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Genetics. Apr 1978; 88(4): 651–671.
PMCID: PMC1213811

Characterization of a Mutation in Yeast Causing Nonrandom Chromosome Loss during Mitosis

Abstract

Diploid strains of the yeast Saccharomyces cerevisiae homozygous for a recessive chromosome loss mutation (chl) exhibit a high degree of mitotic instability. Cells become monosomic for chromosome III at a frequency of approximately one percent of all cell divisions. Chromosome loss at this high frequency is also found for chromosome I, and at lesser frequencies for chromosomes VIII and XVI. In contrast, little or no chromosome loss is found for six other linkage groups tested (II, V, VI, VII, XI and XVII). The chl mutation also induces a ten-fold increase in both intergenic and intragenic mitotic recombination on all ten linkage groups tested. The chl mutation does not cause an increase in spontaneous mutations, nor are mutant strains sensitive to UV or γ irradiation. The effects of chl during meiosis are observed primarily in reduced spore viability. A decrease in chromosome III linkage relationships is also found.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Becker HJ. The influence of heterochromatin, inversion-heterozygosity and somatic pairing on x-ray induced mitotic recombination in Drosophila melanogaster. Mol Gen Genet. 1969;105(3):203–218. [PubMed]
  • Boram WR, Roman H. Recombination in Saccharomyces cerevisiae: a DNA repair mutation associated with elevated mitotic gene conversion. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2828–2832. [PMC free article] [PubMed]
  • Campbell DA, Fogel S. Association of chromosome loss with centromere-adjacent mitotic recombination in a yeast disomic haploid. Genetics. 1977 Apr;85(4):573–585. [PMC free article] [PubMed]
  • Campbell DA, Fogel S, Lusnak K. Mitotic chromosome loss in a disomic haploid of Saccharomyces cerevisiae. Genetics. 1975 Mar;79(3):383–396. [PMC free article] [PubMed]
  • Culbertson MR, Henry SA. Genetic analysis of hybrid strains trisomic for the chromosome containing a fatty acid synthetase gene complex (fas1) in yeast. Genetics. 1973 Nov;75(3):441–458. [PMC free article] [PubMed]
  • Kaplan WD. The Influence of Minutes upon Somatic Crossing over in Drosophila Melanogaster. Genetics. 1953 Nov;38(6):630–651. [PMC free article] [PubMed]
  • Merriam JR, Nöthiger R, Garcia-Bellido A. Are dicentric anaphase bridges formed by somatic recombination in X chromosome inversion heterozygotes of Drosophila melanogaster? Mol Gen Genet. 1972;115(4):294–301. [PubMed]
  • Haber JE. Bisexual mating behavior in a diploid of Saccharomyces cerevisiae: evidence for genetically controlled non-random chromosome loss during vegetative growth. Genetics. 1974 Nov;78(3):843–858. [PMC free article] [PubMed]
  • Mortimer RK, Hawthorne DC. Genetic Mapping in Saccharomyces IV. Mapping of Temperature-Sensitive Genes and Use of Disomic Strains in Localizing Genes. Genetics. 1973 May;74(1):33–54. [PMC free article] [PubMed]
  • Stern C. Somatic Crossing over and Segregation in Drosophila Melanogaster. Genetics. 1936 Nov;21(6):625–730. [PMC free article] [PubMed]
  • WHITEHOUSE HLK. Mapping chromosome centromeres by the analysis of unordered tetrads. Nature. 1950 Jun 3;165(4205):893–893. [PubMed]
  • Wickner RB. Chromosomal and nonchromosomal mutations affecting the "killer character" of Saccharomyces cerevisiae. Genetics. 1974 Mar;76(3):423–432. [PMC free article] [PubMed]

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