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Biochem J. Aug 15, 2000; 350(Pt 1): 313–319.
PMCID: PMC1221257

Oxygen and haem regulate the synthesis of peroxisomal proteins: catalase A, acyl-CoA oxidase and Pex1p in the yeast Saccharomyces cerevisiae; the regulation of these proteins by oxygen is not mediated by haem.

Abstract

Saccharomyces cerevisiae genes related to respiration are typically controlled by oxygen and haem. Usually the regulation by these factors is co-ordinated; haem is indicated as the oxygen sensor. However, the responsiveness of peroxisome functions to these regulatory factors is poorly understood. The expression of CTA1, POX1 and PEX1 genes encoding the peroxisomal proteins catalase A, acyl-CoA oxidase and Pex1p peroxin respectively was studied under various conditions: in anaerobiosis, in the absence of haem and in respiratory incompetence caused by the lack of a mitochondrial genome (rho(0)). The influence of haem deficiency or rho(0) on peroxisomal morphology was also investigated. Respiratory incompetence has no effect on the expression of CTA1 and POX1, whereas in the absence of haem their expression is markedly decreased. The synthesis of Pex1p is decreased in rho(0) cells and is decreased even more in haem-deficient cells. Nevertheless, peroxisomal morphology in both these types of cell does not differ significantly from the morphology of peroxisomes in wild-type cells. The down-regulating effect of anoxia on the expression of CTA1 and POX1 is even stronger than the effect of haem deficiency and is not reversed by the addition of exogenous haem or the presence of endogenous haem. Moreover, neither of these genes responds to the known haem-controlled transcriptional factor Hap1p. In contrast with the other two genes studied, PEX1 is up-regulated in anaerobiosis. The existence of one or more novel mechanisms of regulation of peroxisomal genes by haem and oxygen, different from those already known in S. cerevisiae, is postulated.

