• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of geneticsGeneticsCurrent IssueInformation for AuthorsEditorial BoardSubscribeSubmit a Manuscript
Genetics. Sep 1977; 87(1): 1–18.
PMCID: PMC1213719

Analysis of the Role of Recombination and Repair in Mutagenesis of ESCHERICHIA COLI by Uv Irradiation

Abstract

Multiple mutant strains have been tested for their mimicry of the UV-mutagenesis deficiency of a recA single mutant. Revertants to histidine prototrophy and clear plaque mutants of lambda were scored to determine capacity for UV-mutagenesis. Nearly normal capacity was shown by a uvr+ recB- recF - strain, which shows almost no recA-dependent recombination, by uvr- recB+ recF - strains, which show almost no recA-dependent repair and by a uvrA- recB- recF- strain, which shows neither recA-dependent recombination nor repair. Since the uvr mutants can be assumed to show additionally no excision repair, these results may mean that UV-mutagenesis occurs during processes other than recombination and repair. Alternative hypotheses are discussed. The slight difference in mutagenic capacity was traced to the recF single mutation, which blocks the production of unmixed bursts of clear-plaque lambda mutants. Since this accounts for only about 10% of the mutations leading to clear-plaque mutants, it is suggested that there is more than one UV-mutagenic process.

Full Text

The Full Text of this article is available as a PDF (1.2M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Brendel M, Haynes RH. Interactions among genes controlling sensitivity to radiation and alkylation in yeast. Mol Gen Genet. 1973 Sep 12;125(3):197–216. [PubMed]
  • Brooks K, Clark AJ. Behavior of lambda bacteriophage in a recombination deficienct strain of Escherichia coli. J Virol. 1967 Apr;1(2):283–293. [PMC free article] [PubMed]
  • Capaldo FN, Ramsey G, Barbour SD. Analysis of the growth of recombination-deficient strains of Escherichia coli K-12. J Bacteriol. 1974 Apr;118(1):242–249. [PMC free article] [PubMed]
  • Clark AJ. Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet. 1973;7:67–86. [PubMed]
  • CLARK AJ, MARGULIES AD. ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12. Proc Natl Acad Sci U S A. 1965 Feb;53:451–459. [PMC free article] [PubMed]
  • DAVIS BD, MINGIOLI ES. Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950 Jul;60(1):17–28. [PMC free article] [PubMed]
  • Defais M, Fauquet P, Radman M, Errera M. Ultraviolet reactivation and ultraviolet mutagenesis of lambda in different genetic systems. Virology. 1971 Feb;43(2):495–503. [PubMed]
  • Eckardt F, Haynes RH. Kinetics of mutation induction by ultraviolet light in excision-deficient yeast. Genetics. 1977 Feb;85(2):225–247. [PMC free article] [PubMed]
  • Howard-Flanders P, Theriot L. Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics. 1966 Jun;53(6):1137–1150. [PMC free article] [PubMed]
  • Kondo S, Ichikawa H, Iwo K, Kato T. Base-change mutagenesis and prophage induction in strains of Escherichia coli with different DNA repair capacities. Genetics. 1970 Oct;66(2):187–217. [PMC free article] [PubMed]
  • Nishioka H, Doudney CO. Different modes of loss of photoreversibility of mutation and lethal damage in ultraviolet-light resistant and sensitive bacteria. Mutat Res. 1969 Sep-Oct;8(2):215–228. [PubMed]
  • Radman M. SOS repair hypothesis: phenomenology of an inducible DNA repair which is accompanied by mutagenesis. Basic Life Sci. 1975;5A:355–367. [PubMed]
  • Rolfe B, Schell J, Becker A, Heip J, Onodera K, Schell-Frederick E. A colicin-tolerant mutant of Escherichia coli with reduced levels of cyclic AMP and a strong bias towards lambda lysogeny. Mol Gen Genet. 1973 Jan 18;120(1):1–16. [PubMed]
  • Smith KC, Meun DH. Repair of radiation-induced damage in Escherichia coli. I. Effect of rec mutations on post-replication repair of damage due to ultraviolet radiation. J Mol Biol. 1970 Aug;51(3):459–472. [PubMed]

Articles from Genetics are provided here courtesy of Genetics Society of America

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...