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Biochem J. May 1, 2000; 347(Pt 3): 763–770.
PMCID: PMC1221014

Identification of abundant mRNAs from the third stage larvae of the parasitic nematode, ostertagia ostertagi.


Reverse transcriptase-PCR (RT-PCR) was carried out on total RNA prepared from the third-stage larvae (L3) of Ostertagia ostertagi in order to clone and characterize the major transcripts expressed in this larval stage, as an initial investigation of arrested larval development in the parasite. Distinct bands were visible on an agarose gel and four of these were cloned and sequenced. Three of the bands represented multiple transcripts, while the fourth band encoded the enzyme GTP cyclohydrolase I (GTP-CH), which catalyses the first and rate-limiting step in pteridine biosynthesis. Northern blot analysis and RT-PCR demonstrated that GTP-CH is highly up-regulated in the L3 stage and undetectable in either the L2 or adult stages. Using immunogold electron microscopy, GTP-CH was shown to be predominantly localized to the cell body of the body wall muscles and the cells of the intestine in the L3.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Michel JF. Arrested development of nematodes and some related phenomena. Adv Parasitol. 1974;12:279–366. [PubMed]
  • Hotez P, Hawdon J, Schad GA. Hookworm larval infectivity, arrest and amphiparatenesis: the Caenorhabditis elegans Daf-c paradigm. Parasitol Today. 1993 Jan;9(1):23–26. [PubMed]
  • Armour J, Duncan M. Arrested larval development in cattle nematodes. Parasitol Today. 1987 Jun;3(6):171–176. [PubMed]
  • Eysker M. Some aspects of inhibited development of trichostrongylids in ruminants. Vet Parasitol. 1997 Nov;72(3-4):265–283. [PubMed]
  • Michel JF, Lancaster MB, Hong C. The effect of age, acquired resistance, pregnancy and lactation on some reactions of cattle to infection with Ostertagia ostertagi. Parasitology. 1979 Aug;79(1):157–168. [PubMed]
  • Nilsen TW. Trans-splicing of nematode premessenger RNA. Annu Rev Microbiol. 1993;47:413–440. [PubMed]
  • McKeand JB, Kennedy MW. Shedding of surface-bound antibody by adult Dictyocaulus viviparus. Int J Parasitol. 1995 Oct;25(10):1255–1258. [PubMed]
  • Krause M, Wild M, Rosenzweig B, Hirsh D. Wild-type and mutant actin genes in Caenorhabditis elegans. J Mol Biol. 1989 Aug 5;208(3):381–392. [PubMed]
  • Lewis E, Hunter SJ, Tetley L, Nunes CP, Bazzicalupo P, Devaney E. cut-1-like genes are present in the filarial nematodes, Brugia pahangi and Brugia malayi, and, as in other nematodes, code for components of the cuticle. Mol Biochem Parasitol. 1999 Jun 25;101(1-2):173–183. [PubMed]
  • Cigan AM, Donahue TF. Sequence and structural features associated with translational initiator regions in yeast--a review. Gene. 1987;59(1):1–18. [PubMed]
  • Nar H, Huber R, Meining W, Schmid C, Weinkauf S, Bacher A. Atomic structure of GTP cyclohydrolase I. Structure. 1995 May 15;3(5):459–466. [PubMed]
  • Hatakeyama K, Inoue Y, Harada T, Kagamiyama H. Cloning and sequencing of cDNA encoding rat GTP cyclohydrolase I. The first enzyme of the tetrahydrobiopterin biosynthetic pathway. J Biol Chem. 1991 Jan 15;266(2):765–769. [PubMed]
  • Fling ME, Kopf J, Richards CA. Nucleotide sequence of the dihydrofolate reductase gene of Saccharomyces cerevisiae. Gene. 1988 Mar 31;63(2):165–174. [PubMed]
  • Sebastiano M, Lassandro F, Bazzicalupo P. cut-1 a Caenorhabditis elegans gene coding for a dauer-specific noncollagenous component of the cuticle. Dev Biol. 1991 Aug;146(2):519–530. [PubMed]
  • Blaxter ML, Raghavan N, Ghosh I, Guiliano D, Lu W, Williams SA, Slatko B, Scott AL. Genes expressed in Brugia malayi infective third stage larvae. Mol Biochem Parasitol. 1996 Apr;77(1):77–93. [PubMed]
  • Mackay WJ, O'Donnell JM. A genetic analysis of the pteridine biosynthetic enzyme, guanosine triphosphate cyclohydrolase, in Drosophila melanogaster. Genetics. 1983 Sep;105(1):35–53. [PMC free article] [PubMed]
  • Ichinose H, Ohye T, Takahashi E, Seki N, Hori T, Segawa M, Nomura Y, Endo K, Tanaka H, Tsuji S, et al. Hereditary progressive dystonia with marked diurnal fluctuation caused by mutations in the GTP cyclohydrolase I gene. Nat Genet. 1994 Nov;8(3):236–242. [PubMed]
  • Niederwieser A, Blau N, Wang M, Joller P, Atarés M, Cardesa-Garcia J. GTP cyclohydrolase I deficiency, a new enzyme defect causing hyperphenylalaninemia with neopterin, biopterin, dopamine, and serotonin deficiencies and muscular hypotonia. Eur J Pediatr. 1984 Feb;141(4):208–214. [PubMed]
  • de la Fuente-Fernández R. Mutations in GTP-cyclohydrolase I gene and vitiligo. Lancet. 1997 Aug 30;350(9078):640–640. [PubMed]
  • Miwa S, Watanabe Y, Hayaishi O. 6R-L-erythro-5,6,7,8-tetrahydrobiopterin as a regulator of dopamine and serotonin biosynthesis in the rat brain. Arch Biochem Biophys. 1985 May 15;239(1):234–241. [PubMed]
  • Tanaka K, Kaufman S, Milstien S. Tetrahydrobiopterin, the cofactor for aromatic amino acid hydroxylases, is synthesized by and regulates proliferation of erythroid cells. Proc Natl Acad Sci U S A. 1989 Aug;86(15):5864–5867. [PMC free article] [PubMed]
  • Brown GM, Williamson JM. Biosynthesis of riboflavin, folic acid, thiamine, and pantothenic acid. Adv Enzymol Relat Areas Mol Biol. 1982;53:345–381. [PubMed]
  • Schott K, Gütlich M, Ziegler I. Induction of GTP-cyclohydrolase I mRNA expression by lectin activation and interferon-gamma treatment in human cells associated with the immune response. J Cell Physiol. 1993 Jul;156(1):12–16. [PubMed]
  • Hatakeyama K, Harada T, Kagamiyama H. IMP dehydrogenase inhibitors reduce intracellular tetrahydrobiopterin levels through reduction of intracellular GTP levels. Indications of the regulation of GTP cyclohydrolase I activity by restriction of GTP availability in the cells. J Biol Chem. 1992 Oct 15;267(29):20734–20739. [PubMed]
  • Milstien S, Jaffe H, Kowlessur D, Bonner TI. Purification and cloning of the GTP cyclohydrolase I feedback regulatory protein, GFRP. J Biol Chem. 1996 Aug 16;271(33):19743–19751. [PubMed]
  • Reynolds ER, O'Donnell JM. An analysis of the embryonic defects in Punch mutants of Drosophila melanogaster. Dev Biol. 1987 Oct;123(2):430–441. [PubMed]

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