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Selected References

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  • Zitomer RS, Lowry CV. Regulation of gene expression by oxygen in Saccharomyces cerevisiae. Microbiol Rev. 1992 Mar;56(1):1–11. [PMC free article] [PubMed]
  • Zitomer RS, Carrico P, Deckert J. Regulation of hypoxic gene expression in yeast. Kidney Int. 1997 Feb;51(2):507–513. [PubMed]
  • Bunn HF, Poyton RO. Oxygen sensing and molecular adaptation to hypoxia. Physiol Rev. 1996 Jul;76(3):839–885. [PubMed]
  • Kwast KE, Burke PV, Poyton RO. Oxygen sensing and the transcriptional regulation of oxygen-responsive genes in yeast. J Exp Biol. 1998 Apr;201(Pt 8):1177–1195. [PubMed]
  • Verdière J, Gaisne M, Labbe-Bois R. CYP1 (HAP1) is a determinant effector of alternative expression of heme-dependent transcribed genes in yeast [corrected]. Mol Gen Genet. 1991 Aug;228(1-2):300–306. [PubMed]
  • Balasubramanian B, Lowry CV, Zitomer RS. The Rox1 repressor of the Saccharomyces cerevisiae hypoxic genes is a specific DNA-binding protein with a high-mobility-group motif. Mol Cell Biol. 1993 Oct;13(10):6071–6078. [PMC free article] [PubMed]
  • Kwast KE, Burke PV, Staahl BT, Poyton RO. Oxygen sensing in yeast: evidence for the involvement of the respiratory chain in regulating the transcription of a subset of hypoxic genes. Proc Natl Acad Sci U S A. 1999 May 11;96(10):5446–5451. [PMC free article] [PubMed]
  • Skoneczny M, Rytka J. Maintenance of the peroxisomal compartment in glucose-repressed and anaerobically grown Saccharomyces cerevisiae cells. Biochimie. 1996;78(2):95–102. [PubMed]
  • Kunau WH. Peroxisome biogenesis: from yeast to man. Curr Opin Microbiol. 1998 Apr;1(2):232–237. [PubMed]
  • Rothstein R. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. Methods Enzymol. 1991;194:281–301. [PubMed]
  • Simon M, Adam G, Rapatz W, Spevak W, Ruis H. The Saccharomyces cerevisiae ADR1 gene is a positive regulator of transcription of genes encoding peroxisomal proteins. Mol Cell Biol. 1991 Feb;11(2):699–704. [PMC free article] [PubMed]
  • Erdmann R, Wiebel FF, Flessau A, Rytka J, Beyer A, Fröhlich KU, Kunau WH. PAS1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative ATPases. Cell. 1991 Feb 8;64(3):499–510. [PubMed]
  • Cubitt AB, Heim R, Adams SR, Boyd AE, Gross LA, Tsien RY. Understanding, improving and using green fluorescent proteins. Trends Biochem Sci. 1995 Nov;20(11):448–455. [PubMed]
  • Gietz RD, Schiestl RH, Willems AR, Woods RA. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast. 1995 Apr 15;11(4):355–360. [PubMed]
  • Osumi T, Hashimoto T. Acyl-CoA oxidase of rat liver: a new enzyme for fatty acid oxidation. Biochem Biophys Res Commun. 1978 Jul 28;83(2):479–485. [PubMed]
  • BEERS RF, Jr, SIZER IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952 Mar;195(1):133–140. [PubMed]
  • Guarente L. Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast. Methods Enzymol. 1983;101:181–191. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Skoneczny M, Chełstowska A, Rytka J. Study of the coinduction by fatty acids of catalase A and acyl-CoA oxidase in standard and mutant Saccharomyces cerevisiae strains. Eur J Biochem. 1988 Jun 1;174(2):297–302. [PubMed]
  • Simon M, Binder M, Adam G, Hartig A, Ruis H. Control of peroxisome proliferation in Saccharomyces cerevisiae by ADR1, SNF1 (CAT1, CCR1) and SNF4 (CAT3). Yeast. 1992 Apr;8(4):303–309. [PubMed]
  • Chelstowska A, Butow RA. RTG genes in yeast that function in communication between mitochondria and the nucleus are also required for expression of genes encoding peroxisomal proteins. J Biol Chem. 1995 Jul 28;270(30):18141–18146. [PubMed]
  • Hörtner H, Ammerer G, Hartter E, Hamilton B, Rytka J, Bilinski T, Ruis H. Regulation of synthesis of catalases and iso-1-cytochrome c in Saccharomyces cerevisiae by glucose, oxygen and heme. Eur J Biochem. 1982 Nov;128(1):179–184. [PubMed]
  • Purdue PE, Yang X, Lazarow PB. Pex18p and Pex21p, a novel pair of related peroxins essential for peroxisomal targeting by the PTS2 pathway. J Cell Biol. 1998 Dec 28;143(7):1859–1869. [PMC free article] [PubMed]
  • Liao X, Butow RA. RTG1 and RTG2: two yeast genes required for a novel path of communication from mitochondria to the nucleus. Cell. 1993 Jan 15;72(1):61–71. [PubMed]
  • Lowry CV, Lieber RH. Negative regulation of the Saccharomyces cerevisiae ANB1 gene by heme, as mediated by the ROX1 gene product. Mol Cell Biol. 1986 Dec;6(12):4145–4148. [PMC free article] [PubMed]
  • Hodge MR, Kim G, Singh K, Cumsky MG. Inverse regulation of the yeast COX5 genes by oxygen and heme. Mol Cell Biol. 1989 May;9(5):1958–1964. [PMC free article] [PubMed]
  • Winkler H, Adam G, Mattes E, Schanz M, Hartig A, Ruis H. Co-ordinate control of synthesis of mitochondrial and non-mitochondrial hemoproteins: a binding site for the HAP1 (CYP1) protein in the UAS region of the yeast catalase T gene (CTT1). EMBO J. 1988 Jun;7(6):1799–1804. [PMC free article] [PubMed]
  • King DA, Zhang L, Guarente L, Marmorstein R. Structure of a HAP1-DNA complex reveals dramatically asymmetric DNA binding by a homodimeric protein. Nat Struct Biol. 1999 Jan;6(1):64–71. [PubMed]
  • Verdière J, Creusot F, Guarente L, Slonimski PP. The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c. Curr Genet. 1986;10(5):339–342. [PubMed]
  • Spevak W, Hartig A, Meindl P, Ruis H. Heme control region of the catalase T gene of the yeast Saccharomyces cerevisiae. Mol Gen Genet. 1986 Apr;203(1):73–78. [PubMed]

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