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Biochem J. Oct 1, 2000; 351(Pt 1): 107–114.
PMCID: PMC1221340

A novel peroxiredoxin of the plant Sedum lineare is a homologue of Escherichia coli bacterioferritin co-migratory protein (Bcp).

Abstract

We cloned a gene encoding a 17-kDa protein from a cDNA library of the plant Sedum lineare and found that its deduced amino acid sequence showed similarities to those of Escherichia coli bacterioferritin co-migratory protein (Bcp) and its homologues, which comprise a discrete group associated with the peroxiredoxin (Prx) family. Studies of the recombinant 17-kDa protein produced in E. coli cells revealed that it actually had a thioredoxin-dependent peroxidase activity, the hallmark of the Prx family. PrxQ, as we now designate the 17-kDa protein, had two cysteine residues (Cys-44 and Cys-49) well conserved among proteins of the Bcp group. These two cysteines were demonstrated to be essential for the thioredoxin-dependent peroxidase activity by analysis of mutant proteins, suggesting that these residues are involved in the formation of an intramolecular disulphide bond as an intermediate in the reaction cycle. Expression of PrxQ suppressed the hypersensitivity of an E. coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Fridovich I. The biology of oxygen radicals. Science. 1978 Sep 8;201(4359):875–880. [PubMed]
  • Yu BP. Cellular defenses against damage from reactive oxygen species. Physiol Rev. 1994 Jan;74(1):139–162. [PubMed]
  • Mignotte B, Vayssiere JL. Mitochondria and apoptosis. Eur J Biochem. 1998 Feb 15;252(1):1–15. [PubMed]
  • Sies H. Strategies of antioxidant defense. Eur J Biochem. 1993 Jul 15;215(2):213–219. [PubMed]
  • Jacobson FS, Morgan RW, Christman MF, Ames BN. An alkyl hydroperoxide reductase from Salmonella typhimurium involved in the defense of DNA against oxidative damage. Purification and properties. J Biol Chem. 1989 Jan 25;264(3):1488–1496. [PubMed]
  • Chae HZ, Robison K, Poole LB, Church G, Storz G, Rhee SG. Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7017–7021. [PMC free article] [PubMed]
  • Chae HZ, Chung SJ, Rhee SG. Thioredoxin-dependent peroxide reductase from yeast. J Biol Chem. 1994 Nov 4;269(44):27670–27678. [PubMed]
  • Kwon SJ, Park JW, Choi WK, Kim IH, Kim K. Inhibition of metal-catalyzed oxidation systems by a yeast protector protein in the presence of thioredoxin. Biochem Biophys Res Commun. 1994 May 30;201(1):8–15. [PubMed]
  • Verdoucq L, Vignols F, Jacquot JP, Chartier Y, Meyer Y. In vivo characterization of a thioredoxin h target protein defines a new peroxiredoxin family. J Biol Chem. 1999 Jul 9;274(28):19714–19722. [PubMed]
  • Neidhardt FC, Vaughn V, Phillips TA, Bloch PL. Gene-protein index of Escherichia coli K-12. Microbiol Rev. 1983 Jun;47(2):231–284. [PMC free article] [PubMed]
  • Andrews SC, Harrison PM, Guest JR. A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map. J Gen Microbiol. 1991 Feb;137(2):361–367. [PubMed]
  • Jeong W, Cha MK, Kim IH. Thioredoxin-dependent hydroperoxide peroxidase activity of bacterioferritin comigratory protein (BCP) as a new member of the thiol-specific antioxidant protein (TSA)/Alkyl hydroperoxide peroxidase C (AhpC) family. J Biol Chem. 2000 Jan 28;275(4):2924–2930. [PubMed]
  • Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. [PubMed]
  • Messing J. New M13 vectors for cloning. Methods Enzymol. 1983;101:20–78. [PubMed]
  • Deng WP, Nickoloff JA. Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal Biochem. 1992 Jan;200(1):81–88. [PubMed]
  • Vestweber D, Schatz G. Mitochondria can import artificial precursor proteins containing a branched polypeptide chain or a carboxy-terminal stilbene disulfonate. J Cell Biol. 1988 Dec;107(6 Pt 1):2045–2049. [PMC free article] [PubMed]
