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Genetics. 1980 Aug; 95(4): 1013–1022.
PMCID: PMC1214263

Biochemical Differences between Products of the Adh Locus in Drosophila

Abstract

An analysis of the molecular properties of the major alcohol dehydrogenase (E.C.1.1.1.1) allozyme variants found segregating in natural populations of D. melanogaster is presented. Our results indicate: (1) ADH-S enzyme has generally lower Michaelis-Menten constants than those of ADH-F; (2) ADH-S and ADH-F enzymes display opposite interactions for both co-factor and substrate; and (3) higher levels of ADH are associated with the Adh-fast genotype. The possible adaptive significance of these findings is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barnes BW, Birley AJ. Genetical variation for enzyme activity in a population of Drosophila melanogaster. IV. Analysis of alcohol dehydrogenase activity in chromosome substitution lines. Heredity (Edinb) 1978 Feb;40(1):51–57. [PubMed]
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. [PubMed]
  • Cavener DR, Clegg MT. Dynamics of correlated genetic systems. IV. Multilocus effects of ethanol stress environments. Genetics. 1978 Nov;90(3):629–644. [PMC free article] [PubMed]
  • Day TH, Hillier PC, Clarke B. The relative quantities and catalytic activities of enzymes produced by alleles at the alcohol dehydrogenase locus in Drosophila melanogaster. Biochem Genet. 1974 Feb;11(2):155–165. [PubMed]
  • Elliott JI, Knopp JA. Alcohol dehydrogenase from Drosophila melanogaster. Methods Enzymol. 1975;41:374–379. [PubMed]
  • Fletcher TS, Ayala FJ, Thatcher DR, Chambers GK. Structural analysis of the ADHS electromorph of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5609–5612. [PMC free article] [PubMed]
  • FLORINI JR, VESTLING CS. Graphical determination of the dissociation constants for two-substrate enzyme systems. Biochim Biophys Acta. 1957 Sep;25(3):575–578. [PubMed]
  • Gibson JB. Differences in the number of molecules produced by two allelic electrophoretic enzyme variants in D. melanogaster. Experientia. 1972 Aug 15;28(8):975–976. [PubMed]
  • Hewitt NE, Pipkin SB, Williams N, Chakrabartty PK. Variation in ADH activity in class I and class II strains of Drosophila. J Hered. 1974 May-Jun;65(3):141–148. [PubMed]
  • IMSANDE J. Pathway of diphosphopyridine nucleotide biosynthesis in Escherichia coli. J Biol Chem. 1961 May;236:1494–1497. [PubMed]
  • Laurie-Ahlberg CC, Maroni G, Bewley GC, Lucchesi JC, Weir BS. Quantitative genetic variation of enzyme activities in natural populations of Drosophila melanogaster. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1073–1077. [PMC free article] [PubMed]
  • Lewis N, Gibson J. Variation in amount of enzyme protein in natural populations. Biochem Genet. 1978 Apr;16(3-4):159–170. [PubMed]
  • Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. [PubMed]
  • Maroni G. Genetic control of alcohol dehydrogenase levels in Drosophila. Biochem Genet. 1978 Jun;16(5-6):509–523. [PubMed]
  • McDonald JF, Ayala FJ. Genetic and biochemical basis of enzyme activity variation in natural populations. I. Alcohol dehydrogenase in Drosophila melanogaster. Genetics. 1978 Jun;89(2):371–388. [PMC free article] [PubMed]
  • Schwartz M, O'Donnell J, Sofer W. Origin of the multiple forms of alcohol dehydrogenase from Drosophila melanogaster. Arch Biochem Biophys. 1979 May;194(2):365–378. [PubMed]
  • Siano DB, Zyskind JW, Fromm HJ. A computer program for fitting and statistically analyzing initial rate data applied to bovine hexokinase type III isozyme. Arch Biochem Biophys. 1975 Oct;170(2):587–600. [PubMed]

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