• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Oct 1, 1999; 343(Pt 1): 185–190.
PMCID: PMC1220540

Barley (Hordeum vulgare) oxalate oxidase is a manganese-containing enzyme.

Abstract

Oxalate oxidase (EC 1.2.3.4) catalyses the conversion of oxalate and dioxygen into CO(2) and H(2)O(2). The barley (Hordeum vulgare) seedling root enzyme was purified to homogeneity and shown by metal analysis and EPR spectroscopy to contain Mn(II) at up to 0.80 atom per subunit. The involvement of Mn and neither flavin, Cu nor Fe in the direct conversion of dioxygen to H(2)O(2) makes oxalate oxidase unique. A model of the active site of the holoenzyme based on a homology model of the apoenzyme is proposed.

Full Text

The Full Text of this article is available as a PDF (152K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Lane BG. Oxalate, germin, and the extracellular matrix of higher plants. FASEB J. 1994 Mar 1;8(3):294–301. [PubMed]
  • Kotsira VP, Clonis YD. Oxalate oxidase from barley roots: purification to homogeneity and study of some molecular, catalytic, and binding properties. Arch Biochem Biophys. 1997 Apr 15;340(2):239–249. [PubMed]
  • Lane BG, Dunwell JM, Ray JA, Schmitt MR, Cuming AC. Germin, a protein marker of early plant development, is an oxalate oxidase. J Biol Chem. 1993 Jun 15;268(17):12239–12242. [PubMed]
  • Dumas B, Sailland A, Cheviet JP, Freyssinet G, Pallett K. Identification of barley oxalate oxidase as a germin-like protein. C R Acad Sci III. 1993 Aug;316(8):793–798. [PubMed]
  • Dunwell JM. Cupins: a new superfamily of functionally diverse proteins that include germins and plant storage proteins. Biotechnol Genet Eng Rev. 1998;15:1–32. [PubMed]
  • Jaikaran AS, Kennedy TD, Dratewka-Kos E, Lane BG. Covalently bonded and adventitious glycans in germin. J Biol Chem. 1990 Jul 25;265(21):12503–12512. [PubMed]
  • Woo EJ, Dunwell JM, Goodenough PW, Pickersgill RW. Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X-ray crystallography. FEBS Lett. 1998 Oct 16;437(1-2):87–90. [PubMed]
  • Dumas B, Freyssinet G, Pallett KE. Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings. Plant Physiol. 1995 Apr;107(4):1091–1096. [PMC free article] [PubMed]
  • Holm Richard H, Kennepohl Pierre, Solomon Edward I. Structural and Functional Aspects of Metal Sites in Biology. Chem Rev. 1996 Nov 7;96(7):2239–2314. [PubMed]
  • Halliwell B. Non-enzymic catalysis of oxalate decarboxylation by light and flavins. Biochem J. 1972 Sep;129(2):497–498. [PMC free article] [PubMed]
  • Dunwell JM, Gane PJ. Microbial relatives of seed storage proteins: conservation of motifs in a functionally diverse superfamily of enzymes . J Mol Evol. 1998 Feb;46(2):147–154. [PubMed]
  • Gane PJ, Dunwell JM, Warwicker J. Modeling based on the structure of vicilins predicts a histidine cluster in the active site of oxalate oxidase. J Mol Evol. 1998 Apr;46(4):488–493. [PubMed]
  • Satyapal, Pundir CS. Purification and properties of an oxalate oxidase from leaves of grain sorghum hybrid CSH-5. Biochim Biophys Acta. 1993 Jan 15;1161(1):1–5. [PubMed]
  • Ko TP, Ng JD, McPherson A. The three-dimensional structure of canavalin from jack bean (Canavalia ensiformis). Plant Physiol. 1993 Mar;101(3):729–744. [PMC free article] [PubMed]
  • Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994 Nov 11;22(22):4673–4680. [PMC free article] [PubMed]
  • Rost B. PHD: predicting one-dimensional protein structure by profile-based neural networks. Methods Enzymol. 1996;266:525–539. [PubMed]
  • Lawrence MC, Izard T, Beuchat M, Blagrove RJ, Colman PM. Structure of phaseolin at 2.2 A resolution. Implications for a common vicilin/legumin structure and the genetic engineering of seed storage proteins. J Mol Biol. 1994 May 20;238(5):748–776. [PubMed]
  • Guex N, Peitsch MC. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis. 1997 Dec;18(15):2714–2723. [PubMed]
  • Chiriboga J. Purification and properties of oxalic acid oxidase. Arch Biochem Biophys. 1966 Sep 26;116(1):516–523. [PubMed]
  • Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319–326. [PubMed]
  • Kotsira VP, Clonis YD. Chemical modification of barley root oxalate oxidase shows the presence of a lysine, a carboxylate, and disulfides, essential for enzyme activity. Arch Biochem Biophys. 1998 Aug 15;356(2):117–126. [PubMed]
  • Hsieh Y, Guan Y, Tu C, Bratt PJ, Angerhofer A, Lepock JR, Hickey MJ, Tainer JA, Nick HS, Silverman DN. Probing the active site of human manganese superoxide dismutase: the role of glutamine 143. Biochemistry. 1998 Apr 7;37(14):4731–4739. [PubMed]
  • Fee JA, Shapiro ER, Moss TH. Direct evidence for manganese (III) binding to the manganosuperoxide dismutase of Escherichia coli B. J Biol Chem. 1976 Oct 10;251(19):6157–6159. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...