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Can J Comp Med. 1983 July; 47(3): 328–331. | PMCID: PMC1235947 |
Syncytium-induction inhibition test with complement for detection of antibodies against bovine leukemia virus. Y Kono, W Irishio, and H Sentsui Abstract A modified syncytium-induction inhibition test which is more sensitive than the immunodiffusion test, was developed using rabbit complement. In this test, fetal lamb kidney cells continuously infected with bovine leukemia virus were used as effector cells, and the CC81 cat cells transformed with murine sarcoma virus, were used as indicator cells. The syncytium-induction inhibition effect of anti-bovine leukemia virus serum was enhanced significantly by the addition of rabbit complement. The syncytium-induction inhibition titers had a statistically significant correlation with the immunodiffusion titers and were four to 64 times higher than immunodiffusion titers. In 12 experimentally infected cattle, the syncytium-induction inhibition test detected the antibodies earlier than the immunodiffusion test and continuously detected them when immunodiffusion antibody changed to negative. In the 81 sera from naturally infected herds, 35 (43.2%) were positive by the immunodiffusion test and 55 (67.9%) by the syncytium-induction inhibition test. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (625K), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. These references are in PubMed. This may not be the complete list of references from this article. - Devare SG, Chander S, Samagh BS, Stephenson JR. Evaluation of radioimmunoprecipitation for the detection of bovine leukemia virus infection in domestic cattle. J Immunol. 1977 Jul;119(1):277–282. [PubMed]
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