• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of biochemjBJ Latest papers and much more!
Biochem J. Aug 1, 1999; 341(Pt 3): 785–794.
PMCID: PMC1220419

Solution structure of a defensin-like peptide from platypus venom.

Abstract

Three defensin-like peptides (DLPs) were isolated from platypus venom and sequenced. One of these peptides, DLP-1, was synthesized chemically and its three-dimensional structure was determined using NMR spectroscopy. The main structural elements of this 42-residue peptide were an anti-parallel beta-sheet comprising residues 15-18 and 37-40 and a small 3(10) helix spanning residues 10-12. The overall three-dimensional fold is similar to that of beta-defensin-12, and similar to the sodium-channel neurotoxin ShI (Stichodactyla helianthus neurotoxin I). However, the side chains known to be functionally important in beta-defensin-12 and ShI are not conserved in DLP-1, suggesting that it has a different biological function. Consistent with this contention, we showed that DLP-1 possesses no anti-microbial properties and has no observable activity on rat dorsal-root-ganglion sodium-channel currents.

Full Text

The Full Text of this article is available as a PDF (286K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Fenner PJ, Williamson JA, Myers D. Platypus envenomation--a painful learning experience. Med J Aust. 1992 Dec 7;157(11-12):829–832. [PubMed]
  • de Plater G, Martin RL, Milburn PJ. A pharmacological and biochemical investigation of the venom from the platypus (Ornithorhynchus anatinus). Toxicon. 1995 Feb;33(2):157–169. [PubMed]
  • de Plater GM, Martin RL, Milburn PJ. The natriuretic peptide (ovCNP-39) from platypus (Ornithorhynchus anatinus) venom relaxes the isolated rat uterus and promotes oedema and mast cell histamine release. Toxicon. 1998 Jun;36(6):847–857. [PubMed]
  • Craik D, Munro S, Nielsen K, Shehan P, Tregear G, Wade J. The conformation of porcine-brain natriuretic peptide by two-dimensional NMR spectroscopy. Eur J Biochem. 1991 Oct 1;201(1):183–190. [PubMed]
  • Inooka H, Kikuchi T, Endo S, Ishibashi Y, Wakimasu M, Mizuta E. Conformation in solution of porcine brain natriuretic peptide determined by combined use of nuclear magnetic resonance and distance geometry. Eur J Biochem. 1990 Oct 5;193(1):127–134. [PubMed]
  • Weber J, Rösch P, Adermann K, Forssmann WG, Wokaun A. 1H-NMR studies of the natriuretic peptide urodilatin: sequence-specific resonance assignment. Biochim Biophys Acta. 1994 Aug 17;1207(2):231–235. [PubMed]
  • Selsted ME, Tang YQ, Morris WL, McGuire PA, Novotny MJ, Smith W, Henschen AH, Cullor JS. Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. J Biol Chem. 1993 Mar 25;268(9):6641–6648. [PubMed]
  • Nicholson GM, Willow M, Howden ME, Narahashi T. Modification of sodium channel gating and kinetics by versutoxin from the Australian funnel-web spider Hadronyche versuta. Pflugers Arch. 1994 Oct;428(3-4):400–409. [PubMed]
  • Marion D, Wüthrich K. Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins. Biochem Biophys Res Commun. 1983 Jun 29;113(3):967–974. [PubMed]
  • Rance M, Sørensen OW, Bodenhausen G, Wagner G, Ernst RR, Wüthrich K. Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering. Biochem Biophys Res Commun. 1983 Dec 16;117(2):479–485. [PubMed]
  • Piotto M, Saudek V, Sklenár V. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol NMR. 1992 Nov;2(6):661–665. [PubMed]
  • Wu W, Berkowitz GA. K stimulation of ATPase activity associated with the chloroplast inner envelope. Plant Physiol. 1992 Jun;99(2):553–560. [PMC free article] [PubMed]
  • Ludvigsen S, Poulsen FM. Positive theta-angles in proteins by nuclear magnetic resonance spectroscopy. J Biomol NMR. 1992 May;2(3):227–233. [PubMed]
  • Güntert P, Mumenthaler C, Wüthrich K. Torsion angle dynamics for NMR structure calculation with the new program DYANA. J Mol Biol. 1997 Oct 17;273(1):283–298. [PubMed]
  • Hyberts SG, Goldberg MS, Havel TF, Wagner G. The solution structure of eglin c based on measurements of many NOEs and coupling constants and its comparison with X-ray structures. Protein Sci. 1992 Jun;1(6):736–751. [PMC free article] [PubMed]
  • Hutchinson EG, Thornton JM. PROMOTIF--a program to identify and analyze structural motifs in proteins. Protein Sci. 1996 Feb;5(2):212–220. [PMC free article] [PubMed]
  • Holm L, Sander C. Protein structure comparison by alignment of distance matrices. J Mol Biol. 1993 Sep 5;233(1):123–138. [PubMed]
  • Zimmermann GR, Legault P, Selsted ME, Pardi A. Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the classical defensins. Biochemistry. 1995 Oct 17;34(41):13663–13671. [PubMed]
  • Fogh RH, Kem WR, Norton RS. Solution structure of neurotoxin I from the sea anemone Stichodactyla helianthus. A nuclear magnetic resonance, distance geometry, and restrained molecular dynamics study. J Biol Chem. 1990 Aug 5;265(22):13016–13028. [PubMed]
  • Wilcox GR, Fogh RH, Norton RS. Refined structure in solution of the sea anemone neurotoxin ShI. J Biol Chem. 1993 Nov 25;268(33):24707–24719. [PubMed]
  • Sibanda BL, Thornton JM. Beta-hairpin families in globular proteins. Nature. 1985 Jul 11;316(6024):170–174. [PubMed]
  • Gould AR, Mabbutt BC, Llewellyn LE, Goss NH, Norton RS. Linear and cyclic peptide analogues of the polypeptide cardiac stimulant, anthopleurin-A. 1H-NMR and biological activity studies. Eur J Biochem. 1992 Jun 15;206(3):641–651. [PubMed]
  • Fox JW, Elzinga M, Tu AT. Amino acid sequence and disulfide bond assignment of myotoxin a isolated from the venom of Prairie rattlesnake (Crotalus viridis viridis). Biochemistry. 1979 Feb 20;18(4):678–684. [PubMed]
  • Mebs D, Ownby CL. Myotoxic components of snake venoms: their biochemical and biological activities. Pharmacol Ther. 1990;48(2):223–236. [PubMed]
  • O'Keefe MP, Nedelkov D, Bieber AL, Nieman RA. Evidence for isomerization in myotoxin a from the prairie rattlesnake (Crotalus viridis viridis). Toxicon. 1996 Apr;34(4):417–434. [PubMed]
  • Norton RS, Pallaghy PK. The cystine knot structure of ion channel toxins and related polypeptides. Toxicon. 1998 Nov;36(11):1573–1583. [PubMed]
  • Barton GJ. ALSCRIPT: a tool to format multiple sequence alignments. Protein Eng. 1993 Jan;6(1):37–40. [PubMed]
  • Koradi R, Billeter M, Wüthrich K. MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph. 1996 Feb;14(1):51–32. [PubMed]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...