  • Kobayashi T, Ito K. Respiratory chain strongly oxidizes the CXXC motif of DsbB in the Escherichia coli disulfide bond formation pathway. EMBO J. 1999 Mar 1;18(5):1192–1198. [PMC free article] [PubMed]
  • Kang SW, Baines IC, Rhee SG. Characterization of a mammalian peroxiredoxin that contains one conserved cysteine. J Biol Chem. 1998 Mar 13;273(11):6303–6311. [PubMed]
  • Jeong JS, Kwon SJ, Kang SW, Rhee SG, Kim K. Purification and characterization of a second type thioredoxin peroxidase (type II TPx) from Saccharomyces cerevisiae. Biochemistry. 1999 Jan 12;38(2):776–783. [PubMed]
  • Chae HZ, Uhm TB, Rhee SG. Dimerization of thiol-specific antioxidant and the essential role of cysteine 47. Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7022–7026. [PMC free article] [PubMed]
  • Hirotsu S, Abe Y, Okada K, Nagahara N, Hori H, Nishino T, Hakoshima T. Crystal structure of a multifunctional 2-Cys peroxiredoxin heme-binding protein 23 kDa/proliferation-associated gene product. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12333–12338. [PMC free article] [PubMed]
  • Knoops B, Clippe A, Bogard C, Arsalane K, Wattiez R, Hermans C, Duconseille E, Falmagne P, Bernard A. Cloning and characterization of AOEB166, a novel mammalian antioxidant enzyme of the peroxiredoxin family. J Biol Chem. 1999 Oct 22;274(43):30451–30458. [PubMed]
  • Lee TH, Yu SL, Kim SU, Kim YM, Choi I, Kang SW, Rhee SG, Yu DY. Characterization of the murine gene encoding 1-Cys peroxiredoxin and identification of highly homologous genes. Gene. 1999 Jul 8;234(2):337–344. [PubMed]
  • Lee J, Spector D, Godon C, Labarre J, Toledano MB. A new antioxidant with alkyl hydroperoxide defense properties in yeast. J Biol Chem. 1999 Feb 19;274(8):4537–4544. [PubMed]
  • Schmidt GW, Mishkind ML. The transport of proteins into chloroplasts. Annu Rev Biochem. 1986;55:879–912. [PubMed]
  • Douwe de Boer A, Weisbeek PJ. Chloroplast protein topogenesis: import, sorting and assembly. Biochim Biophys Acta. 1991 Nov 13;1071(3):221–253. [PubMed]
  • Kallis GB, Holmgren A. Differential reactivity of the functional sulfhydryl groups of cysteine-32 and cysteine-35 present in the reduced form of thioredoxin from Escherichia coli. J Biol Chem. 1980 Nov 10;255(21):10261–10265. [PubMed]
  • Wynn R, Cocco MJ, Richards FM. Mixed disulfide intermediates during the reduction of disulfides by Escherichia coli thioredoxin. Biochemistry. 1995 Sep 19;34(37):11807–11813. [PubMed]
  • Martin JL. Thioredoxin--a fold for all reasons. Structure. 1995 Mar 15;3(3):245–250. [PubMed]
  • Chivers PT, Laboissière MC, Raines RT. The CXXC motif: imperatives for the formation of native disulfide bonds in the cell. EMBO J. 1996 Jun 3;15(11):2659–2667. [PMC free article] [PubMed]
  • Gilbert HF. Thiol/disulfide exchange equilibria and disulfide bond stability. Methods Enzymol. 1995;251:8–28. [PubMed]
  • Gutierrez-Marcos JF, Roberts MA, Campbell EI, Wray JL. Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and "APS reductase" activity. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13377–13382. [PMC free article] [PubMed]
  • Setya A, Murillo M, Leustek T. Sulfate reduction in higher plants: molecular evidence for a novel 5'-adenylylsulfate reductase. Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13383–13388. [PMC free article] [PubMed]
  • Mustacich D, Powis G. Thioredoxin reductase. Biochem J. 2000 Feb 15;346(Pt 1):1–8. [PMC free article] [PubMed]
  • Montemartini M, Nogoceke E, Singh M, Steinert P, Flohé L, Kalisz HM. Sequence analysis of the tryparedoxin peroxidase gene from Crithidia fasciculata and its functional expression in Escherichia coli. J Biol Chem. 1998 Feb 27;273(9):4864–4871. [PubMed]

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