• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biophysjLink to Publisher's site
Biophys J. May 1996; 70(5): 2437–2441.
PMCID: PMC1225221

Specific antigen/antibody interactions measured by force microscopy.

Abstract

Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to their antigens when probed with an AFM in the force mode. We could measure the rupture force between individual antibody-antigen complexes. The potential and limitations of this new approach for the measurement of individual antigen/antibody interactions and some possible applications are discussed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Binnig G, Quate CF, Gerber C. Atomic force microscope. Phys Rev Lett. 1986 Mar 3;56(9):930–933. [PubMed]
  • Brada D, Roth J. "Golden blot"--detection of polyclonal and monoclonal antibodies bound to antigens on nitrocellulose by protein A-gold complexes. Anal Biochem. 1984 Oct;142(1):79–83. [PubMed]
  • Chaiken I, Rosé S, Karlsson R. Analysis of macromolecular interactions using immobilized ligands. Anal Biochem. 1992 Mar;201(2):197–210. [PubMed]
  • Dammer U, Popescu O, Wagner P, Anselmetti D, Güntherodt HJ, Misevic GN. Binding strength between cell adhesion proteoglycans measured by atomic force microscopy. Science. 1995 Feb 24;267(5201):1173–1175. [PubMed]
  • Davies DR, Padlan EA, Sheriff S. Antibody-antigen complexes. Annu Rev Biochem. 1990;59:439–473. [PubMed]
  • Florin EL, Moy VT, Gaub HE. Adhesion forces between individual ligand-receptor pairs. Science. 1994 Apr 15;264(5157):415–417. [PubMed]
  • Goldblatt D, van Etten L, van Milligen FJ, Aalberse RC, Turner MW. The role of pH in modified ELISA procedures used for the estimation of functional antibody affinity. J Immunol Methods. 1993 Dec 3;166(2):281–285. [PubMed]
  • Hansma HG, Hoh JH. Biomolecular imaging with the atomic force microscope. Annu Rev Biophys Biomol Struct. 1994;23:115–139. [PubMed]
  • Ill CR, Keivens VM, Hale JE, Nakamura KK, Jue RA, Cheng S, Melcher ED, Drake B, Smith MC. A COOH-terminal peptide confers regiospecific orientation and facilitates atomic force microscopy of an IgG1. Biophys J. 1993 Mar;64(3):919–924. [PMC free article] [PubMed]
  • Kelley RF, O'Connell MP. Thermodynamic analysis of an antibody functional epitope. Biochemistry. 1993 Jul 13;32(27):6828–6835. [PubMed]
  • Lee GU, Chrisey LA, Colton RJ. Direct measurement of the forces between complementary strands of DNA. Science. 1994 Nov 4;266(5186):771–773. [PubMed]
  • Morris VJ. Biological applications of scanning probe microscopies. Prog Biophys Mol Biol. 1994;61(2):131–185. [PubMed]
  • Moy VT, Florin EL, Gaub HE. Intermolecular forces and energies between ligands and receptors. Science. 1994 Oct 14;266(5183):257–259. [PubMed]
  • Radmacher M, Cleveland JP, Fritz M, Hansma HG, Hansma PK. Mapping interaction forces with the atomic force microscope. Biophys J. 1994 Jun;66(6):2159–2165. [PMC free article] [PubMed]
  • van Oss CJ. Aspecific and specific intermolecular interactions in aqueous media. J Mol Recognit. 1990 Jun;3(3):128–136. [PubMed]
  • Wagner P, Kernen P, Hegner M, Ungewickell E, Semenza G. Covalent anchoring of proteins onto gold-directed NHS-terminated self-assembled monolayers in aqueous buffers: SFM images of clathrin cages and triskelia. FEBS Lett. 1994 Dec 19;356(2-3):267–271. [PubMed]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links