• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biophysjLink to Publisher's site
Biophys J. Dec 1995; 69(6): 2364–2368.
PMCID: PMC1236473

Stationarity of sodium channel gating kinetics in excised patches from neuroblastoma N1E 115.

Abstract

Na channel gating parameters in a number of preparations are translated along the voltage axis in excised patches compared to cell attached or whole cell recording. The aim of this study is to determine whether these changes in gating behavior continue over an extended period or, rather, develop rapidly on excision with stationary kinetics thereafter. Average currents were constructed from single-channel records from neuroblastoma N1E 115 at various times after excision, excluding the first 5 min, in eight inside-out excised patches. Single exponentials were fitted to the current decay of the average records, and the mean time constant for each patch was determined. Values were plotted as the percentage difference from these means for each patch against time from excision. Collected results show no obvious trend in values from 5 min to 2 h. Kinetics are stationary, and shifts in Na channel gating parameters along the voltage axis seen in excised as compared to whole cell configuration in neuroblastoma must be complete by the first few minutes after excision. Raising the internal Na concentration reduced the single channel current amplitude, confirming that these are Na channels.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (694K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aldrich RW, Corey DP, Stevens CF. A reinterpretation of mammalian sodium channel gating based on single channel recording. Nature. 1983 Dec 1;306(5942):436–441. [PubMed]
  • Aldrich RW, Stevens CF. Voltage-dependent gating of single sodium channels from mammalian neuroblastoma cells. J Neurosci. 1987 Feb;7(2):418–431. [PubMed]
  • Cachelin AB, De Peyer JE, Kokubun S, Reuter H. Sodium channels in cultured cardiac cells. J Physiol. 1983 Jul;340:389–401. [PMC free article] [PubMed]
  • Cannell MB, Lederer WJ. A novel experimental chamber for single-cell voltage-clamp and patch-clamp applications with low electrical noise and excellent temperature and flow control. Pflugers Arch. 1986 May;406(5):536–539. [PubMed]
  • Fenwick EM, Marty A, Neher E. Sodium and calcium channels in bovine chromaffin cells. J Physiol. 1982 Oct;331:599–635. [PMC free article] [PubMed]
  • Fernandez JM, Fox AP, Krasne S. Membrane patches and whole-cell membranes: a comparison of electrical properties in rat clonal pituitary (GH3) cells. J Physiol. 1984 Nov;356:565–585. [PMC free article] [PubMed]
  • Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 1981 Aug;391(2):85–100. [PubMed]
  • Kohlhardt M, Fröbe U, Herzig JW. Properties of normal and non-inactivating single cardiac Na+ channels. Proc R Soc Lond B Biol Sci. 1987 Oct 22;232(1266):71–93. [PubMed]
  • Kunze DL, Lacerda AE, Wilson DL, Brown AM. Cardiac Na currents and the inactivating, reopening, and waiting properties of single cardiac Na channels. J Gen Physiol. 1985 Nov;86(5):691–719. [PMC free article] [PubMed]
  • Moolenaar WH, Spector I. Membrane currents examined under voltage clamp in cultured neuroblastoma cells. Science. 1977 Apr 15;196(4287):331–333. [PubMed]
  • Nagy K, Kiss T, Hof D. Single Na channels in mouse neuroblastoma cell membrane. Indications for two open states. Pflugers Arch. 1983 Dec;399(4):302–308. [PubMed]
  • Quandt FN. Burst kinetics of sodium channels which lack fast inactivation in mouse neuroblastoma cells. J Physiol. 1987 Nov;392:563–585. [PMC free article] [PubMed]
  • Shenkel S, Sigworth FJ. Patch recordings from the electrocytes of Electrophorus electricus. Na currents and PNa/PK variability. J Gen Physiol. 1991 May;97(5):1013–1041. [PMC free article] [PubMed]
  • Vandenberg CA, Bezanilla F. A sodium channel gating model based on single channel, macroscopic ionic, and gating currents in the squid giant axon. Biophys J. 1991 Dec;60(6):1511–1533. [PMC free article] [PubMed]
  • Vandenberg CA, Horn R. Inactivation viewed through single sodium channels. J Gen Physiol. 1984 Oct;84(4):535–564. [PMC free article] [PubMed]
  • Yamamoto D, Yeh JZ, Narahashi T. Voltage-dependent calcium block of normal and tetramethrin-modified single sodium channels. Biophys J. 1984 Jan;45(1):337–344. [PMC free article] [PubMed]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

Formats:

Related citations in PubMed

See reviews...See all...

Links

  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...