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Genetics. Aug 1981; 98(4): 691–711.
PMCID: PMC1214469

Agents That Cause a High Frequency of Genetic Change from [psi+] to [psi-] in SACCHAROMYCES CEREVISIAE

Abstract

The [psi] factor of yeast is cytoplasmically inherited. Singh, Helms and Sherman (1979) reported that high concentrations of KCl and of ethylene glycol induce the genetic change from [psi+] to [psi-]. In this study, the following agents have been shown to induce the same genetic change: guanidine hydrochloride at 1 mm, dimethyl sulfoxide at 2.5% v/v and ethanol or methanol at 10% v/v. It is likely that a number of other agents also cause the change, namely 2 m glycerol, m succinate, m glutamate and m MgCl2. Most of these agents induce the change at very high frequencies; with some, the frequency is 100%. Although the observed phenotypic change can also occur as a result of chromosomal gene mutation, no changes of this type were identified. Some of the agents also cause mutation from [rho+] to [rho-] and from killer to sensitive.

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Selected References

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  • Ames BN, Durston WE, Yamasaki E, Lee FD. Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2281–2285. [PMC free article] [PubMed]
  • Barclay BJ, Little JG. Genetic damage during thymidylate starvation in Saccharomyces cerevisiae. Mol Gen Genet. 1978 Mar 20;160(1):33–40. [PubMed]
  • Cox BS, Tuite MF, Mundy CJ. Reversion from suppression to nonsuppression in SUQ5 [psi+] strains of yeast: the classificaion of mutations. Genetics. 1980 Jul;95(3):589–609. [PMC free article] [PubMed]
  • Culbertson MR, Charnas L, Johnson MT, Fink GR. Frameshifts and frameshift suppressors in Saccharomyces cerevisiae. Genetics. 1977 Aug;86(4):745–764. [PMC free article] [PubMed]
  • EPHRUSSI B, HOTTINGUER H. On an unstable cell state in yeast. Cold Spring Harb Symp Quant Biol. 1951;16:75–85. [PubMed]
  • Evans WE, Parry JM. The genetic effects of elevated temperature in the yeast, Saccharomyces cerevisiae. Heredity (Edinb) 1975 Dec;35(3):347–359. [PubMed]
  • Fink GR, Styles CA. Curing of a killer factor in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2846–2849. [PMC free article] [PubMed]
  • Friend C, Freedman HA. Effects and possible mechanism of action of dimethylsulfoxide on Friend cell differentiation. Biochem Pharmacol. 1978 May 1;27(9):1309–1313. [PubMed]
  • Juliani MH, Gambarini AG, Costa SO. Induction of rho-minus mutants in Saccharomyces cerevisiae by guanidine hydrochloride. I. Genetic analysis. Mutat Res. 1975 Jul;29(1):67–75. [PubMed]
  • McCready SJ, Cox BS. Antisuppressors in yeast. Mol Gen Genet. 1973 Aug 28;124(4):305–320. [PubMed]
  • NAGAI S, YANAGISHIMA N, NAGAI H. Advances in the study of respiration-deficient (RD) mutation in yeast and other microorganisms. Bacteriol Rev. 1961 Dec;25:404–426. [PMC free article] [PubMed]
  • Oliver SG, Williamson DH. The molecular events involved in the induction of petite yeast mutants by fluorinated pyrimidines. Mol Gen Genet. 1976 Aug 2;146(3):253–259. [PubMed]
  • Ono BI, Stewart JW, Sherman F. Yeast UAA suppressors effective in psi+ strains serine-inserting suppressors. J Mol Biol. 1979 Feb 15;128(1):81–100. [PubMed]
  • Nakanishi S, Adhya S, Gottesman M, Pastan I. Effects of dimethylsulfoxide on the E. coli gal operon and on bacteriophage lambda in vivo. Cell. 1974 Sep;3(1):39–46. [PubMed]
  • Newlon CS, Ludescher RD, Walter SK. Production of petites by cell cycle mutants of Saccharomyces cerevisiae defective in DNA synthesis. Mol Gen Genet. 1979 Jan 31;169(2):189–194. [PubMed]
  • Putrament A, Baranowska H, Prazmo W. Induction by manganese of mitochondrial antibiotic resistance mutations in yeast. Mol Gen Genet. 1973 Nov 22;126(4):357–366. [PubMed]
  • Singh A, Helms C, Sherman F. Mutation of the non-Mendelian suppressor, Psi, in yeast by hypertonic media. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1952–1956. [PMC free article] [PubMed]
  • Singh DR, Mahajan JM, Krishnan D. Effect of dimethyl sulfoxide (DMSO) on radiation-induced heteroallelic reversion in diploid yeast. Mutat Res. 1976 Nov;37(2-3):193–200. [PubMed]
  • Slonimski PP, Perrodin G, Croft JH. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. [PubMed]
  • Wickner RB. "Killer character" of Saccharomyces cerevisiae: curing by growth at elevated temperature. J Bacteriol. 1974 Mar;117(3):1356–1357. [PMC free article] [PubMed]
  • Williamson DH, Maroudas NG, Wilkie D. Induction of the cytoplasmic petite mutation in Saccharomyces cerevisiae by the antibacterial antibiotics erythromycin and chloramphenicol. Mol Gen Genet. 1971;111(3):209–223. [PubMed]

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