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Genetics. Jun 1976; 83(2): 285–301.
PMCID: PMC1213514

Effect of Genes Controlling Radiation Sensitivity on Chemically Induced Mutations in SACCHAROMYCES CEREVISIAE


The effect of 16 different genes (rad) conferring radiation sensitivity on chemically induced reversion in the yeast Saccharomyces cerevisiae was determined. The site of reversion used was a well-defined chain initiation mutant mapping in the structural gene coding for iso-1-cytochrome c. High doses of EMS and HNO2 resulted in decreased reversion of cyc1–131 in rad6, rad9 and rad15 strains compared to the normal RAD+ strains. In addition, rad52 greatly decreased EMS reversion of cyc1–131 but had not effect on HNO 2-induced reversion; rad18, on the other hand, increased HNO 2-induced reversion but did not alter EMS-induced reversion. When NQO was used as the mutagen, every rad gene tested, except for rad14 , had an effect on reversion; rad6, rad9, rad15, rad17, rad18, rad22, rev1, rev2 and rev3 lowered NQO reversion while rad1, rad2, rad3, rad4, rad10, rad12 and rad16 increased it compared to the RAD+ strain. The effect of rad genes on chemical mutagenesis is discussed in terms of their effect on UV mutagenesis. It is concluded that although the nature of the repair pathways may differ for UV- and chemically-induced mutations in yeast, a functional repair system is required for the induction of mutation by the chemical agents NQO, EMS and HNO2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Averbeck D, Laskowski W, Eckardt F, Lehmann-Brauns E. Four radiation sensitive mutants of Saccharomyces. Survival after UV- and x-ray-irradiation as well as UV-induced reversion rates from isoleucine-valine dependence to independence. Mol Gen Genet. 1970;107(2):117–127. [PubMed]
  • Bridges BA, Law J, Munson RJ. Mutagenesis in Escherichia coli. II. Evidence for a common pathway for mutagenesis by ultraviolet light, ionizing radiation and thymine deprivation. Mol Gen Genet. 1968;103(3):266–273. [PubMed]
  • Cox BS, Parry JM. The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutat Res. 1968 Jul-Aug;6(1):37–55. [PubMed]
  • Ishii Y, Kondo S. Comparative analysis of deletion and base-change mutabilities of Escherichia coli B strains differing in DNA repair capacity (wild-type, uvrA-, polA-, recA-) by various mutagens. Mutat Res. 1975 Jan;27(1):27–44. [PubMed]
  • Lawrence CW, Stewart JW, Sherman F, Christensen R. Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeast. J Mol Biol. 1974 May 5;85(1):137–162. [PubMed]
  • Parry EM, Parry JM, Waters R. Genetic and physiological analysis of UV-sensitive mutants of Saccharomyces cerevisiae. Mutat Res. 1972 Jun;15(2):135–146. [PubMed]
  • Prakash L. Lack of chemically induced mutation in repair-deficient mutants of yeast. Genetics. 1974 Dec;78(4):1101–1118. [PMC free article] [PubMed]
  • Resnick MA, Setlow JK. Repair of pyrimidine dimer damage induced in yeast by ultraviolet light. J Bacteriol. 1972 Mar;109(3):979–986. [PMC free article] [PubMed]
  • Sherman F, Stewart JW, Jackson M, Gilmore RA, Parker JH. Mutants of yeast defective in iso-1-cytochrome c. Genetics. 1974 Jun;77(2):255–284. [PMC free article] [PubMed]
  • Zakharov IA, Kozina TN, Fedorova IV. Effets de mutations vers la sensibilité au rayonnement ultraviolet chez la levure. Mutat Res. 1970 Jan;9(1):31–39. [PubMed]

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