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Biochem J. Dec 1, 2003; 376(Pt 2): 433–440.
PMCID: PMC1223786

Structural and biochemical characterization of a new type of lectin isolated from carp eggs.


A previously unidentified glycoprotein present in the eggs of the carp ( Cyprinus carpio ) was isolated and structurally characterized. The protein binds to a Sepharose 4B matrix and can be eluted with 0.4 M N -acetylglucosamine. The protein has an apparent molecular mass of 26686.3 Da. On the basis of gel-filtration chromatography, the protein appears to be present in solution as a monomer. The sequence of its 238 amino acids, the position of its four disulphide bridges and the composition of its single N-linked carbohydrate chain were determined. The lectin shows a very low agglutinating activity for human A-type erythrocytes and interacts with both Gram-positive and -negative bacteria. These latter interactions are inhibited by N -acetylglucosamine. A database search shows that its amino acid sequence is similar to that of the members of an invertebrate lectin family that includes tachylectin-1. Tachylectin-1 is present in the amoebocytes of the horseshoe crab, Tachypleus tridentatus, and plays a role in the innate defence system of this species. Homologous genes are also present in other fish, having 85% identity with a gene expressed in the oocytes of the crucian carp ( Carassius auratus gibelio ) and 78% identity with a gene in the cDNA library of the zebrafish ( Danio rerio ).

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Selected References

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  • Lis Halina, Sharon Nathan. Lectins: Carbohydrate-Specific Proteins That Mediate Cellular Recognition. Chem Rev. 1998 Apr 2;98(2):637–674. [PubMed]
  • Sacchettini JC, Baum LG, Brewer CF. Multivalent protein-carbohydrate interactions. A new paradigm for supermolecular assembly and signal transduction. Biochemistry. 2001 Mar 13;40(10):3009–3015. [PubMed]
  • Dodd RB, Drickamer K. Lectin-like proteins in model organisms: implications for evolution of carbohydrate-binding activity. Glycobiology. 2001 May;11(5):71R–79R. [PubMed]
  • Rudd PM, Elliott T, Cresswell P, Wilson IA, Dwek RA. Glycosylation and the immune system. Science. 2001 Mar 23;291(5512):2370–2376. [PubMed]
  • Hoffmann JA, Kafatos FC, Janeway CA, Ezekowitz RA. Phylogenetic perspectives in innate immunity. Science. 1999 May 21;284(5418):1313–1318. [PubMed]
  • Iwanaga S, Kawabata S, Muta T. New types of clotting factors and defense molecules found in horseshoe crab hemolymph: their structures and functions. J Biochem. 1998 Jan;123(1):1–15. [PubMed]
  • Kawabata S, Iwanaga S. Role of lectins in the innate immunity of horseshoe crab. Dev Comp Immunol. 1999 Jun-Jul;23(4-5):391–400. [PubMed]
  • Iwanaga Sadaaki. The molecular basis of innate immunity in the horseshoe crab. Curr Opin Immunol. 2002 Feb;14(1):87–95. [PubMed]
  • Saito T, Kawabata S, Hirata M, Iwanaga S. A novel type of limulus lectin-L6. Purification, primary structure, and antibacterial activity. J Biol Chem. 1995 Jun 16;270(24):14493–14499. [PubMed]
  • Saito T, Hatada M, Iwanaga S, Kawabata S. A newly identified horseshoe crab lectin with binding specificity to O-antigen of bacterial lipopolysaccharides. J Biol Chem. 1997 Dec 5;272(49):30703–30708. [PubMed]
  • Nagai T, Kawabata S, Shishikura F, Sugita H. Purification, characterization, and amino acid sequence of an embryonic lectin in perivitelline fluid of the horseshoe crab. J Biol Chem. 1999 Dec 31;274(53):37673–37678. [PubMed]
  • Chiou ST, Chen YW, Chen SC, Chao CF, Liu TY. Isolation and characterization of proteins that bind to galactose, lipopolysaccharide of Escherichia coli, and protein A of Staphylococcus aureus from the hemolymph of Tachypleus tridentatus. J Biol Chem. 2000 Jan 21;275(3):1630–1634. [PubMed]
  • Chen SC, Yen CH, Yeh MS, Huang CJ, Liu TY. Biochemical properties and cDNa cloning of two new lectins from the plasma of Tachypleus tridentatus: Tachypleus plasma lectin 1 and 2+. J Biol Chem. 2001 Mar 30;276(13):9631–9639. [PubMed]
  • Magor BG, Magor KE. Evolution of effectors and receptors of innate immunity. Dev Comp Immunol. 2001 Oct-Dec;25(8-9):651–682. [PubMed]
  • Ellis AE. Innate host defense mechanisms of fish against viruses and bacteria. Dev Comp Immunol. 2001 Oct-Dec;25(8-9):827–839. [PubMed]
  • Jones SR. The occurrence and mechanisms of innate immunity against parasites in fish. Dev Comp Immunol. 2001 Oct-Dec;25(8-9):841–852. [PubMed]
  • Tateno H, Saneyoshi A, Ogawa T, Muramoto K, Kamiya H, Saneyoshi M. Isolation and characterization of rhamnose-binding lectins from eggs of steelhead trout (Oncorhynchus mykiss) homologous to low density lipoprotein receptor superfamily. J Biol Chem. 1998 Jul 24;273(30):19190–19197. [PubMed]
  • Hosono M, Ishikawa K, Mineki R, Murayama K, Numata C, Ogawa Y, Takayanagi Y, Nitta K. Tandem repeat structure of rhamnose-binding lectin from catfish (Silurus asotus) eggs. Biochim Biophys Acta. 1999 Nov 16;1472(3):668–675. [PubMed]
  • Kubo T, Kawasaki K, Natori S. Transient appearance and localization of a 26-kDa lectin, a novel member of the Periplaneta lectin family, in regenerating cockroach leg. Dev Biol. 1993 Apr;156(2):381–390. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Huh CG, Aldrich J, Mottahedeh J, Kwon H, Johnson C, Marsh R. Cloning and characterization of Physarum polycephalum tectonins. Homologues of Limulus lectin L-6. J Biol Chem. 1998 Mar 13;273(11):6565–6574. [PubMed]
  • Fülöp V, Jones DT. Beta propellers: structural rigidity and functional diversity. Curr Opin Struct Biol. 1999 Dec;9(6):715–721. [PubMed]
  • Xie J, Wen JJ, Chen B, Gui JF. Differential gene expression in fully-grown oocytes between gynogenetic and gonochoristic crucian carps. Gene. 2001 Jun 13;271(1):109–116. [PubMed]

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