 Feb 1;147(3):309–314. [PubMed]
  • Pirkle JL, Flegal KM, Bernert JT, Brody DJ, Etzel RA, Maurer KR. Exposure of the US population to environmental tobacco smoke: the Third National Health and Nutrition Examination Survey, 1988 to 1991. JAMA. 1996 Apr 24;275(16):1233–1240. [PubMed]
  • Schuster-Kolbe J, Ludwig H. Rauchen und Krebsrisiko. Wien Med Wochenschr. 1994;144(22-23):540–544. [PubMed]
  • Sexton M, Fox NL, Hebel JR. Prenatal exposure to tobacco: II. Effects on cognitive functioning at age three. Int J Epidemiol. 1990 Mar;19(1):72–77. [PubMed]
  • Wagenknecht LE, Manolio TA, Sidney S, Burke GL, Haley NJ. Environmental tobacco smoke exposure as determined by cotinine in black and white young adults: the CARDIA Study. Environ Res. 1993 Oct;63(1):39–46. [PubMed]
  • Whyatt Robin M, Barr Dana B, Camann David E, Kinney Patrick L, Barr John R, Andrews Howard F, Hoepner Lori A, Garfinkel Robin, Hazi Yair, Reyes Andria, et al. Contemporary-use pesticides in personal air samples during pregnancy and blood samples at delivery among urban minority mothers and newborns. Environ Health Perspect. 2003 May;111(5):749–756. [PMC free article] [PubMed]
  • Whyatt Robin M, Camann David E, Kinney Patrick L, Reyes Andria, Ramirez Judy, Dietrich Jessica, Diaz Diurka, Holmes Darrell, Perera Frederica P. Residential pesticide use during pregnancy among a cohort of urban minority women. Environ Health Perspect. 2002 May;110(5):507–514. [PMC free article] [PubMed]
  • Whyatt RM, Perera FP. Application of biologic markers to studies of environmental risks in children and the developing fetus. Environ Health Perspect. 1995 Sep;103 (Suppl 6):105–110. [PMC free article] [PubMed]
  • Wood KA, Youle RJ. The role of free radicals and p53 in neuron apoptosis in vivo. J Neurosci. 1995 Aug;15(8):5851–5857. [PubMed]

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