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Biochem J. Sep 1, 2003; 374(Pt 2): 497–503.
PMCID: PMC1223617

Association of human kinesin superfamily protein member 4 with BRCA2-associated factor 35.


A large portion of human kinesin superfamily protein member 4 (KIF4) is associated with the nuclear matrix during the interphase, while a small portion is found in the cytoplasm. During mitosis, it is associated with chromosomes throughout the entire process. In the present study, we identified a protein that interacts with KIF4 using a yeast two-hybrid system, co-immunoprecipitation and co-fractionation. This protein is BRCA2-associated factor 35 (BRAF35) containing a non-specific DNA binding high-mobility-group domain and a kinesin-like coiled-coil domain. It appeared that the interaction between the two proteins occurs through their respective alpha-helical coiled-coil domains. The co-fractionation experiment revealed that KIF4 and BRAF35 were present in a complex of approx. 540 kDa. The composition and biological significance of this complex should be studied further.

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Selected References

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  • Rieder CL, Salmon ED. The vertebrate cell kinetochore and its roles during mitosis. Trends Cell Biol. 1998 Aug;8(8):310–318. [PubMed]
  • Heald R, Walczak CE. Microtubule-based motor function in mitosis. Curr Opin Struct Biol. 1999 Apr;9(2):268–274. [PubMed]
  • Hirokawa N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science. 1998 Jan 23;279(5350):519–526. [PubMed]
  • Lee YM, Lee S, Lee E, Shin H, Hahn H, Choi W, Kim W. Human kinesin superfamily member 4 is dominantly localized in the nuclear matrix and is associated with chromosomes during mitosis. Biochem J. 2001 Dec 15;360(Pt 3):549–556. [PMC free article] [PubMed]
  • Pederson T. Half a century of "the nuclear matrix". Mol Biol Cell. 2000 Mar;11(3):799–805. [PMC free article] [PubMed]
  • Marmorstein LY, Kinev AV, Chan GK, Bochar DA, Beniya H, Epstein JA, Yen TJ, Shiekhattar R. A human BRCA2 complex containing a structural DNA binding component influences cell cycle progression. Cell. 2001 Jan 26;104(2):247–257. [PubMed]
  • Lee Young Mi, Shin Hyunjin, Choi Wonja, Ahn Sungmin, Kim Wankee. Characterization of human SMARCE1r high-mobility-group protein. Biochim Biophys Acta. 2002 Apr 12;1574(3):269–276. [PubMed]
  • Evan GI, Hancock DC. Studies on the interaction of the human c-myc protein with cell nuclei: p62c-myc as a member of a discrete subset of nuclear proteins. Cell. 1985 Nov;43(1):253–261. [PubMed]
  • Sekine Y, Okada Y, Noda Y, Kondo S, Aizawa H, Takemura R, Hirokawa N. A novel microtubule-based motor protein (KIF4) for organelle transports, whose expression is regulated developmentally. J Cell Biol. 1994 Oct;127(1):187–201. [PMC free article] [PubMed]
  • Verhey KJ, Rapoport TA. Kinesin carries the signal. Trends Biochem Sci. 2001 Sep;26(9):545–550. [PubMed]
  • Karcher Ryan L, Deacon Sean W, Gelfand Vladimir I. Motor-cargo interactions: the key to transport specificity. Trends Cell Biol. 2002 Jan;12(1):21–27. [PubMed]
  • Meluh PB, Rose MD. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 1990 Mar 23;60(6):1029–1041. [PubMed]
  • Saunders W, Hornack D, Lengyel V, Deng C. The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules. J Cell Biol. 1997 Apr 21;137(2):417–431. [PMC free article] [PubMed]
  • Cottingham FR, Gheber L, Miller DL, Hoyt MA. Novel roles for saccharomyces cerevisiae mitotic spindle motors. J Cell Biol. 1999 Oct 18;147(2):335–350. [PMC free article] [PubMed]
  • Page BD, Satterwhite LL, Rose MD, Snyder M. Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein. J Cell Biol. 1994 Feb;124(4):507–519. [PMC free article] [PubMed]
  • Manning BD, Barrett JG, Wallace JA, Granok H, Snyder M. Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p. J Cell Biol. 1999 Mar 22;144(6):1219–1233. [PMC free article] [PubMed]
  • Barrett JG, Manning BD, Snyder M. The Kar3p kinesin-related protein forms a novel heterodimeric structure with its associated protein Cik1p. Mol Biol Cell. 2000 Jul;11(7):2373–2385. [PMC free article] [PubMed]
  • Hakimi Mohamed-Ali, Bochar Daniel A, Chenoweth Josh, Lane William S, Mandel Gail, Shiekhattar Ramin. A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes. Proc Natl Acad Sci U S A. 2002 May 28;99(11):7420–7425. [PMC free article] [PubMed]
  • Wang W, Côté J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M, et al. Purification and biochemical heterogeneity of the mammalian SWI-SNF complex. EMBO J. 1996 Oct 1;15(19):5370–5382. [PMC free article] [PubMed]
  • Kadonaga JT. Eukaryotic transcription: an interlaced network of transcription factors and chromatin-modifying machines. Cell. 1998 Feb 6;92(3):307–313. [PubMed]
  • Struhl K. Histone acetylation and transcriptional regulatory mechanisms. Genes Dev. 1998 Mar 1;12(5):599–606. [PubMed]
  • Kozielski F, Sack S, Marx A, Thormählen M, Schönbrunn E, Biou V, Thompson A, Mandelkow EM, Mandelkow E. The crystal structure of dimeric kinesin and implications for microtubule-dependent motility. Cell. 1997 Dec 26;91(7):985–994. [PubMed]
  • Hirokawa N. Stirring up development with the heterotrimeric kinesin KIF3. Traffic. 2000 Jan;1(1):29–34. [PubMed]
  • Burkhard P, Stetefeld J, Strelkov SV. Coiled coils: a highly versatile protein folding motif. Trends Cell Biol. 2001 Feb;11(2):82–88. [PubMed]
  • Sutton RB, Fasshauer D, Jahn R, Brunger AT. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution. Nature. 1998 Sep 24;395(6700):347–353. [PubMed]

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